Eco-Friendly Extraction of Phlorotannins from <i>Padina pavonica</i>: Identification Related to Purification Methods Towards Innovative Cosmetic Applications
This study focuses on developing innovative and eco-friendly purification methods for the isolation of bioactive compounds derived from <i>Padina pavonica</i>, a brown abundant macroalga in Djibouti. Three distinct fractions, obtained via liquid-liquid extraction (LLE_FAE), solid-phase e...
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2024-12-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/23/1/15 |
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| author | Moustapha Nour Valérie Stiger-Pouvreau Alain Guenneguez Laurence Meslet-Cladière Stéphane Cérantola Ahmed Ali Gaelle Simon Abdourahman Daher Sylvain Petek |
| author_facet | Moustapha Nour Valérie Stiger-Pouvreau Alain Guenneguez Laurence Meslet-Cladière Stéphane Cérantola Ahmed Ali Gaelle Simon Abdourahman Daher Sylvain Petek |
| author_sort | Moustapha Nour |
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| description | This study focuses on developing innovative and eco-friendly purification methods for the isolation of bioactive compounds derived from <i>Padina pavonica</i>, a brown abundant macroalga in Djibouti. Three distinct fractions, obtained via liquid-liquid extraction (LLE_FAE), solid-phase extraction (SPE_WE50), and flash chromatography (FC_EtOH20), were selected based on their high phenolic content and antioxidant activities. All fractions were also evaluated for their anti-ageing potential by assessing their ability to inhibit two vital skin-ageing enzymes, tyrosinase and elastase. Structural analysis by <sup>1</sup>H-<sup>13</sup>C HMBC NMR and LC-MS revealed a selectivity of phlorotannins depending on the purification methods. The LLE_FAE fraction exhibited greater structural complexity, including compounds such as phloroglucinol, diphlorethol/difucol, fucophlorethol and bifuhalol, which likely contribute to its enhanced bioactivity compared to the fractions obtained by FC_EtOH20 and SPE_WE50, which were also active and enriched only in phloroglucinol and fucophlorethol. These findings highlight the impact of purification techniques on the selective enrichment of specific bioactive compounds and demonstrated the interest of FC or SPE in producing active phlorotannin-enriched fractions. These two purification methods hold strong potential for innovative cosmeceutical applications. Results are discussed regarding the use of <i>P. pavonica</i> as a promising marine resource in Djibouti to be used for the development of cosmetic industry. |
| format | Article |
| id | doaj-art-5c6f897ded2146958124cb57b60a2e30 |
| institution | Kabale University |
| issn | 1660-3397 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj-art-5c6f897ded2146958124cb57b60a2e302025-01-24T13:39:28ZengMDPI AGMarine Drugs1660-33972024-12-012311510.3390/md23010015Eco-Friendly Extraction of Phlorotannins from <i>Padina pavonica</i>: Identification Related to Purification Methods Towards Innovative Cosmetic ApplicationsMoustapha Nour0Valérie Stiger-Pouvreau1Alain Guenneguez2Laurence Meslet-Cladière3Stéphane Cérantola4Ahmed Ali5Gaelle Simon6Abdourahman Daher7Sylvain Petek8Univ Brest, Institut de Recherche pour le Développement (IRD), CNRS, Ifremer, LEMAR, IUEM, F-29280 Plouzane, FranceUniv Brest, Institut de Recherche pour le Développement (IRD), CNRS, Ifremer, LEMAR, IUEM, F-29280 Plouzane, FranceUniv Brest, Institut de Recherche pour le Développement (IRD), CNRS, Ifremer, LEMAR, IUEM, F-29280 Plouzane, FranceUniv Brest, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire Universitaire de Biodiversité et Écologie Microbienne, F-29280 Plouzane, FranceUniv Brest, Plateforme Résonance Magnétique Nucléaire-Résonance Paramagnétique Electronique (RMN-RPE), F-29238 