Importance of the profile of inflammatory and anti-inflammatory cytokines in patients with Hepatitis C according to degrees of fibrosis.
Introduction and Objectives: The cure for chronic Hepatitis C (HCV) is a milestone for humanity, however, patients could still progress toward hepatocellular carcinoma even after receiving direct-acting antiviral treatment [1]. Therefore, determining the inflammatory (IFN-γ, TNF-α), and anti-inflamm...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-04-01
|
| Series: | Annals of Hepatology |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1665268125000821 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850195277100613632 |
|---|---|
| author | Abigail Hernandez-Barragan Moisés Martínez-Castillo Adrián Flores-Sánchez Jesús Dorantes-Alvarez Isabel Villagómez-López Marisela Hernandez-Santillan Andrea Garcia-Avalos José Luis Pérez-Hernández Fátima Higuera-De la Tijera Paula Cordero-Pérez Linda Muñoz-Espinosa Gabriela Gutierrez-Reyes |
| author_facet | Abigail Hernandez-Barragan Moisés Martínez-Castillo Adrián Flores-Sánchez Jesús Dorantes-Alvarez Isabel Villagómez-López Marisela Hernandez-Santillan Andrea Garcia-Avalos José Luis Pérez-Hernández Fátima Higuera-De la Tijera Paula Cordero-Pérez Linda Muñoz-Espinosa Gabriela Gutierrez-Reyes |
| author_sort | Abigail Hernandez-Barragan |
| collection | DOAJ |
| description | Introduction and Objectives: The cure for chronic Hepatitis C (HCV) is a milestone for humanity, however, patients could still progress toward hepatocellular carcinoma even after receiving direct-acting antiviral treatment [1]. Therefore, determining the inflammatory (IFN-γ, TNF-α), and anti-inflammatory (IL-10- IL-1RA) profiles in patients with different degree of liver fibrosis are the important aim in researching of new biomarkers. Materials and Methods: Prospective, cross-sectional and multicenter study; approved through the ethics committee of the UNAM, and the Hospital General de Mexico, patients with chronic Hepatitis C (CHC), and healthy subjects (control group) were included. A personalized survey of chronic and recent pathologies, as well as alcohol consumption, was performed in all the subjects. Biochemical and hematological laboratory test were performed, including Fibroscan and/or Fibrotest to determinate the degree of fibrosis. In addition, the cytokine profile (IFN-γ, TNF-α, IL-10 and IL-1RA) was quantified in the serum by multiple suspension array. The data was analyzed by Kruskal-Wallis, Mann-Whitney U and ANOVA statistical tests by SPSS v.22 software, considering p > 0.05 Results: A total of 180 CT subjects were included; whereas 90 patients with CHC with different grades of fibrosis: F0=20, F1=15, F2=15, F3=16 and F4=24, were enrolled. As has been studied, Hepatitis C Virus inhibits the activity of IFN-γ, despite of this, its concentration has been controversial in different populations. In our population, the levels were low with the exception for F2 (p<0.01). Another important factor of inflammation is TNF-α, which was found increased in all stages: F0-F4 and in F2 up to 6 times more than the CT and F4 groups (p<0.001). When analyzing the results of anti-inflammatory cytokines; the highest concentration of IL-10 was observed in F2 (increased up to 7 times) (p<0.001), while for IL-1RA in F2 it was found to be increased up to 10 times more than in F1 and F3 (p<0.05); what it shows that in F2 the inflammatory/anti-inflammatory microenvironment at the peripheral level has more activity. On the other hand, the highest concentration of IL-1RA was observed in F4, which may be actively participating in the repair due to decreased mediators of inflammation. Conclusions: The F2 stage is an important turning point, indicating a shift in the microenvironment due to the accumulation of the extracellular matrix, making it clear the importance of measuring inflammation at intermediate stages of fibrosis, which would allow for impacting the limitation of the hepatic fibrogenic process. |
| format | Article |
| id | doaj-art-5c854773e5804fe58d633468cdce71c8 |
| institution | OA Journals |
