Human papillomaviruses in cervical specimens of women residing in Riyadh, Saudi Arabia: a hospital-based study
Introduction: Certain genotypes of human papillomavirus (HPV) are linked to cervical abnormalities. HPV DNA and genotype prevalence among women residing in Riyadh, Saudi Arabia is investigated in this hospital-based study. Methodology: Cervical specimens were taken from 519 subjects along with cons...
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| Format: | Article |
| Language: | English |
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The Journal of Infection in Developing Countries
2014-03-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/4220 |
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| author | Mohammed N Al-Ahdal Walaa K Al-Arnous Marie F Bohol Suhair M Abuzaid Mohamed Shoukri Kamal S Elrady Nayyer Firdous Rehab Aliyan Rabia Taseer Amal A Al-Hazzani Ahmed A Al-Qahtani |
| author_facet | Mohammed N Al-Ahdal Walaa K Al-Arnous Marie F Bohol Suhair M Abuzaid Mohamed Shoukri Kamal S Elrady Nayyer Firdous Rehab Aliyan Rabia Taseer Amal A Al-Hazzani Ahmed A Al-Qahtani |
| author_sort | Mohammed N Al-Ahdal |
| collection | DOAJ |
| description | Introduction: Certain genotypes of human papillomavirus (HPV) are linked to cervical abnormalities. HPV DNA and genotype prevalence among women residing in Riyadh, Saudi Arabia is investigated in this hospital-based study.
Methodology: Cervical specimens were taken from 519 subjects along with consent and demographic data. DNA was extracted and PCR was performed on all specimens using general primers. Low- and high-risk HPV genotypes were determined by reverse blot hybridization assay using specific probes. SPSS version 17 was used for the data analysis.
Results: Of 519 cervical specimens, 164 (31.6%) were positive for HPV DNA. There was a significant association between HPV positivity and abnormal cytology (p < 0.00001). Even though the HPV positivity was relatively high, the squamous intraepithelial lesions were minimal, with one low grade and one high grade case among those HPV DNA-positive specimens. Regardless of single or multiple infections per specimen, HPV-16 was found in 87.8%, followed by HPV-18 in 86%, and HPV-11 in 78.3%.
Conclusions: Amplification technology showed that HPV is common among women in Riyadh, Saudi Arabia, with a strong association between HPV infection and cytological changes. HPV-16 was the most frequent genotype but had a low prevalence of cervical cancer.
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| format | Article |
| id | doaj-art-5cafdee6f2f545e88d33e81c7f976c94 |
| institution | DOAJ |
| issn | 1972-2680 |
| language | English |
| publishDate | 2014-03-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-5cafdee6f2f545e88d33e81c7f976c942025-08-20T02:57:53ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802014-03-0180310.3855/jidc.4220Human papillomaviruses in cervical specimens of women residing in Riyadh, Saudi Arabia: a hospital-based studyMohammed N Al-Ahdal0Walaa K Al-Arnous1Marie F Bohol2Suhair M Abuzaid3Mohamed Shoukri4Kamal S Elrady5Nayyer Firdous6Rehab Aliyan7Rabia Taseer8Amal A Al-Hazzani9Ahmed A Al-Qahtani10King Faisal Specialist Hospital and Research Center, Riyadh, Saudi ArabiaKing Faisal Specialist Hospital and Research Center, Riyadh, Saudi ArabiaKing Faisal Specialist Hospital and Research Center, Riyadh, Saudi ArabiaKing Faisal Specialist Hospital and Research Center, Riyadh, Saudi ArabiaKing Faisal Specialist Hospital and Research Center, Riyadh, Saudi ArabiaObeid Specialized Hospital, Riyadh, Saudi ArabiaObeid Specialized Hospital, Riyadh, Saudi ArabiaObeid Specialized Hospital, Riyadh, Saudi ArabiaObeid Specialized Hospital, Riyadh, Saudi ArabiaKing Saud University, Riyadh, Saudi ArabiaKing Faisal Specialist Hospital and Research Center, Riyadh, Saudi ArabiaIntroduction: Certain genotypes of human papillomavirus (HPV) are linked to cervical abnormalities. HPV DNA and genotype prevalence among women residing in Riyadh, Saudi Arabia is investigated in this hospital-based study. Methodology: Cervical specimens were taken from 519 subjects along with consent and demographic data. DNA was extracted and PCR was performed on all specimens using general primers. Low- and high-risk HPV genotypes were determined by reverse blot hybridization assay using specific probes. SPSS version 17 was used for the data analysis. Results: Of 519 cervical specimens, 164 (31.6%) were positive for HPV DNA. There was a significant association between HPV positivity and abnormal cytology (p < 0.00001). Even though the HPV positivity was relatively high, the squamous intraepithelial lesions were minimal, with one low grade and one high grade case among those HPV DNA-positive specimens. Regardless of single or multiple infections per specimen, HPV-16 was found in 87.8%, followed by HPV-18 in 86%, and HPV-11 in 78.3%. Conclusions: Amplification technology showed that HPV is common among women in Riyadh, Saudi Arabia, with a strong association between HPV infection and cytological changes. HPV-16 was the most frequent genotype but had a low prevalence of cervical cancer. https://jidc.org/index.php/journal/article/view/4220HPVinfectiongenotypesRiyadhSaudi Arabia |
| spellingShingle | Mohammed N Al-Ahdal Walaa K Al-Arnous Marie F Bohol Suhair M Abuzaid Mohamed Shoukri Kamal S Elrady Nayyer Firdous Rehab Aliyan Rabia Taseer Amal A Al-Hazzani Ahmed A Al-Qahtani Human papillomaviruses in cervical specimens of women residing in Riyadh, Saudi Arabia: a hospital-based study Journal of Infection in Developing Countries HPV infection genotypes Riyadh Saudi Arabia |
| title | Human papillomaviruses in cervical specimens of women residing in Riyadh, Saudi Arabia: a hospital-based study |
| title_full | Human papillomaviruses in cervical specimens of women residing in Riyadh, Saudi Arabia: a hospital-based study |
| title_fullStr | Human papillomaviruses in cervical specimens of women residing in Riyadh, Saudi Arabia: a hospital-based study |
| title_full_unstemmed | Human papillomaviruses in cervical specimens of women residing in Riyadh, Saudi Arabia: a hospital-based study |
| title_short | Human papillomaviruses in cervical specimens of women residing in Riyadh, Saudi Arabia: a hospital-based study |
| title_sort | human papillomaviruses in cervical specimens of women residing in riyadh saudi arabia a hospital based study |
| topic | HPV infection genotypes Riyadh Saudi Arabia |
| url | https://jidc.org/index.php/journal/article/view/4220 |
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