DETERMINATION OF DIETARY FIBRE – THE INFLUENCE OF MILLING FRACTION AND MIXING PROCESS

Dietary fibre is an important constituent of a healthy diet, composed of non-digestible carbohydrates and lignin. Over the last decades dietary fibre has gained importance for human nutrition, due to its beneficial effects on health. In addition to classical enzyme-gravimetric methods, new methods f...

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Main Authors: Blaž FERJANČIČ, Jasna BERTONCELJ
Format: Article
Language:English
Published: University of Ljubljana Press (Založba Univerze v Ljubljani) 2018-04-01
Series:Acta Agriculturae Slovenica
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Online Access:https://journals.uni-lj.si/aas/article/view/12764
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author Blaž FERJANČIČ
Jasna BERTONCELJ
author_facet Blaž FERJANČIČ
Jasna BERTONCELJ
author_sort Blaž FERJANČIČ
collection DOAJ
description Dietary fibre is an important constituent of a healthy diet, composed of non-digestible carbohydrates and lignin. Over the last decades dietary fibre has gained importance for human nutrition, due to its beneficial effects on health. In addition to classical enzyme-gravimetric methods, new methods for the determination of total, insoluble and soluble dietary fibres in foods have recently been developed, but have not yet been fully implemented for use. For the purpose of creating food composition databases and for food labelling, the classical AOAC 985.29 and 991.43 methods are still widely used. The methods are enzyme-gravimetric and therefore sensitive to enzyme kinetics. The aim of the study was to investigate the effect of milling fraction and mixing of the sample on dietary fibre content determined with the AOAC method 991.43. The results showed that milling fraction significantly influences the content of dietary fibre, especially in unprocessed or slightly processed cereals, the mixing acts synergistically with milling. According to the results it is proposed to mill the sample between 200 and 500 μm. For accurate determination of dietary fibre content, it is necessary to prepare the sample correctly, since the AOAC 991.43 method is, despite its robustness, sensitive during the sample preparation step.
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publishDate 2018-04-01
publisher University of Ljubljana Press (Založba Univerze v Ljubljani)
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spelling doaj-art-5ca3bd49ade64f5f8d52545818a23a8f2025-08-20T02:58:55ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412018-04-01111111111910.14720/aas.2018.111.1.1119156DETERMINATION OF DIETARY FIBRE – THE INFLUENCE OF MILLING FRACTION AND MIXING PROCESSBlaž FERJANČIČ0Jasna BERTONCELJ1University of Ljubljana, Biotechnical Faculty, Department of Food Science and Technololy SloveniaUniversity of Ljubljana, Biotechnical Faculty, Department of Food Science and Technololy SloveniaDietary fibre is an important constituent of a healthy diet, composed of non-digestible carbohydrates and lignin. Over the last decades dietary fibre has gained importance for human nutrition, due to its beneficial effects on health. In addition to classical enzyme-gravimetric methods, new methods for the determination of total, insoluble and soluble dietary fibres in foods have recently been developed, but have not yet been fully implemented for use. For the purpose of creating food composition databases and for food labelling, the classical AOAC 985.29 and 991.43 methods are still widely used. The methods are enzyme-gravimetric and therefore sensitive to enzyme kinetics. The aim of the study was to investigate the effect of milling fraction and mixing of the sample on dietary fibre content determined with the AOAC method 991.43. The results showed that milling fraction significantly influences the content of dietary fibre, especially in unprocessed or slightly processed cereals, the mixing acts synergistically with milling. According to the results it is proposed to mill the sample between 200 and 500 μm. For accurate determination of dietary fibre content, it is necessary to prepare the sample correctly, since the AOAC 991.43 method is, despite its robustness, sensitive during the sample preparation step.https://journals.uni-lj.si/aas/article/view/12764dietary fibreaoac official methodsbreakfast cerealsmillingfractionationmixing
spellingShingle Blaž FERJANČIČ
Jasna BERTONCELJ
DETERMINATION OF DIETARY FIBRE – THE INFLUENCE OF MILLING FRACTION AND MIXING PROCESS
Acta Agriculturae Slovenica
dietary fibre
aoac official methods
breakfast cereals
milling
fractionation
mixing
title DETERMINATION OF DIETARY FIBRE – THE INFLUENCE OF MILLING FRACTION AND MIXING PROCESS
title_full DETERMINATION OF DIETARY FIBRE – THE INFLUENCE OF MILLING FRACTION AND MIXING PROCESS
title_fullStr DETERMINATION OF DIETARY FIBRE – THE INFLUENCE OF MILLING FRACTION AND MIXING PROCESS
title_full_unstemmed DETERMINATION OF DIETARY FIBRE – THE INFLUENCE OF MILLING FRACTION AND MIXING PROCESS
title_short DETERMINATION OF DIETARY FIBRE – THE INFLUENCE OF MILLING FRACTION AND MIXING PROCESS
title_sort determination of dietary fibre the influence of milling fraction and mixing process
topic dietary fibre
aoac official methods
breakfast cereals
milling
fractionation
mixing
url https://journals.uni-lj.si/aas/article/view/12764
work_keys_str_mv AT blazferjancic determinationofdietaryfibretheinfluenceofmillingfractionandmixingprocess
AT jasnabertoncelj determinationofdietaryfibretheinfluenceofmillingfractionandmixingprocess