Hypoglycemic, Antioxidant Activities, and Probiotic Characteristics of <i>Lacticaseibacillus rhamnosus</i> LBUX2302 Isolated from Stool Samples of Neonates
<i>Lacticaseibacillus</i> species have shown potential in managing hyperglycemia, hypercholesterolemia, and oxidative stress, depending on the strain and species. This study aimed to isolate a novel <i>Lacticaseibacillus rhamnosus</i> strain from healthy newborns and assess i...
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2025-05-01
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| author | Pedro A. Reyes-Castillo Ana Laura Esquivel-Campos Edgar Torres-Maravilla Eduardo Zúñiga-León Felipe Mendoza-Pérez Rosa González-Vázquez María Guadalupe Córdova-Espinoza María Angélica Gutiérrez-Nava Raquel González-Vázquez Lino Mayorga-Reyes |
| author_facet | Pedro A. Reyes-Castillo Ana Laura Esquivel-Campos Edgar Torres-Maravilla Eduardo Zúñiga-León Felipe Mendoza-Pérez Rosa González-Vázquez María Guadalupe Córdova-Espinoza María Angélica Gutiérrez-Nava Raquel González-Vázquez Lino Mayorga-Reyes |
| author_sort | Pedro A. Reyes-Castillo |
| collection | DOAJ |
| description | <i>Lacticaseibacillus</i> species have shown potential in managing hyperglycemia, hypercholesterolemia, and oxidative stress, depending on the strain and species. This study aimed to isolate a novel <i>Lacticaseibacillus rhamnosus</i> strain from healthy newborns and assess its hypoglycemic and antioxidative activity, along with other probiotic properties. A non-hemolytic <i>L. rhamnosus</i> LBUX2302 was isolated, and it exhibited survival rates of 2.7%, 22%, and 27.5% at pH 2, 3, and 5 for 120 min. It metabolized various carbon sources and showed resistance to gentamicin, dicloxacillin, and penicillin; coaggregated with <i>Salmonella typhi</i> ATCC14028, <i>Staphylococcus aureus</i> STCC6538, and <i>Escherichia coli</i> O157:H7. <i>L. rhamnosus</i> LBUX2302 showed hydrophobicity, autoaggregation, and adhesion to HaCat, HeLa, MCF-7, SK-LU-1, and SW620 cell lines. It also exhibited extracellular activity of bile salt hydrolase. Enzymatic inhibition assays revealed 66% and 24% inhibitions of α-amylase and α-glucosidase, respectively. Its cell-free supernatant inhibited DPPH (89%), hydroxyl (81%), and superoxide anion radicals (61%). Also, antioxidant activity was observed in whole cells and cell fragments. Finally, the presence of ferulic acid activity was detected. The results highlight <i>L. rhamnosus</i> LBUX2302 as a promising probiotic with hypoglycemic and antioxidant effects, warranting further in vivo evaluation for its possible inclusion in functional food and health formulations. |
| format | Article |
| id | doaj-art-5cce24f4389741cc82a992b0794a3035 |
| institution | DOAJ |
| issn | 2075-1729 |
| language | English |
| publishDate | 2025-05-01 |
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| spelling | doaj-art-5cce24f4389741cc82a992b0794a30352025-08-20T03:14:39ZengMDPI AGLife2075-17292025-05-0115580410.3390/life15050804Hypoglycemic, Antioxidant Activities, and Probiotic Characteristics of <i>Lacticaseibacillus rhamnosus</i> LBUX2302 Isolated from Stool Samples of NeonatesPedro A. Reyes-Castillo0Ana Laura Esquivel-Campos1Edgar Torres-Maravilla2Eduardo Zúñiga-León3Felipe Mendoza-Pérez4Rosa González-Vázquez5María Guadalupe Córdova-Espinoza6María Angélica Gutiérrez-Nava7Raquel González-Vázquez8Lino Mayorga-Reyes9Doctorado en Ciencias Biologicas y de la Salud, Universidad Autonoma Metropolitana, Mexico City 04960, MexicoLaboratorio de Biotecnologia, Departamento de Sistemas Biologicos, Universidad Autonoma Metropolitana Unidad Xochimilco, Mexico City 04960, MexicoFacultad de Medicina Mexicali, Universidad Autonoma de Baja California, Mexicali 21000, MexicoLaboratorio de Biotecnologia, Departamento de Sistemas Biologicos, Universidad Autonoma Metropolitana Unidad Xochimilco, Mexico City 04960, MexicoLaboratorio de Biotecnologia, Departamento de Sistemas Biologicos, Universidad Autonoma Metropolitana Unidad Xochimilco, Mexico City 04960, MexicoLaboratorio de Bacteriologia Medica, Escuela Nacional de Ciencias