Active Forms of Chemerin Are Elevated in Human and Mouse Ovarian Carcinoma

<b>Background:</b> Chemerin is a small adipokine that is activated and inactivated by proteolysis of its C-terminus with a role in regulating metabolism, immunity, and inflammation. Significant levels of chemerin are found in circulation and ascitic fluid of ovarian carcinoma patients. &...

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Main Authors: Lei Zhao, Qin Zhou, Venkatesh Krishnan, Justine Chan, Simone Sasse, Supreeti Tallapragada, Dan Eisenberg, Lawrence Leung, Oliver Dorigo, John Morser
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Biomedicines
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Online Access:https://www.mdpi.com/2227-9059/13/4/991
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author Lei Zhao
Qin Zhou
Venkatesh Krishnan
Justine Chan
Simone Sasse
Supreeti Tallapragada
Dan Eisenberg
Lawrence Leung
Oliver Dorigo
John Morser
author_facet Lei Zhao
Qin Zhou
Venkatesh Krishnan
Justine Chan
Simone Sasse
Supreeti Tallapragada
Dan Eisenberg
Lawrence Leung
Oliver Dorigo
John Morser
author_sort Lei Zhao
collection DOAJ
description <b>Background:</b> Chemerin is a small adipokine that is activated and inactivated by proteolysis of its C-terminus with a role in regulating metabolism, immunity, and inflammation. Significant levels of chemerin are found in circulation and ascitic fluid of ovarian carcinoma patients. <b>Methods:</b> We investigated the levels of different chemerin forms in three cohorts: people with BMI < 25, with BMI > 40, and ovarian carcinoma ascites with ELISAs specific for different chemerin forms. Ascites from a mouse model of ovarian carcinoma were also analyzed, and the model was compared between wild-type and chemerin-deficient mice. <b>Results:</b> Conversion of plasma to serum increased the levels of processed chemerin with lower increases in samples from people with BMI < 25 than in people with BMI > 40. High levels of total chemerin and processed forms of chemerin were found in ascitic fluid from both patients who had a mean BMI of 29 and the mouse model. In chemerin-deficient mice the tumors grew slower than in wild-type mice. <b>Conclusions:</b> Serum has more processed and active chemerin than plasma irrespective of source. Ascites of ovarian carcinoma patients contained high levels of active chemerin, which, based on the mouse data, enhance tumor growth.
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spelling doaj-art-5cc45b55a3f84bb29011bdd2097da7122025-08-20T02:17:19ZengMDPI AGBiomedicines2227-90592025-04-0113499110.3390/biomedicines13040991Active Forms of Chemerin Are Elevated in Human and Mouse Ovarian CarcinomaLei Zhao0Qin Zhou1Venkatesh Krishnan2Justine Chan3Simone Sasse4Supreeti Tallapragada5Dan Eisenberg6Lawrence Leung7Oliver Dorigo8John Morser9Division of Hematology, Stanford University School of Medicine, Stanford, CA 94305, USADivision of Hematology, Stanford University School of Medicine, Stanford, CA 94305, USADivision of Gynecologic Oncology, Department of Obstetrics and Gynecology, Stanford Women’s Cancer Center, Stanford Cancer Institute, Stanford, CA 94305, USADivision of Gynecologic Oncology, Department of Obstetrics and Gynecology, Stanford Women’s Cancer Center, Stanford Cancer Institute, Stanford, CA 94305, USADivision of Gynecologic Oncology, Department of Obstetrics and Gynecology, Stanford Women’s Cancer Center, Stanford Cancer Institute, Stanford, CA 94305, USADivision of Gynecologic Oncology, Department of Obstetrics and Gynecology, Stanford Women’s Cancer Center, Stanford Cancer Institute, Stanford, CA 94305, USAVeterans Affairs Palo Alto Health Care System, Palo Alto, CA 94304, USADivision of Hematology, Stanford University School of Medicine, Stanford, CA 94305, USADivision of Gynecologic Oncology, Department of Obstetrics and Gynecology, Stanford Women’s Cancer Center, Stanford Cancer Institute, Stanford, CA 94305, USAVeterans Affairs Palo Alto Health Care System, Palo Alto, CA 94304, USA<b>Background:</b> Chemerin is a small adipokine that is activated and inactivated by proteolysis of its C-terminus with a role in regulating metabolism, immunity, and inflammation. Significant levels of chemerin are found in circulation and ascitic fluid of ovarian carcinoma patients. <b>Methods:</b> We investigated the levels of different chemerin forms in three cohorts: people with BMI < 25, with BMI > 40, and ovarian carcinoma ascites with ELISAs specific for different chemerin forms. Ascites from a mouse model of ovarian carcinoma were also analyzed, and the model was compared between wild-type and chemerin-deficient mice. <b>Results:</b> Conversion of plasma to serum increased the levels of processed chemerin with lower increases in samples from people with BMI < 25 than in people with BMI > 40. High levels of total chemerin and processed forms of chemerin were found in ascitic fluid from both patients who had a mean BMI of 29 and the mouse model. In chemerin-deficient mice the tumors grew slower than in wild-type mice. <b>Conclusions:</b> Serum has more processed and active chemerin than plasma irrespective of source. Ascites of ovarian carcinoma patients contained high levels of active chemerin, which, based on the mouse data, enhance tumor growth.https://www.mdpi.com/2227-9059/13/4/991chemerinovarian carcinomaprotease cleavage of chemerin
spellingShingle Lei Zhao
Qin Zhou
Venkatesh Krishnan
Justine Chan
Simone Sasse
Supreeti Tallapragada
Dan Eisenberg
Lawrence Leung
Oliver Dorigo
John Morser
Active Forms of Chemerin Are Elevated in Human and Mouse Ovarian Carcinoma
Biomedicines
chemerin
ovarian carcinoma
protease cleavage of chemerin
title Active Forms of Chemerin Are Elevated in Human and Mouse Ovarian Carcinoma
title_full Active Forms of Chemerin Are Elevated in Human and Mouse Ovarian Carcinoma
title_fullStr Active Forms of Chemerin Are Elevated in Human and Mouse Ovarian Carcinoma
title_full_unstemmed Active Forms of Chemerin Are Elevated in Human and Mouse Ovarian Carcinoma
title_short Active Forms of Chemerin Are Elevated in Human and Mouse Ovarian Carcinoma
title_sort active forms of chemerin are elevated in human and mouse ovarian carcinoma
topic chemerin
ovarian carcinoma
protease cleavage of chemerin
url https://www.mdpi.com/2227-9059/13/4/991
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