Evaluation of Two Multiplexed qPCR Assays for Malaria Detection and Speciation: A Comparative Study With Nested PCR and Microscopy
Conclusions: The qPCR kits, particularly Viasure, demonstrated superior sensitivity for detecting Plasmodium species and identifying mixed infections compared to light microscopy and NM-PCR. While light microscopy showed higher specificity and PPV, qPCR effectively detected low parasitemia levels mi...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/japr/4950793 |
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| author | Ahmed A. Muyidi Musa A. Ayashi Majed H. Wakid Maimonah S. Alghanmi Fadi M. Baakdah Hattan S. Gattan Isra M. Alsaady Muslimah N. Alsulami Haleema H. Albohiri Sarah A. Altwaim Zaki M. Eisa Thamer M. Brek |
| author_facet | Ahmed A. Muyidi Musa A. Ayashi Majed H. Wakid Maimonah S. Alghanmi Fadi M. Baakdah Hattan S. Gattan Isra M. Alsaady Muslimah N. Alsulami Haleema H. Albohiri Sarah A. Altwaim Zaki M. Eisa Thamer M. Brek |
| author_sort | Ahmed A. Muyidi |
| collection | DOAJ |
| description | Conclusions: The qPCR kits, particularly Viasure, demonstrated superior sensitivity for detecting Plasmodium species and identifying mixed infections compared to light microscopy and NM-PCR. While light microscopy showed higher specificity and PPV, qPCR effectively detected low parasitemia levels missed by microscopy, highlighting its value in improving malaria diagnostics. |
| format | Article |
| id | doaj-art-5c57da72c4a642fb80be3942a2be2f26 |
| institution | DOAJ |
| issn | 2090-0031 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-5c57da72c4a642fb80be3942a2be2f262025-08-20T03:15:26ZengWileyJournal of Parasitology Research2090-00312025-01-01202510.1155/japr/4950793Evaluation of Two Multiplexed qPCR Assays for Malaria Detection and Speciation: A Comparative Study With Nested PCR and MicroscopyAhmed A. Muyidi0Musa A. Ayashi1Majed H. Wakid2Maimonah S. Alghanmi3Fadi M. Baakdah4Hattan S. Gattan5Isra M. Alsaady6Muslimah N. Alsulami7Haleema H. Albohiri8Sarah A. Altwaim9Zaki M. Eisa10Thamer M. Brek11Main Laboratory and Blood BankMain Laboratory and Blood BankDepartment of Medical Laboratory SciencesDepartment of Medical Laboratory SciencesDepartment of Medical Laboratory SciencesDepartment of Medical Laboratory SciencesDepartment of Medical Laboratory SciencesDepartment of Biological SciencesDepartment of Biological SciencesDepartment of Clinical Microbiology and ImmunologyVector-Borne Diseases LaboratoriesPublic Health LaboratoryConclusions: The qPCR kits, particularly Viasure, demonstrated superior sensitivity for detecting Plasmodium species and identifying mixed infections compared to light microscopy and NM-PCR. While light microscopy showed higher specificity and PPV, qPCR effectively detected low parasitemia levels missed by microscopy, highlighting its value in improving malaria diagnostics.http://dx.doi.org/10.1155/japr/4950793 |
| spellingShingle | Ahmed A. Muyidi Musa A. Ayashi Majed H. Wakid Maimonah S. Alghanmi Fadi M. Baakdah Hattan S. Gattan Isra M. Alsaady Muslimah N. Alsulami Haleema H. Albohiri Sarah A. Altwaim Zaki M. Eisa Thamer M. Brek Evaluation of Two Multiplexed qPCR Assays for Malaria Detection and Speciation: A Comparative Study With Nested PCR and Microscopy Journal of Parasitology Research |
| title | Evaluation of Two Multiplexed qPCR Assays for Malaria Detection and Speciation: A Comparative Study With Nested PCR and Microscopy |
| title_full | Evaluation of Two Multiplexed qPCR Assays for Malaria Detection and Speciation: A Comparative Study With Nested PCR and Microscopy |
| title_fullStr | Evaluation of Two Multiplexed qPCR Assays for Malaria Detection and Speciation: A Comparative Study With Nested PCR and Microscopy |
| title_full_unstemmed | Evaluation of Two Multiplexed qPCR Assays for Malaria Detection and Speciation: A Comparative Study With Nested PCR and Microscopy |
| title_short | Evaluation of Two Multiplexed qPCR Assays for Malaria Detection and Speciation: A Comparative Study With Nested PCR and Microscopy |
| title_sort | evaluation of two multiplexed qpcr assays for malaria detection and speciation a comparative study with nested pcr and microscopy |
| url | http://dx.doi.org/10.1155/japr/4950793 |
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