A method for the direct identification of differentiating muscle cells by a fluorescent mitochondrial dye.
Identification of differentiating muscle cells generally requires fixation, antibodies directed against muscle specific proteins, and lengthy staining processes or, alternatively, transfection of muscle specific reporter genes driving GFP expression. In this study, we examined the possibility of usi...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0028628&type=printable |
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| author | Tetsuaki Miyake John C McDermott Anthony O Gramolini |
| author_facet | Tetsuaki Miyake John C McDermott Anthony O Gramolini |
| author_sort | Tetsuaki Miyake |
| collection | DOAJ |
| description | Identification of differentiating muscle cells generally requires fixation, antibodies directed against muscle specific proteins, and lengthy staining processes or, alternatively, transfection of muscle specific reporter genes driving GFP expression. In this study, we examined the possibility of using the robust mitochondrial network seen in maturing muscle cells as a marker of cellular differentiation. The mitochondrial fluorescent tracking dye, MitoTracker, which is a cell-permeable, low toxicity, fluorescent dye, allowed us to distinguish and track living differentiating muscle cells visually by epi-fluorescence microscopy. MitoTracker staining provides a robust and simple detection strategy for living differentiating cells in culture without the need for fixation or biochemical processing. |
| format | Article |
| id | doaj-art-5c696e1ce8e640e39e538db9d193b178 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-5c696e1ce8e640e39e538db9d193b1782025-08-20T02:30:46ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-01612e2862810.1371/journal.pone.0028628A method for the direct identification of differentiating muscle cells by a fluorescent mitochondrial dye.Tetsuaki MiyakeJohn C McDermottAnthony O GramoliniIdentification of differentiating muscle cells generally requires fixation, antibodies directed against muscle specific proteins, and lengthy staining processes or, alternatively, transfection of muscle specific reporter genes driving GFP expression. In this study, we examined the possibility of using the robust mitochondrial network seen in maturing muscle cells as a marker of cellular differentiation. The mitochondrial fluorescent tracking dye, MitoTracker, which is a cell-permeable, low toxicity, fluorescent dye, allowed us to distinguish and track living differentiating muscle cells visually by epi-fluorescence microscopy. MitoTracker staining provides a robust and simple detection strategy for living differentiating cells in culture without the need for fixation or biochemical processing.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0028628&type=printable |
| spellingShingle | Tetsuaki Miyake John C McDermott Anthony O Gramolini A method for the direct identification of differentiating muscle cells by a fluorescent mitochondrial dye. PLoS ONE |
| title | A method for the direct identification of differentiating muscle cells by a fluorescent mitochondrial dye. |
| title_full | A method for the direct identification of differentiating muscle cells by a fluorescent mitochondrial dye. |
| title_fullStr | A method for the direct identification of differentiating muscle cells by a fluorescent mitochondrial dye. |
| title_full_unstemmed | A method for the direct identification of differentiating muscle cells by a fluorescent mitochondrial dye. |
| title_short | A method for the direct identification of differentiating muscle cells by a fluorescent mitochondrial dye. |
| title_sort | method for the direct identification of differentiating muscle cells by a fluorescent mitochondrial dye |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0028628&type=printable |
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