Effect of <i>Ns</i> Gene Dosage and Temperature on the Level of Potato Resistance to PVS

The aim of this study was to verify the influence of the dosage of the gene <i>Ns (simplex</i> or <i>duplex</i>), derived from <i>S. tuberosum subsp. Andigena</i>, on potato resistance to PVS and to evaluate changes in the response of potatoes depending on the met...

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Main Authors: Beata Tatarowska, Dorota Milczarek, Katarzyna Szajko, Jarosław Plich, Dominika Boguszewska-Mańkowska
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Agriculture
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Online Access:https://www.mdpi.com/2077-0472/15/12/1239
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author Beata Tatarowska
Dorota Milczarek
Katarzyna Szajko
Jarosław Plich
Dominika Boguszewska-Mańkowska
author_facet Beata Tatarowska
Dorota Milczarek
Katarzyna Szajko
Jarosław Plich
Dominika Boguszewska-Mańkowska
author_sort Beata Tatarowska
collection DOAJ
description The aim of this study was to verify the influence of the dosage of the gene <i>Ns (simplex</i> or <i>duplex</i>), derived from <i>S. tuberosum subsp. Andigena</i>, on potato resistance to PVS and to evaluate changes in the response of potatoes depending on the method of inoculation used and different cultivation temperatures. The analyses carried out made it possible to distinguish 42 clones in duplex and 8 in simplex form. The analysis showed that the <i>Ns</i> gene, even in simplex form, confers full resistance to PVS. Our results also suggest that an increase in temperature may weaken the resistance response of host plants carrying the Ns gene. In our research, PVS overcame the resistance conferred by the <i>Ns</i> gene at higher temperatures in three tetraploid clones: <i>Ns</i>-<i>II</i>-<i>3</i>, <i>Ns</i>-<i>II</i>-<i>4</i> and <i>Ns</i>-<i>II</i>-<i>81</i>. These clones were classified as ‘temperature-dependent’ (TD). For clones <i>Ns</i>-<i>II</i>-<i>6</i>, <i>Ns</i>-<i>II</i>-<i>10</i>, <i>Ns</i>-<i>II</i>-<i>40</i> and <i>Ns</i>-<i>II</i>-<i>43</i>, the increase in temperature had no effect on the resistance response of host plants carrying the <i>Ns</i> gene. These clones were classified as ‘resistant’ (R).
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publishDate 2025-06-01
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spelling doaj-art-5cb36295efb24f84a3ba60e9d60f28ce2025-08-20T03:26:22ZengMDPI AGAgriculture2077-04722025-06-011512123910.3390/agriculture15121239Effect of <i>Ns</i> Gene Dosage and Temperature on the Level of Potato Resistance to PVSBeata Tatarowska0Dorota Milczarek1Katarzyna Szajko2Jarosław Plich3Dominika Boguszewska-Mańkowska4Plant Breeding and Acclimatization Institute—National Research Institute in Radzików, Młochów Division, Department of Potato Genetics and Parental Lines, Platanowa Str. 19, 05-831 Młochów, PolandPlant Breeding and Acclimatization Institute—National Research Institute in Radzików, Młochów Division, Department of Potato Genetics and Parental Lines, Platanowa Str. 19, 05-831 Młochów, PolandPlant Breeding and Acclimatization Institute—National Research Institute in Radzików, Młochów Division, Department of Potato Genetics and Parental Lines, Platanowa Str. 19, 05-831 Młochów, PolandPlant Breeding and Acclimatization Institute—National Research Institute in Radzików, Młochów Division, Department of Potato Genetics and Parental Lines, Platanowa Str. 19, 05-831 Młochów, PolandPlant Breeding and Acclimatization Institute—National Research Institute in Radzików, Jadwisin Division, Department of Potato Agronomy, Szaniawskiego Str. 15, 05-140 Serock, PolandThe aim of this study was to verify the influence of the dosage of the gene <i>Ns (simplex</i> or <i>duplex</i>), derived from <i>S. tuberosum subsp. Andigena</i>, on potato resistance to PVS and to evaluate changes in the response of potatoes depending on the method of inoculation used and different cultivation temperatures. The analyses carried out made it possible to distinguish 42 clones in duplex and 8 in simplex form. The analysis showed that the <i>Ns</i> gene, even in simplex form, confers full resistance to PVS. Our results also suggest that an increase in temperature may weaken the resistance response of host plants carrying the Ns gene. In our research, PVS overcame the resistance conferred by the <i>Ns</i> gene at higher temperatures in three tetraploid clones: <i>Ns</i>-<i>II</i>-<i>3</i>, <i>Ns</i>-<i>II</i>-<i>4</i> and <i>Ns</i>-<i>II</i>-<i>81</i>. These clones were classified as ‘temperature-dependent’ (TD). For clones <i>Ns</i>-<i>II</i>-<i>6</i>, <i>Ns</i>-<i>II</i>-<i>10</i>, <i>Ns</i>-<i>II</i>-<i>40</i> and <i>Ns</i>-<i>II</i>-<i>43</i>, the increase in temperature had no effect on the resistance response of host plants carrying the <i>Ns</i> gene. These clones were classified as ‘resistant’ (R).https://www.mdpi.com/2077-0472/15/12/1239dosage genepotato<i>S. tuberosum</i> subsp. <i>andigena</i>gene <i>Ns</i>temperature cultivation
spellingShingle Beata Tatarowska
Dorota Milczarek
Katarzyna Szajko
Jarosław Plich
Dominika Boguszewska-Mańkowska
Effect of <i>Ns</i> Gene Dosage and Temperature on the Level of Potato Resistance to PVS
Agriculture
dosage gene
potato
<i>S. tuberosum</i> subsp. <i>andigena</i>
gene <i>Ns</i>
temperature cultivation
title Effect of <i>Ns</i> Gene Dosage and Temperature on the Level of Potato Resistance to PVS
title_full Effect of <i>Ns</i> Gene Dosage and Temperature on the Level of Potato Resistance to PVS
title_fullStr Effect of <i>Ns</i> Gene Dosage and Temperature on the Level of Potato Resistance to PVS
title_full_unstemmed Effect of <i>Ns</i> Gene Dosage and Temperature on the Level of Potato Resistance to PVS
title_short Effect of <i>Ns</i> Gene Dosage and Temperature on the Level of Potato Resistance to PVS
title_sort effect of i ns i gene dosage and temperature on the level of potato resistance to pvs
topic dosage gene
potato
<i>S. tuberosum</i> subsp. <i>andigena</i>
gene <i>Ns</i>
temperature cultivation
url https://www.mdpi.com/2077-0472/15/12/1239
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AT dorotamilczarek effectofinsigenedosageandtemperatureonthelevelofpotatoresistancetopvs
AT katarzynaszajko effectofinsigenedosageandtemperatureonthelevelofpotatoresistancetopvs
AT jarosławplich effectofinsigenedosageandtemperatureonthelevelofpotatoresistancetopvs
AT dominikaboguszewskamankowska effectofinsigenedosageandtemperatureonthelevelofpotatoresistancetopvs