Biologicas, Instituto Politecnico Nacional (IPN), Mexico City 11350, MexicoLaboratorio de Bacteriologia Medica, Escuela Nacional de Ciencias Biologicas, Instituto Politecnico Nacional (IPN), Mexico City 11350, MexicoLaboratorio de Ecologia Microbiana, Departamento de Sistemas Biologicos, Universidad Autonoma Metropolitana Unidad Xochimilco, Ciudad de Mexico 04960, MexicoLaboratorio de Biotecnologia, Departamento de Sistemas Biologicos, Secihti-Universidad Autonoma Metropolitana Unidad Xochimilco, Mexico City 04960, MexicoLaboratorio de Biotecnologia, Departamento de Sistemas Biologicos, Universidad Autonoma Metropolitana Unidad Xochimilco, Mexico City 04960, Mexico<i>Lacticaseibacillus</i> species have shown potential in managing hyperglycemia, hypercholesterolemia, and oxidative stress, depending on the strain and species. This study aimed to isolate a novel <i>Lacticaseibacillus rhamnosus</i> strain from healthy newborns and assess its hypoglycemic and antioxidative activity, along with other probiotic properties. A non-hemolytic <i>L. rhamnosus</i> LBUX2302 was isolated, and it exhibited survival rates of 2.7%, 22%, and 27.5% at pH 2, 3, and 5 for 120 min. It metabolized various carbon sources and showed resistance to gentamicin, dicloxacillin, and penicillin; coaggregated with <i>Salmonella typhi</i> ATCC14028, <i>Staphylococcus aureus</i> STCC6538, and <i>Escherichia coli</i> O157:H7. <i>L. rhamnosus</i> LBUX2302 showed hydrophobicity, autoaggregation, and adhesion to HaCat, HeLa, MCF-7, SK-LU-1, and SW620 cell lines. It also exhibited extracellular activity of bile salt hydrolase. Enzymatic inhibition assays revealed 66% and 24% inhibitions of α-amylase and α-glucosidase, respectively. Its cell-free supernatant inhibited DPPH (89%), hydroxyl (81%), and superoxide anion radicals (61%). Also, antioxidant activity was observed in whole cells and cell fragments. Finally, the presence of ferulic acid activity was detected. The results highlight <i>L. rhamnosus</i> LBUX2302 as a promising probiotic with hypoglycemic and antioxidant effects, warranting further in vivo evaluation for its possible inclusion in functional food and health formulations.https://www.mdpi.com/2075-1729/15/5/804<i>Lacticaseibacillus rhamnosus</i>probioticsbile salt hydrolase activityantioxidant effectadhesion |
| spellingShingle | Pedro A. Reyes-Castillo Ana Laura Esquivel-Campos Edgar Torres-Maravilla Eduardo Zúñiga-León Felipe Mendoza-Pérez Rosa González-Vázquez María Guadalupe Córdova-Espinoza María Angélica Gutiérrez-Nava Raquel González-Vázquez Lino Mayorga-Reyes Hypoglycemic, Antioxidant Activities, and Probiotic Characteristics of <i>Lacticaseibacillus rhamnosus</i> LBUX2302 Isolated from Stool Samples of Neonates Life <i>Lacticaseibacillus rhamnosus</i> probiotics bile salt hydrolase activity antioxidant effect adhesion |
| title | Hypoglycemic, Antioxidant Activities, and Probiotic Characteristics of <i>Lacticaseibacillus rhamnosus</i> LBUX2302 Isolated from Stool Samples of Neonates |
| title_full | Hypoglycemic, Antioxidant Activities, and Probiotic Characteristics of <i>Lacticaseibacillus rhamnosus</i> LBUX2302 Isolated from Stool Samples of Neonates |
| title_fullStr | Hypoglycemic, Antioxidant Activities, and Probiotic Characteristics of <i>Lacticaseibacillus rhamnosus</i> LBUX2302 Isolated from Stool Samples of Neonates |
| title_full_unstemmed | Hypoglycemic, Antioxidant Activities, and Probiotic Characteristics of <i>Lacticaseibacillus rhamnosus</i> LBUX2302 Isolated from Stool Samples of Neonates |
| title_short | Hypoglycemic, Antioxidant Activities, and Probiotic Characteristics of <i>Lacticaseibacillus rhamnosus</i> LBUX2302 Isolated from Stool Samples of Neonates |
| title_sort | hypoglycemic antioxidant activities and probiotic characteristics of i lacticaseibacillus rhamnosus i lbux2302 isolated from stool samples of neonates |
| topic | <i>Lacticaseibacillus rhamnosus</i> probiotics bile salt hydrolase activity antioxidant effect adhesion |
| url | https://www.mdpi.com/2075-1729/15/5/804 |
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