In vivo polarisation sensitive optical coherence tomography for fibrosis assessment in interstitial lung disease: a prospective, exploratory, observational study
Introduction Endobronchial polarisation sensitive optical coherence tomography (EB-PS-OCT) is a bronchoscopic imaging technique exceeding resolution of high-resolution CT (HRCT) by 50-fold. It detects collagen birefringence, enabling identification and quantification of fibrosis.Study aim To assess...
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| Language: | English |
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BMJ Publishing Group
2023-07-01
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| Series: | BMJ Open Respiratory Research |
| Online Access: | https://bmjopenrespres.bmj.com/content/10/1/e001628.full |
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| author | Michael W T Tanck Onno M Mets Erik Thunnissen Jouke T Annema Peter I Bonta Inge A H van den Berk Margherita Vaselli Kirsten Kalverda-Mooij Johannes F de Boer |
| author_facet | Michael W T Tanck Onno M Mets Erik Thunnissen Jouke T Annema Peter I Bonta Inge A H van den Berk Margherita Vaselli Kirsten Kalverda-Mooij Johannes F de Boer |
| author_sort | Michael W T Tanck |
| collection | DOAJ |
| description | Introduction Endobronchial polarisation sensitive optical coherence tomography (EB-PS-OCT) is a bronchoscopic imaging technique exceeding resolution of high-resolution CT (HRCT) by 50-fold. It detects collagen birefringence, enabling identification and quantification of fibrosis.Study aim To assess pulmonary fibrosis in interstitial lung diseases (ILD) patients with in vivo EB-PS-OCT using histology as reference standard.Primary objective Visualisation and quantification of pulmonary fibrosis by EB-PS-OCT.Secondary objectives Comparison of EB-PS-OCT and HRCT detected fibrosis with histology, identification of ILD histological features in EB-PS-OCT images and comparison of ex vivo PS-OCT results with histology.Methods Observational prospective exploratory study. Patients with ILD scheduled for transbronchial cryobiopsy or surgical lung biopsy underwent in vivo EB-PS-OCT imaging prior to tissue acquisition. Asthma patients were included as non-fibrotic controls. Per imaged lung segment, fibrosis was automatically quantified assessing the birefringent area in EB-PS-OCT images. Fibrotic extent in corresponding HRCT areas and biopsies were compared with EB-PS-OCT detected fibrosis. Microscopic ILD features were identified on EB-PS-OCT images and matched with biopsies from the same segment.Results 19 patients were included (16 ILD; 3 asthma). In 49 in vivo imaged airway segments the parenchymal birefringent area was successfully quantified and ranged from 2.54% (no to minimal fibrosis) to 21.01% (extensive fibrosis). Increased EB-PS-OCT detected birefringent area corresponded to increased histologically confirmed fibrosis, with better predictive value than HRCT. Microscopic ILD features were identified on both in vivo and ex vivo PS-OCT images.Conclusions EB-PS-OCT enables pulmonary fibrosis quantification, thereby has potential to serve as an add-on bronchoscopic imaging technique to diagnose and detect (early) fibrosis in ILD. |
| format | Article |
| id | doaj-art-5cd12ee71d6c4ff7923b1b0b0b041585 |
| institution | DOAJ |
| issn | 2052-4439 |
| language | English |
| publishDate | 2023-07-01 |
| publisher | BMJ Publishing Group |
| record_format | Article |
| series | BMJ Open Respiratory Research |
| spelling | doaj-art-5cd12ee71d6c4ff7923b1b0b0b0415852025-08-20T02:57:59ZengBMJ Publishing GroupBMJ Open Respiratory Research2052-44392023-07-0110110.1136/bmjresp-2023-001628In vivo polarisation sensitive optical coherence tomography for fibrosis assessment in interstitial lung disease: a prospective, exploratory, observational studyMichael W T Tanck0Onno M Mets1Erik Thunnissen2Jouke T Annema3Peter I Bonta4Inge A H van den Berk5Margherita Vaselli6Kirsten Kalverda-Mooij7Johannes F de Boer8Department of Clinical Epidemiology, Biostatistics and Bioinformatics, Amsterdam UMC, University of Amsterdam, Amsterdam, NetherlandsDepartment of Radiology and Nuclear Medicine, University of Amsterdam, Amsterdam, The