Establishing a new human lung squamous cell carcinoma cell line, OMUL‐1, expressing insulin‐like growth factor 1 receptor and programmed cell death ligand 1
Abstract The Main Problem Squamous cell carcinoma is the second most prevalent type of non–small cell lung cancer. Analyzing the molecular mechanisms underlying lung carcinoma requires useful tools, such as squamous lung cancer cell lines. Methods A novel new lung squamous cell carcinoma cell line,...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Thoracic Cancer |
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| Online Access: | https://doi.org/10.1111/1759-7714.15488 |
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| author | Hiroaki Nagamine Masakazu Yashiro Megumi Mizutani Akira Sugimoto Yoshiya Matsumoto Yoko Tani Hiroyasu Kaneda Kazuhiro Yamada Tetsuya Watanabe Kazuhisa Asai Satoshi Suzuki Tomoya Kawaguchi |
| author_facet | Hiroaki Nagamine Masakazu Yashiro Megumi Mizutani Akira Sugimoto Yoshiya Matsumoto Yoko Tani Hiroyasu Kaneda Kazuhiro Yamada Tetsuya Watanabe Kazuhisa Asai Satoshi Suzuki Tomoya Kawaguchi |
| author_sort | Hiroaki Nagamine |
| collection | DOAJ |
| description | Abstract The Main Problem Squamous cell carcinoma is the second most prevalent type of non–small cell lung cancer. Analyzing the molecular mechanisms underlying lung carcinoma requires useful tools, such as squamous lung cancer cell lines. Methods A novel new lung squamous cell carcinoma cell line, OMUL‐1, was developed from the primary lung cancer of a 74‐year‐old man. We assessed the characteristics and behavior of OMUL‐1 cells were examined, including their growth kinetics, tumorigenicity in mice, histological properties, gene expression profiles using reverse transcription polymerase chain reaction (RT‐PCR), and RNA sequencing and invasion assays. Results OMUL‐1‐an adherent cell line‐resulted in 100% tumor formation when subcutaneously injected into mice. Histological analysis of the subcutaneous tumor using hematoxylin and eosin staining revealed squamous cell carcinoma with characteristics similar to those of the primary tumor (p40 and p63 were positive, and TTF‐1 was negative). An invasion assay demonstrated that OMUL‐1 had a lower invasion ability compared to that of other developed cell lines. RT‐PCR analysis and RNA sequencing indicated that OMUL‐1 cells expressed FGFR1, FGFR2, FGFR3, FGFR4, EGFR, HER2, ErbB3, ErbB4, VEGFR3, IGF1R, c‐MET, PDGFRa, and PDGFRb. Additionally, picropodophyllin (an IGF1R inhibitor) significantly inhibited the growth of OMUL‐1 cells. Immunohistochemistry revealed that IGF1R and PD‐L1 were expressed in both the primary and subcutaneous tumors. Conclusions We developed a novel new squamous cell lung carcinoma cell line, OMUL‐1, that expresses IGF1R and PD‐L1. |
| format | Article |
| id | doaj-art-5cdf0614865742409c71621fc7bc147d |
| institution | DOAJ |
| issn | 1759-7706 1759-7714 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Thoracic Cancer |
| spelling | doaj-art-5cdf0614865742409c71621fc7bc147d2025-08-20T02:59:14ZengWileyThoracic Cancer1759-77061759-77142025-01-01161n/an/a10.1111/1759-7714.15488Establishing a new human lung squamous cell carcinoma cell line, OMUL‐1, expressing insulin‐like growth factor 1 receptor and programmed cell death ligand 1Hiroaki Nagamine0Masakazu Yashiro1Megumi Mizutani2Akira Sugimoto3Yoshiya Matsumoto4Yoko Tani5Hiroyasu Kaneda6Kazuhiro Yamada7Tetsuya Watanabe8Kazuhisa Asai9Satoshi Suzuki10Tomoya Kawaguchi11Department of Respiratory Medicine, Graduate School of Medicine Osaka Metropolitan University Osaka JapanMolecular Oncology and Therapeutics, Graduate School of Medicine Osaka Metropolitan University Osaka JapanDepartment of Respiratory Medicine, Graduate School of Medicine Osaka Metropolitan University Osaka JapanDepartment of Respiratory Medicine, Graduate School of Medicine Osaka Metropolitan University Osaka JapanDepartment of Respiratory Medicine, Graduate School of Medicine Osaka Metropolitan University Osaka JapanDepartment of Clinical Oncology, Graduate