Effects of hyperbaric oxygen and N-acetylcysteine in survival of random pattern skin flaps in rats
Objective: Our aim is to investigate the role of HBO (hyperbaric oxygen), NAC (N-acetylcysteine), and HBO plus NAC on the necrosis area of random rat′s skin flaps of a modified McFarlane flap design. Materials and Methods: Thirty-two male Wistar rats were randomly divided into four groups: G-S (sham...
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Thieme Medical and Scientific Publishers Pvt. Ltd.
2012-09-01
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| Series: | Indian Journal of Plastic Surgery |
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| Online Access: | http://www.thieme-connect.de/DOI/DOI?10.4103/0970-0358.105941 |
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| author | Fernando da Rocha Djalma Fagundes Jefferson Pires Fernanda Salim da Rocha |
| author_facet | Fernando da Rocha Djalma Fagundes Jefferson Pires Fernanda Salim da Rocha |
| author_sort | Fernando da Rocha |
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| description | Objective: Our aim is to investigate the role of HBO (hyperbaric oxygen), NAC (N-acetylcysteine), and HBO plus NAC on the necrosis area of random rat′s skin flaps of a modified McFarlane flap design. Materials and Methods: Thirty-two male Wistar rats were randomly divided into four groups: G-S (sham: n = 8), G-NAC (NAC: n = 8), G-HBO (HBO: n = 8), and G-HN (HBO plus NAC: n = 8). A rectangular skin flap (2 × 8 cm 2 ) was dissected from the muscular dorsal layer, preserving the cranial pedicle. Polyethylene film was placed over the muscular layer and an interrupted 3.0 nylon suture was employed to fix the flap into the original place. On the eighth day, full-thickness biopsy samples (2 × 1 cm 2 ) were collected from the proximal, middle, and cranial areas of the skin flap, and in a site away from the flap labelled as the control area. Results: The measurements of necrotic areas in the groups were 18.3% in G-S, 24.3% in G-NAC, 12.6% in G-HBO, and 14.9% in G-HN. Significant difference was observed between the groups G-HBO and G-HN as well as G-NAC. Conclusion: HBO is associated with reduced area of necrosis of skin flap. The G-NAC group was associated with poor results when examined in isolation. The association between HBO and NAC did not produce favourable results with respect to the use of HBO alone. These findings suggest that the diffusion of oxygen through the interstitial space was the determining factor of more favourable results of HBO. |
| format | Article |
| id | doaj-art-5cc96130422347c19239cea24386fa3e |
| institution | OA Journals |
| issn | 0970-0358 1998-376X |
| language | English |
| publishDate | 2012-09-01 |
| publisher | Thieme Medical and Scientific Publishers Pvt. Ltd. |
| record_format | Article |
| series | Indian Journal of Plastic Surgery |
| spelling | doaj-art-5cc96130422347c19239cea24386fa3e2025-08-20T02:04:59ZengThieme Medical and Scientific Publishers Pvt. Ltd.Indian Journal of Plastic Surgery0970-03581998-376X2012-09-01450345345810.4103/0970-0358.105941Effects of hyperbaric oxygen and N-acetylcysteine in survival of random pattern skin flaps in ratsFernando da Rocha0Djalma Fagundes1Jefferson Pires2Fernanda Salim da Rocha3Department of Surgery, Catholic University of Pelotas, Pelotas - RSDivision of Operative Technique and Experimental Surgery, Department of Surgery, UNIFESPPresident of Academy League of Plastic Surgery, Federal University of Pelotas, Pelotas - RSPresident of Academy League of Plastic Surgery, Federal University of Pelotas, Pelotas - RS, BrazilObjective: Our aim is to investigate the role of HBO (hyperbaric oxygen), NAC (N-acetylcysteine), and HBO plus NAC on the necrosis area of random rat′s skin flaps of a modified McFarlane flap design. Materials and Methods: Thirty-two male Wistar rats were randomly divided into four groups: G-S (sham: n = 8), G-NAC (NAC: n = 8), G-HBO (HBO: n = 8), and G-HN (HBO plus NAC: n = 8). A rectangular skin flap (2 × 8 cm 2 ) was dissected from the muscular dorsal layer, preserving the cranial pedicle. Polyethylene film was placed over the muscular layer and an interrupted 3.0 nylon suture was employed to fix the flap into the original place. On the eighth day, full-thickness biopsy samples (2 × 1 cm 2 ) were collected from the proximal, middle, and cranial areas of the skin flap, and in a site away from the flap labelled as the control area. Results: The measurements of necrotic areas in the groups were 18.3% in G-S, 24.3% in G-NAC, 12.6% in G-HBO, and 14.9% in G-HN. Significant difference was observed between the groups G-HBO and G-HN as well as G-NAC. Conclusion: HBO is associated with reduced area of necrosis of skin flap. The G-NAC group was associated with poor results when examined in isolation. The association between HBO and NAC did not produce favourable results with respect to the use of HBO alone. These findings suggest that the diffusion of oxygen through the interstitial space was the determining factor of more favourable results of HBO.http://www.thieme-connect.de/DOI/DOI?10.4103/0970-0358.105941acetylcysteinehyperbaric oxygenationnecrosissurgical flaps |
| spellingShingle | Fernando da Rocha Djalma Fagundes Jefferson Pires Fernanda Salim da Rocha Effects of hyperbaric oxygen and N-acetylcysteine in survival of random pattern skin flaps in rats Indian Journal of Plastic Surgery acetylcysteine hyperbaric oxygenation necrosis surgical flaps |
| title | Effects of hyperbaric oxygen and N-acetylcysteine in survival of random pattern skin flaps in rats |
| title_full | Effects of hyperbaric oxygen and N-acetylcysteine in survival of random pattern skin flaps in rats |
| title_fullStr | Effects of hyperbaric oxygen and N-acetylcysteine in survival of random pattern skin flaps in rats |
| title_full_unstemmed | Effects of hyperbaric oxygen and N-acetylcysteine in survival of random pattern skin flaps in rats |
| title_short | Effects of hyperbaric oxygen and N-acetylcysteine in survival of random pattern skin flaps in rats |
| title_sort | effects of hyperbaric oxygen and n acetylcysteine in survival of random pattern skin flaps in rats |
| topic | acetylcysteine hyperbaric oxygenation necrosis surgical flaps |
| url | http://www.thieme-connect.de/DOI/DOI?10.4103/0970-0358.105941 |
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