Combined Effects of Polyethylene and Bordeaux Mixture on the Soil–Plant System: Phytotoxicity, Copper Accumulation and Changes in Microbial Abundance
Greenhouses have positively impacted plant production by allowing the cultivation of different crops per year. However, the accumulation of agricultural plastics, potentially contaminated with agrochemicals, raises environmental concerns. This work evaluates the combined effect of Bordeaux mixture a...
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2025-08-01
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| author | Silvia Romeo-Río Huguette Meta Foguieng Antía Gómez-Armesto Manuel Conde-Cid David Fernández-Calviño Andrés Rodríguez-Seijo |
| author_facet | Silvia Romeo-Río Huguette Meta Foguieng Antía Gómez-Armesto Manuel Conde-Cid David Fernández-Calviño Andrés Rodríguez-Seijo |
| author_sort | Silvia Romeo-Río |
| collection | DOAJ |
| description | Greenhouses have positively impacted plant production by allowing the cultivation of different crops per year. However, the accumulation of agricultural plastics, potentially contaminated with agrochemicals, raises environmental concerns. This work evaluates the combined effect of Bordeaux mixture and low-density polyethylene (LDPE) microplastics (<5 mm) on the growth of lettuce (<i>Lactuca sativa</i> L.) and soil microbial communities. Different levels of Bordeaux mixture (0, 100 and 500 mg kg<sup>−1</sup>), equivalent to Cu(II) concentrations (0, 17 and 83 mg kg<sup>−1</sup>), LDPE microplastics (0, 1% and 5%) and their combination were selected. After 28 days of growth, biometric and photosynthetic parameters, Cu uptake, and soil microbial responses were evaluated. Plant germination and growth were not significantly affected by the combination of Cu and plastics. However, individual Cu treatments influenced root and shoot length and biomass. Chlorophyll and carotenoid concentrations increased with Cu addition, although the differences were not statistically significant. Phospholipid fatty acid (PLFA) analysis revealed a reduction in microbial biomass at the highest Cu dose, whereas LDPE alone showed limited effects and may reduce Cu bioavailability. These results suggest that even at the highest concentration added, Cu can act as a plant nutrient, while the combination of Cu–plastics showed varying effects on plant growth and soil microbial communities. |
| format | Article |
| id | doaj-art-5c744f9de50d45c898b3ddd6470cad74 |
| institution | DOAJ |
| issn | 2077-0472 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | MDPI AG |
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| series | Agriculture |
| spelling | doaj-art-5c744f9de50d45c898b3ddd6470cad742025-08-20T03:04:40ZengMDPI AGAgriculture2077-04722025-08-011515165710.3390/agriculture15151657Combined Effects of Polyethylene and Bordeaux Mixture on the Soil–Plant System: Phytotoxicity, Copper Accumulation and Changes in Microbial AbundanceSilvia Romeo-Río0Huguette Meta Foguieng1Antía Gómez-Armesto2Manuel Conde-Cid3David Fernández-Calviño4Andrés Rodríguez-Seijo5Área de Edafoloxía e Química Agrícola, Departamento de Bioloxía Vexetal e Ciencia do Solo, Facultade de Ciencias, Universidade de Vigo, As Lagoas s/n, 32004 Ourense, SpainInstituto de Agroecoloxía e Alimentación (IAA), Universidade de Vigo, Campus Auga, 32004 Ourense, SpainÁrea de Edafoloxía e Química Agrícola, Departamento de Bioloxía Vexetal e Ciencia do Solo, Facultade de Ciencias, Universidade de Vigo, As Lagoas s/n, 32004 Ourense, SpainÁrea de Edafoloxía e Química Agrícola, Departamento de Bioloxía Vexetal e Ciencia do Solo, Facultade de Ciencias, Universidade de Vigo, As Lagoas s/n, 32004 Ourense, SpainÁrea de Edafoloxía e Química Agrícola, Departamento de Bioloxía Vexetal e Ciencia do Solo, Facultade de Ciencias, Universidade de Vigo, As Lagoas s/n, 32004 Ourense, SpainÁrea de Edafoloxía e Química Agrícola, Departamento de Bioloxía Vexetal e Ciencia do Solo, Facultade de Ciencias, Universidade de Vigo, As Lagoas s/n, 32004 Ourense, SpainGreenhouses have positively impacted plant production by allowing the cultivation of different crops per year. However, the accumulation of agricultural plastics, potentially contaminated with agrochemicals, raises environmental concerns. This work evaluates the combined effect of Bordeaux mixture and low-density polyethylene (LDPE) microplastics (<5 mm) on the growth of lettuce (<i>Lactuca sativa</i> L.) and soil microbial communities. Different levels of Bordeaux mixture (0, 100 and 500 mg kg<sup>−1</sup>), equivalent to Cu(II) concentrations (0, 17 and 83 mg kg<sup>−1</sup>), LDPE microplastics (0, 1% and 5%) and their combination were selected. After 28 days of growth, biometric and photosynthetic parameters, Cu uptake, and soil microbial responses were evaluated. Plant germination and growth were not significantly affected by the combination of Cu and plastics. However, individual Cu treatments influenced root and shoot length and biomass. Chlorophyll and carotenoid concentrations increased with Cu addition, although the differences were not statistically significant. Phospholipid fatty acid (PLFA) analysis revealed a reduction in microbial biomass at the highest Cu dose, whereas LDPE alone showed limited effects and may reduce Cu bioavailability. These results suggest that even at the highest concentration added, Cu can act as a plant nutrient, while the combination of Cu–plastics showed varying effects on plant growth and soil microbial communities.https://www.mdpi.com/2077-0472/15/15/1657agricultural areasecotoxicologymicroorganismsgrowth testchlorophyllcarotenoids |
| spellingShingle | Silvia Romeo-Río Huguette Meta Foguieng Antía Gómez-Armesto Manuel Conde-Cid David Fernández-Calviño Andrés Rodríguez-Seijo Combined Effects of Polyethylene and Bordeaux Mixture on the Soil–Plant System: Phytotoxicity, Copper Accumulation and Changes in Microbial Abundance Agriculture agricultural areas ecotoxicology microorganisms growth test chlorophyll carotenoids |
| title | Combined Effects of Polyethylene and Bordeaux Mixture on the Soil–Plant System: Phytotoxicity, Copper Accumulation and Changes in Microbial Abundance |
| title_full | Combined Effects of Polyethylene and Bordeaux Mixture on the Soil–Plant System: Phytotoxicity, Copper Accumulation and Changes in Microbial Abundance |
| title_fullStr | Combined Effects of Polyethylene and Bordeaux Mixture on the Soil–Plant System: Phytotoxicity, Copper Accumulation and Changes in Microbial Abundance |
| title_full_unstemmed | Combined Effects of Polyethylene and Bordeaux Mixture on the Soil–Plant System: Phytotoxicity, Copper Accumulation and Changes in Microbial Abundance |
| title_short | Combined Effects of Polyethylene and Bordeaux Mixture on the Soil–Plant System: Phytotoxicity, Copper Accumulation and Changes in Microbial Abundance |
| title_sort | combined effects of polyethylene and bordeaux mixture on the soil plant system phytotoxicity copper accumulation and changes in microbial abundance |
| topic | agricultural areas ecotoxicology microorganisms growth test chlorophyll carotenoids |
| url | https://www.mdpi.com/2077-0472/15/15/1657 |
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