NetherlandsDepartment of Pathology, Amsterdam University Medical Centra, Amsterdam, The NetherlandsRespiratory Medicine, Amsterdam UMC - Locatie AMC, Amsterdam, The NetherlandsDepartment of Pulmonary Medicine, Amsterdam UMC, University of Amsterdam, Amsterdam, The NetherlandsDepartment of Radiology and Nuclear Medicine, Amsterdam UMC Location AMC, Amsterdam, The NetherlandsDepartment of Physics and Astronomy, Vrije Universiteit Amsterdam, Amsterdam, The NetherlandsRespiratory Medicine, Amsterdam University Medical Centres, Amsterdam, The NetherlandsDepartment of Physics and Astronomy, Vrije Universiteit Amsterdam, Amsterdam, The NetherlandsIntroduction Endobronchial polarisation sensitive optical coherence tomography (EB-PS-OCT) is a bronchoscopic imaging technique exceeding resolution of high-resolution CT (HRCT) by 50-fold. It detects collagen birefringence, enabling identification and quantification of fibrosis.Study aim To assess pulmonary fibrosis in interstitial lung diseases (ILD) patients with in vivo EB-PS-OCT using histology as reference standard.Primary objective Visualisation and quantification of pulmonary fibrosis by EB-PS-OCT.Secondary objectives Comparison of EB-PS-OCT and HRCT detected fibrosis with histology, identification of ILD histological features in EB-PS-OCT images and comparison of ex vivo PS-OCT results with histology.Methods Observational prospective exploratory study. Patients with ILD scheduled for transbronchial cryobiopsy or surgical lung biopsy underwent in vivo EB-PS-OCT imaging prior to tissue acquisition. Asthma patients were included as non-fibrotic controls. Per imaged lung segment, fibrosis was automatically quantified assessing the birefringent area in EB-PS-OCT images. Fibrotic extent in corresponding HRCT areas and biopsies were compared with EB-PS-OCT detected fibrosis. Microscopic ILD features were identified on EB-PS-OCT images and matched with biopsies from the same segment.Results 19 patients were included (16 ILD; 3 asthma). In 49 in vivo imaged airway segments the parenchymal birefringent area was successfully quantified and ranged from 2.54% (no to minimal fibrosis) to 21.01% (extensive fibrosis). Increased EB-PS-OCT detected birefringent area corresponded to increased histologically confirmed fibrosis, with better predictive value than HRCT. Microscopic ILD features were identified on both in vivo and ex vivo PS-OCT images.Conclusions EB-PS-OCT enables pulmonary fibrosis quantification, thereby has potential to serve as an add-on bronchoscopic imaging technique to diagnose and detect (early) fibrosis in ILD.https://bmjopenrespres.bmj.com/content/10/1/e001628.full |
| spellingShingle | Michael W T Tanck Onno M Mets Erik Thunnissen Jouke T Annema Peter I Bonta Inge A H van den Berk Margherita Vaselli Kirsten Kalverda-Mooij Johannes F de Boer In vivo polarisation sensitive optical coherence tomography for fibrosis assessment in interstitial lung disease: a prospective, exploratory, observational study BMJ Open Respiratory Research |
| title | In vivo polarisation sensitive optical coherence tomography for fibrosis assessment in interstitial lung disease: a prospective, exploratory, observational study |
| title_full | In vivo polarisation sensitive optical coherence tomography for fibrosis assessment in interstitial lung disease: a prospective, exploratory, observational study |
| title_fullStr | In vivo polarisation sensitive optical coherence tomography for fibrosis assessment in interstitial lung disease: a prospective, exploratory, observational study |
| title_full_unstemmed | In vivo polarisation sensitive optical coherence tomography for fibrosis assessment in interstitial lung disease: a prospective, exploratory, observational study |
| title_short | In vivo polarisation sensitive optical coherence tomography for fibrosis assessment in interstitial lung disease: a prospective, exploratory, observational study |
| title_sort | in vivo polarisation sensitive optical coherence tomography for fibrosis assessment in interstitial lung disease a prospective exploratory observational study |
| url | https://bmjopenrespres.bmj.com/content/10/1/e001628.full |
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