| issn | 1665-2681 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Annals of Hepatology |
| spelling | doaj-art-5c854773e5804fe58d633468cdce71c82025-08-20T02:13:48ZengElsevierAnnals of Hepatology1665-26812025-04-013010185810.1016/j.aohep.2025.101858Importance of the profile of inflammatory and anti-inflammatory cytokines in patients with Hepatitis C according to degrees of fibrosis.Abigail Hernandez-Barragan0Moisés Martínez-Castillo1Adrián Flores-Sánchez2Jesús Dorantes-Alvarez3Isabel Villagómez-López4Marisela Hernandez-Santillan5Andrea Garcia-Avalos6José Luis Pérez-Hernández7Fátima Higuera-De la Tijera8Paula Cordero-Pérez9Linda Muñoz-Espinosa10Gabriela Gutierrez-Reyes11Liver, Pancreas, and Motility Laboratory (HIPAM), Experimental Medicine Research Unit, Faculty of Medicine, UNAM, General Hospital of Mexico, Dr. Eduardo Liceaga, MexicoLiver, Pancreas, and Motility Laboratory (HIPAM), Experimental Medicine Research Unit, Faculty of Medicine, UNAM, General Hospital of Mexico, Dr. Eduardo Liceaga, MexicoLiver, Pancreas, and Motility Laboratory (HIPAM), Experimental Medicine Research Unit, Faculty of Medicine, UNAM, General Hospital of Mexico, Dr. Eduardo Liceaga, MexicoLiver, Pancreas, and Motility Laboratory (HIPAM), Experimental Medicine Research Unit, Faculty of Medicine, UNAM, General Hospital of Mexico, Dr. Eduardo Liceaga, MexicoLiver, Pancreas, and Motility Laboratory (HIPAM), Experimental Medicine Research Unit, Faculty of Medicine, UNAM, General Hospital of Mexico, Dr. Eduardo Liceaga, MexicoLiver, Pancreas, and Motility Laboratory (HIPAM), Experimental Medicine Research Unit, Faculty of Medicine, UNAM, General Hospital of Mexico, Dr. Eduardo Liceaga, MexicoLiver, Pancreas, and Motility Laboratory (HIPAM), Experimental Medicine Research Unit, Faculty of Medicine, UNAM, General Hospital of Mexico, Dr. Eduardo Liceaga, MexicoGastroenterology Department, General Hospital og México “Dr. Eduardo Liceaga” Mexico City, MéxicoGastroenterology Department, General Hospital og México “Dr. Eduardo Liceaga” Mexico City, MéxicoUniversity Hospital “Dr. José Eleuterio González”, Medicine Faculty, UANL, MexicoUniversity Hospital “Dr. José Eleuterio González”, Medicine Faculty, UANL, MexicoLiver, Pancreas, and Motility Laboratory (HIPAM), Experimental Medicine Research Unit, Faculty of Medicine, UNAM, General Hospital of Mexico, Dr. Eduardo Liceaga, MexicoIntroduction and Objectives: The cure for chronic Hepatitis C (HCV) is a milestone for humanity, however, patients could still progress toward hepatocellular carcinoma even after receiving direct-acting antiviral treatment [1]. Therefore, determining the inflammatory (IFN-γ, TNF-α), and anti-inflammatory (IL-10- IL-1RA) profiles in patients with different degree of liver fibrosis are the important aim in researching of new biomarkers. Materials and Methods: Prospective, cross-sectional and multicenter study; approved through the ethics committee of the UNAM, and the Hospital General de Mexico, patients with chronic Hepatitis C (CHC), and healthy subjects (control group) were included. A personalized survey of chronic and recent pathologies, as well as alcohol consumption, was performed in all the subjects. Biochemical and hematological laboratory test were performed, including Fibroscan and/or Fibrotest to determinate the degree of fibrosis. In addition, the cytokine profile (IFN-γ, TNF-α, IL-10 and IL-1RA) was quantified in the serum by multiple suspension array. The data was analyzed by Kruskal-Wallis, Mann-Whitney U and ANOVA statistical tests by SPSS v.22 software, considering p > 0.05 Results: A total of 180 CT subjects were included; whereas 90 patients with CHC with different grades of fibrosis: F0=20, F1=15, F2=15, F3=16 and F4=24, were enrolled. As has been studied, Hepatitis C Virus inhibits the activity of IFN-γ, despite of this, its concentration has been controversial in different populations. In our population, the levels were low with the exception for F2 (p<0.01). Another important factor of inflammation is TNF-α, which was found increased in all stages: F0-F4 and in F2 up to 6 times more than the CT and F4 groups (p<0.001). When analyzing the results of anti-inflammatory cytokines; the highest concentration of IL-10 was observed in F2 (increased up to 7 times) (p<0.001), while for IL-1RA in F2 it was found to be increased up to 10 times more than in F1 and F3 (p<0.05); what it shows that in F2 the inflammatory/anti-inflammatory microenvironment at the peripheral level has more activity. On the other hand, the highest concentration of IL-1RA was observed in F4, which may be actively participating in the repair due to decreased mediators of inflammation. Conclusions: The F2 stage is an important turning point, indicating a shift in the microenvironment due to the accumulation of the extracellular matrix, making it clear the importance of measuring inflammation at intermediate stages of fibrosis, which would allow for impacting the limitation of the hepatic fibrogenic process.http://www.sciencedirect.com/science/article/pii/S1665268125000821 |
| spellingShingle | Abigail Hernandez-Barragan Moisés Martínez-Castillo Adrián Flores-Sánchez Jesús Dorantes-Alvarez Isabel Villagómez-López Marisela Hernandez-Santillan Andrea Garcia-Avalos José Luis Pérez-Hernández Fátima Higuera-De la Tijera Paula Cordero-Pérez Linda Muñoz-Espinosa Gabriela Gutierrez-Reyes Importance of the profile of inflammatory and anti-inflammatory cytokines in patients with Hepatitis C according to degrees of fibrosis. Annals of Hepatology |
| title | Importance of the profile of inflammatory and anti-inflammatory cytokines in patients with Hepatitis C according to degrees of fibrosis. |
| title_full | Importance of the profile of inflammatory and anti-inflammatory cytokines in patients with Hepatitis C according to degrees of fibrosis. |
| title_fullStr | Importance of the profile of inflammatory and anti-inflammatory cytokines in patients with Hepatitis C according to degrees of fibrosis. |
| title_full_unstemmed | Importance of the profile of inflammatory and anti-inflammatory cytokines in patients with Hepatitis C according to degrees of fibrosis. |
| title_short | Importance of the profile of inflammatory and anti-inflammatory cytokines in patients with Hepatitis C according to degrees of fibrosis. |
| title_sort | importance of the profile of inflammatory and anti inflammatory cytokines in patients with hepatitis c according to degrees of fibrosis |
| url | http://www.sciencedirect.com/science/article/pii/S1665268125000821 |
| work_keys_str_mv | AT abigailhernandezbarragan importanceoftheprofileofinflammatoryandantiinflammatorycytokinesinpatientswithhepatitiscaccordingtodegreesoffibrosis AT moisesmartinezcastillo importanceoftheprofileofinflammatoryandantiinflammatorycytokinesinpatientswithhepatitiscaccordingtodegreesoffibrosis AT adrianfloressanchez importanceoftheprofileofinflammatoryandantiinflammatorycytokinesinpatientswithhepatitiscaccordingtodegreesoffibrosis AT jesusdorantesalvarez importanceoftheprofileofinflammatoryandantiinflammatorycytokinesinpatientswithhepatitiscaccordingtodegreesoffibrosis AT isabelvillagomezlopez importanceoftheprofileofinflammatoryandantiinflammatorycytokinesinpatientswithhepatitiscaccordingtodegreesoffibrosis AT mariselahernandezsantillan importanceoftheprofileofinflammatoryandantiinflammatorycytokinesinpatientswithhepatitiscaccordingtodegreesoffibrosis AT andreagarciaavalos importanceoftheprofileofinflammatoryandantiinflammatorycytokinesinpatientswithhepatitiscaccordingtodegreesoffibrosis AT joseluisperezhernandez importanceoftheprofileofinflammatoryandantiinflammatorycytokinesinpatientswithhepatitiscaccordingtodegreesoffibrosis AT fatimahigueradelatijera importanceoftheprofileofinflammatoryandantiinflammatorycytokinesinpatientswithhepatitiscaccordingtodegreesoffibrosis AT paulacorderoperez importanceoftheprofileofinflammatoryandantiinflammatorycytokinesinpatientswithhepatitiscaccordingtodegreesoffibrosis AT lindamunozespinosa importanceoftheprofileofinflammatoryandantiinflammatorycytokinesinpatientswithhepatitiscaccordingtodegreesoffibrosis AT gabrielagutierrezreyes importanceoftheprofileofinflammatoryandantiinflammatorycytokinesinpatientswithhepatitiscaccordingtodegreesoffibrosis |