Application of Liquid Chromatography/Tandem Mass Spectrometry for Quantitative Analysis of Plasmalogens in Preadolescent Children—The Hokkaido Study
<b>Background</b>: Plasmalogens (Pls) are phospholipids with a unique structure, abundant in the brain and heart. Due to their chemical instability and analytical difficulties, less information is available compared to other phospholipids. The importance of Pls in several cellular proces...
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2025-03-01
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| author | Yifan Chen Siddabasave Gowda B. Gowda Divyavani Gowda Jayashankar Jayaprakash Lipsa Rani Nath Atusko Ikeda Yu Ait Bamai Rahel Mesfin Ketema Reiko Kishi Hitoshi Chiba Shu-Ping Hui |
| author_facet | Yifan Chen Siddabasave Gowda B. Gowda Divyavani Gowda Jayashankar Jayaprakash Lipsa Rani Nath Atusko Ikeda Yu Ait Bamai Rahel Mesfin Ketema Reiko Kishi Hitoshi Chiba Shu-Ping Hui |
| author_sort | Yifan Chen |
| collection | DOAJ |
| description | <b>Background</b>: Plasmalogens (Pls) are phospholipids with a unique structure, abundant in the brain and heart. Due to their chemical instability and analytical difficulties, less information is available compared to other phospholipids. The importance of Pls in several cellular processes is known, one of which is their protective effect against oxidative damage. The physiological role of Pls in human development has not been elucidated. Despite their clinical importance, the quantitative analysis of Pls in children’s plasma has been limited. <b>Methods</b>: This study aims to determine the plasma levels of Pls in prepubertal children using liquid chromatography/tandem mass spectrometry (LC-MS/MS). The plasma samples used were obtained from 9- to 12-year-old girls (n = 156) and boys (n = 178), n = 334 in total, who participated in the Hokkaido study. <b>Results</b>: Ethanolamine plasmalogen (PlsEtn) and choline plasmalogen (PlsCho), both carrying eicosapentaenoic acid, were significantly lower in girls than in boys. In both sexes, the plasmalogen levels for the 12-year-old children were lower than those for the 9-year-old children. PlsCho (16:0/18:2) was lower in the overweight children than in the normal-weight children for both sexes. PlsEtn (18:0/20:4) was the most abundant ethanolamine-type plasmalogen in both sexes. <b>Conclusions</b>: This study is the first report on plasmalogen levels and molecular types in children’s plasma. This study provides the information needed to understand the role of Pls in human developmental processes and may open up new opportunities in the future to control age-related changes in Pls. |
| format | Article |
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| institution | OA Journals |
| issn | 2075-4418 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
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| series | Diagnostics |
| spelling | doaj-art-5c83dd4129f1472e8accedb1d98e3d0a2025-08-20T02:11:13ZengMDPI AGDiagnostics2075-44182025-03-0115674310.3390/diagnostics15060743Application of Liquid Chromatography/Tandem Mass Spectrometry for Quantitative Analysis of Plasmalogens in Preadolescent Children—The Hokkaido StudyYifan Chen0Siddabasave Gowda B. Gowda1Divyavani Gowda2Jayashankar Jayaprakash3Lipsa Rani Nath4Atusko Ikeda5Yu Ait Bamai6Rahel Mesfin Ketema7Reiko Kishi8Hitoshi Chiba9Shu-Ping Hui10Faculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita-ku, Sapporo 060-0812, JapanFaculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita-ku, Sapporo 060-0812, JapanFaculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita-ku, Sapporo 060-0812, JapanGraduate School of Global Food Resources, Hokkaido University, Kita-9, Nishi-9, Kita-ku, Sapporo 060-0809, JapanGraduate School of Global Food Resources, Hokkaido University, Kita-9, Nishi-9, Kita-ku, Sapporo 060-0809, JapanFaculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita-ku, Sapporo 