Breeding of highly efficient detoxification strains of cottonseed meal
The aim was to improve and screen the strains with high free gossypol (FG) degradation rate. With Candidaoleophila, Kloeckera lindneri, Rhodothece glutinis as the starting strains, the mutants with high free gossypol (FG) degradation rate were bred after the starting strains were treated by ultravio...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2011-05-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2011.03.003 |
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| Summary: | The aim was to improve and screen the strains with high free gossypol (FG) degradation rate. With Candidaoleophila, Kloeckera lindneri, Rhodothece glutinis as the starting strains, the mutants with high free gossypol (FG) degradation rate were bred after the starting strains were treated by ultraviolet (UV), diethyl sulfate (DES) and UV + DES mutation. And the optimal conditions for mutation were confirmed. The results showed that six available mutant strains were screened through primary screening and secondary screening (GJZ-25, GJ-D-25, GJ-Z-25-D-25, GJ-Z-20-D-20, HJ-D-20, HJ-Z-20-D-20). The FG degradation rate of mutant strains were 90.69%, 91.40%, 93.78%, 89.64%, 90.78% and 89.56% respectively, increased by 50.00%, 51.20%, 55.14%, 48.29%, 26.33% and 24.63%, compared with the starting strains. The composite mutation (UV irradiation mutagenesis of 25 s, DES treatment of 25 min) of C. oleophila was more effective than alone mutation; but alone DES mutagenesis of R. glutinis was more effective than the compound mutation, and the best condition for DES mutagenesis treatment was for 20 min. The experiment obtained high concentrations gossypol tolerated strains, enhanced the degradation rate of gossypol significantly and increased nutritional value of cottonseed meal. |
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| ISSN: | 1008-9209 2097-5155 |