Brest, FranceCentre d’Études et de Recherche de Djibouti, Institut des Sciences de la Vie ISV, Route de l’Aéroport, Haramous BP 486, DjiboutiUniv Brest, Plateforme Résonance Magnétique Nucléaire-Résonance Paramagnétique Electronique (RMN-RPE), F-29238 Brest, FranceCentre d’Études et de Recherche de Djibouti, Institut des Sciences de la Vie ISV, Route de l’Aéroport, Haramous BP 486, DjiboutiUniv Brest, Institut de Recherche pour le Développement (IRD), CNRS, Ifremer, LEMAR, IUEM, F-29280 Plouzane, FranceThis study focuses on developing innovative and eco-friendly purification methods for the isolation of bioactive compounds derived from <i>Padina pavonica</i>, a brown abundant macroalga in Djibouti. Three distinct fractions, obtained via liquid-liquid extraction (LLE_FAE), solid-phase extraction (SPE_WE50), and flash chromatography (FC_EtOH20), were selected based on their high phenolic content and antioxidant activities. All fractions were also evaluated for their anti-ageing potential by assessing their ability to inhibit two vital skin-ageing enzymes, tyrosinase and elastase. Structural analysis by <sup>1</sup>H-<sup>13</sup>C HMBC NMR and LC-MS revealed a selectivity of phlorotannins depending on the purification methods. The LLE_FAE fraction exhibited greater structural complexity, including compounds such as phloroglucinol, diphlorethol/difucol, fucophlorethol and bifuhalol, which likely contribute to its enhanced bioactivity compared to the fractions obtained by FC_EtOH20 and SPE_WE50, which were also active and enriched only in phloroglucinol and fucophlorethol. These findings highlight the impact of purification techniques on the selective enrichment of specific bioactive compounds and demonstrated the interest of FC or SPE in producing active phlorotannin-enriched fractions. These two purification methods hold strong potential for innovative cosmeceutical applications. Results are discussed regarding the use of <i>P. pavonica</i> as a promising marine resource in Djibouti to be used for the development of cosmetic industry.https://www.mdpi.com/1660-3397/23/1/15antioxidantseco-friendly extractionphenolic compoundsLC-MSNMRcosmeceuticals |
| spellingShingle | Moustapha Nour Valérie Stiger-Pouvreau Alain Guenneguez Laurence Meslet-Cladière Stéphane Cérantola Ahmed Ali Gaelle Simon Abdourahman Daher Sylvain Petek Eco-Friendly Extraction of Phlorotannins from <i>Padina pavonica</i>: Identification Related to Purification Methods Towards Innovative Cosmetic Applications Marine Drugs antioxidants eco-friendly extraction phenolic compounds LC-MS NMR cosmeceuticals |
| title | Eco-Friendly Extraction of Phlorotannins from <i>Padina pavonica</i>: Identification Related to Purification Methods Towards Innovative Cosmetic Applications |
| title_full | Eco-Friendly Extraction of Phlorotannins from <i>Padina pavonica</i>: Identification Related to Purification Methods Towards Innovative Cosmetic Applications |
| title_fullStr | Eco-Friendly Extraction of Phlorotannins from <i>Padina pavonica</i>: Identification Related to Purification Methods Towards Innovative Cosmetic Applications |
| title_full_unstemmed | Eco-Friendly Extraction of Phlorotannins from <i>Padina pavonica</i>: Identification Related to Purification Methods Towards Innovative Cosmetic Applications |
| title_short | Eco-Friendly Extraction of Phlorotannins from <i>Padina pavonica</i>: Identification Related to Purification Methods Towards Innovative Cosmetic Applications |
| title_sort | eco friendly extraction of phlorotannins from i padina pavonica i identification related to purification methods towards innovative cosmetic applications |
| topic | antioxidants eco-friendly extraction phenolic compounds LC-MS NMR cosmeceuticals |
| url | https://www.mdpi.com/1660-3397/23/1/15 |
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