060-0812, JapanCenter for Environmental and Health Sciences, Hokkaido University, Kita-12, Nishi-7, Kita-ku, Sapporo 060-0812, JapanFaculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita-ku, Sapporo 060-0812, JapanCenter for Environmental and Health Sciences, Hokkaido University, Kita-12, Nishi-7, Kita-ku, Sapporo 060-0812, JapanDepartment of Nutrition, Sapporo University of Health Sciences, Nakanuma Nishi-4-2-1-15, Higashi-ku, Sapporo 070-0894, JapanFaculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita-ku, Sapporo 060-0812, Japan<b>Background</b>: Plasmalogens (Pls) are phospholipids with a unique structure, abundant in the brain and heart. Due to their chemical instability and analytical difficulties, less information is available compared to other phospholipids. The importance of Pls in several cellular processes is known, one of which is their protective effect against oxidative damage. The physiological role of Pls in human development has not been elucidated. Despite their clinical importance, the quantitative analysis of Pls in children’s plasma has been limited. <b>Methods</b>: This study aims to determine the plasma levels of Pls in prepubertal children using liquid chromatography/tandem mass spectrometry (LC-MS/MS). The plasma samples used were obtained from 9- to 12-year-old girls (n = 156) and boys (n = 178), n = 334 in total, who participated in the Hokkaido study. <b>Results</b>: Ethanolamine plasmalogen (PlsEtn) and choline plasmalogen (PlsCho), both carrying eicosapentaenoic acid, were significantly lower in girls than in boys. In both sexes, the plasmalogen levels for the 12-year-old children were lower than those for the 9-year-old children. PlsCho (16:0/18:2) was lower in the overweight children than in the normal-weight children for both sexes. PlsEtn (18:0/20:4) was the most abundant ethanolamine-type plasmalogen in both sexes. <b>Conclusions</b>: This study is the first report on plasmalogen levels and molecular types in children’s plasma. This study provides the information needed to understand the role of Pls in human developmental processes and may open up new opportunities in the future to control age-related changes in Pls.https://www.mdpi.com/2075-4418/15/6/743plasmalogensliquid chromatographymass spectrometrychildren’s plasmaoverweight |
| spellingShingle | Yifan Chen Siddabasave Gowda B. Gowda Divyavani Gowda Jayashankar Jayaprakash Lipsa Rani Nath Atusko Ikeda Yu Ait Bamai Rahel Mesfin Ketema Reiko Kishi Hitoshi Chiba Shu-Ping Hui Application of Liquid Chromatography/Tandem Mass Spectrometry for Quantitative Analysis of Plasmalogens in Preadolescent Children—The Hokkaido Study Diagnostics plasmalogens liquid chromatography mass spectrometry children’s plasma overweight |
| title | Application of Liquid Chromatography/Tandem Mass Spectrometry for Quantitative Analysis of Plasmalogens in Preadolescent Children—The Hokkaido Study |
| title_full | Application of Liquid Chromatography/Tandem Mass Spectrometry for Quantitative Analysis of Plasmalogens in Preadolescent Children—The Hokkaido Study |
| title_fullStr | Application of Liquid Chromatography/Tandem Mass Spectrometry for Quantitative Analysis of Plasmalogens in Preadolescent Children—The Hokkaido Study |
| title_full_unstemmed | Application of Liquid Chromatography/Tandem Mass Spectrometry for Quantitative Analysis of Plasmalogens in Preadolescent Children—The Hokkaido Study |
| title_short | Application of Liquid Chromatography/Tandem Mass Spectrometry for Quantitative Analysis of Plasmalogens in Preadolescent Children—The Hokkaido Study |
| title_sort | application of liquid chromatography tandem mass spectrometry for quantitative analysis of plasmalogens in preadolescent children the hokkaido study |
| topic | plasmalogens liquid chromatography mass spectrometry children’s plasma overweight |
| url | https://www.mdpi.com/2075-4418/15/6/743 |
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