Population Genetic Analysis of Lobelia rhynchopetalum Hemsl. (Campanulaceae) Using DNA Sequences from ITS and Eight Chloroplast DNA Regions
DNA sequence data from the internal transcribed spacer of nuclear ribosomal DNA and eight chloroplast DNA regions were used to investigate haplotypic variation and population genetic structure of the Afroalpine giant lobelia, Lobelia rhynchopetalum. The study was based on eight populations sampled f...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/276451 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832554420869529600 |
|---|---|
| author | Mulatu Geleta Tomas Bryngelsson |
| author_facet | Mulatu Geleta Tomas Bryngelsson |
| author_sort | Mulatu Geleta |
| collection | DOAJ |
| description | DNA sequence data from the internal transcribed spacer of nuclear ribosomal DNA and eight chloroplast DNA regions were used to investigate haplotypic variation and population genetic structure of the Afroalpine giant lobelia, Lobelia rhynchopetalum. The study was based on eight populations sampled from two mountain systems in Ethiopia. A total of 20 variable sites were obtained, which resulted in 13 unique haplotypes and an overall nucleotide diversity (ND) of 0.281 ± 0.15 and gene diversity (GD) of 0.85 ± 0.04. Analysis of molecular variance (AMOVA) revealed a highly significant variation (P<0.001) among populations (FST), and phylogenetic analysis revealed that populations from the two mountain systems formed their own distinct clade with >90% bootstrap support. Each population should be regarded as a significant unit for conservation of this species. The primers designed for this study can be applied to any Lobelia and other closely related species for population genetics and phylogenetic studies. |
| format | Article |
| id | doaj-art-5c97f70ce2924406ab2f41e11d464b57 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-5c97f70ce2924406ab2f41e11d464b572025-02-03T05:51:31ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/276451276451Population Genetic Analysis of Lobelia rhynchopetalum Hemsl. (Campanulaceae) Using DNA Sequences from ITS and Eight Chloroplast DNA RegionsMulatu Geleta0Tomas Bryngelsson1Department of Plant Breeding and Biotechnology, Swedish University of Agricultural Sciences, P.O. Box 101, 230 53 Alnarp, SwedenDepartment of Plant Breeding and Biotechnology, Swedish University of Agricultural Sciences, P.O. Box 101, 230 53 Alnarp, SwedenDNA sequence data from the internal transcribed spacer of nuclear ribosomal DNA and eight chloroplast DNA regions were used to investigate haplotypic variation and population genetic structure of the Afroalpine giant lobelia, Lobelia rhynchopetalum. The study was based on eight populations sampled from two mountain systems in Ethiopia. A total of 20 variable sites were obtained, which resulted in 13 unique haplotypes and an overall nucleotide diversity (ND) of 0.281 ± 0.15 and gene diversity (GD) of 0.85 ± 0.04. Analysis of molecular variance (AMOVA) revealed a highly significant variation (P<0.001) among populations (FST), and phylogenetic analysis revealed that populations from the two mountain systems formed their own distinct clade with >90% bootstrap support. Each population should be regarded as a significant unit for conservation of this species. The primers designed for this study can be applied to any Lobelia and other closely related species for population genetics and phylogenetic studies.http://dx.doi.org/10.1100/2012/276451 |
| spellingShingle | Mulatu Geleta Tomas Bryngelsson Population Genetic Analysis of Lobelia rhynchopetalum Hemsl. (Campanulaceae) Using DNA Sequences from ITS and Eight Chloroplast DNA Regions The Scientific World Journal |
| title | Population Genetic Analysis of Lobelia rhynchopetalum Hemsl. (Campanulaceae) Using DNA Sequences from ITS and Eight Chloroplast DNA Regions |
| title_full | Population Genetic Analysis of Lobelia rhynchopetalum Hemsl. (Campanulaceae) Using DNA Sequences from ITS and Eight Chloroplast DNA Regions |
| title_fullStr | Population Genetic Analysis of Lobelia rhynchopetalum Hemsl. (Campanulaceae) Using DNA Sequences from ITS and Eight Chloroplast DNA Regions |
| title_full_unstemmed | Population Genetic Analysis of Lobelia rhynchopetalum Hemsl. (Campanulaceae) Using DNA Sequences from ITS and Eight Chloroplast DNA Regions |
| title_short | Population Genetic Analysis of Lobelia rhynchopetalum Hemsl. (Campanulaceae) Using DNA Sequences from ITS and Eight Chloroplast DNA Regions |
| title_sort | population genetic analysis of lobelia rhynchopetalum hemsl campanulaceae using dna sequences from its and eight chloroplast dna regions |
| url | http://dx.doi.org/10.1100/2012/276451 |
| work_keys_str_mv | AT mulatugeleta populationgeneticanalysisoflobeliarhynchopetalumhemslcampanulaceaeusingdnasequencesfromitsandeightchloroplastdnaregions AT tomasbryngelsson populationgeneticanalysisoflobeliarhynchopetalumhemslcampanulaceaeusingdnasequencesfromitsandeightchloroplastdnaregions |