School of Medicine Osaka Metropolitan University Osaka JapanDepartment of Clinical Oncology, Graduate School of Medicine Osaka Metropolitan University Osaka JapanDepartment of Respiratory Medicine, Graduate School of Medicine Osaka Metropolitan University Osaka JapanDepartment of Respiratory Medicine, Graduate School of Medicine Osaka Metropolitan University Osaka JapanDepartment of Respiratory Medicine, Graduate School of Medicine Osaka Metropolitan University Osaka JapanDepartment of Thoracic Surgery, Graduate School of Medicine Osaka Metropolitan University Osaka JapanDepartment of Respiratory Medicine, Graduate School of Medicine Osaka Metropolitan University Osaka JapanAbstract The Main Problem Squamous cell carcinoma is the second most prevalent type of non–small cell lung cancer. Analyzing the molecular mechanisms underlying lung carcinoma requires useful tools, such as squamous lung cancer cell lines. Methods A novel new lung squamous cell carcinoma cell line, OMUL‐1, was developed from the primary lung cancer of a 74‐year‐old man. We assessed the characteristics and behavior of OMUL‐1 cells were examined, including their growth kinetics, tumorigenicity in mice, histological properties, gene expression profiles using reverse transcription polymerase chain reaction (RT‐PCR), and RNA sequencing and invasion assays. Results OMUL‐1‐an adherent cell line‐resulted in 100% tumor formation when subcutaneously injected into mice. Histological analysis of the subcutaneous tumor using hematoxylin and eosin staining revealed squamous cell carcinoma with characteristics similar to those of the primary tumor (p40 and p63 were positive, and TTF‐1 was negative). An invasion assay demonstrated that OMUL‐1 had a lower invasion ability compared to that of other developed cell lines. RT‐PCR analysis and RNA sequencing indicated that OMUL‐1 cells expressed FGFR1, FGFR2, FGFR3, FGFR4, EGFR, HER2, ErbB3, ErbB4, VEGFR3, IGF1R, c‐MET, PDGFRa, and PDGFRb. Additionally, picropodophyllin (an IGF1R inhibitor) significantly inhibited the growth of OMUL‐1 cells. Immunohistochemistry revealed that IGF1R and PD‐L1 were expressed in both the primary and subcutaneous tumors. Conclusions We developed a novel new squamous cell lung carcinoma cell line, OMUL‐1, that expresses IGF1R and PD‐L1.https://doi.org/10.1111/1759-7714.15488cell lineIGF1Rlung cancerPD‐L1squamous cell carcinoma |
| spellingShingle | Hiroaki Nagamine Masakazu Yashiro Megumi Mizutani Akira Sugimoto Yoshiya Matsumoto Yoko Tani Hiroyasu Kaneda Kazuhiro Yamada Tetsuya Watanabe Kazuhisa Asai Satoshi Suzuki Tomoya Kawaguchi Establishing a new human lung squamous cell carcinoma cell line, OMUL‐1, expressing insulin‐like growth factor 1 receptor and programmed cell death ligand 1 Thoracic Cancer cell line IGF1R lung cancer PD‐L1 squamous cell carcinoma |
| title | Establishing a new human lung squamous cell carcinoma cell line, OMUL‐1, expressing insulin‐like growth factor 1 receptor and programmed cell death ligand 1 |
| title_full | Establishing a new human lung squamous cell carcinoma cell line, OMUL‐1, expressing insulin‐like growth factor 1 receptor and programmed cell death ligand 1 |
| title_fullStr | Establishing a new human lung squamous cell carcinoma cell line, OMUL‐1, expressing insulin‐like growth factor 1 receptor and programmed cell death ligand 1 |
| title_full_unstemmed | Establishing a new human lung squamous cell carcinoma cell line, OMUL‐1, expressing insulin‐like growth factor 1 receptor and programmed cell death ligand 1 |
| title_short | Establishing a new human lung squamous cell carcinoma cell line, OMUL‐1, expressing insulin‐like growth factor 1 receptor and programmed cell death ligand 1 |
| title_sort | establishing a new human lung squamous cell carcinoma cell line omul 1 expressing insulin like growth factor 1 receptor and programmed cell death ligand 1 |
| topic | cell line IGF1R lung cancer PD‐L1 squamous cell carcinoma |
| url | https://doi.org/10.1111/1759-7714.15488 |
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