Evaluation of Two Multiplexed qPCR Assays for Malaria Detection and Speciation: A Comparative Study With Nested PCR and Microscopy

Evaluation of Two Multiplexed qPCR Assays for Malaria Detection and Speciation: A Comparative Study With Nested PCR and Microscopy

Conclusions: The qPCR kits, particularly Viasure, demonstrated superior sensitivity for detecting Plasmodium species and identifying mixed infections compared to light microscopy and NM-PCR. While light microscopy showed higher specificity and PPV, qPCR effectively detected low parasitemia levels mi...

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Main Authors: Ahmed A. Muyidi, Musa A. Ayashi, Majed H. Wakid, Maimonah S. Alghanmi, Fadi M. Baakdah, Hattan S. Gattan, Isra M. Alsaady, Muslimah N. Alsulami, Haleema H. Albohiri, Sarah A. Altwaim, Zaki M. Eisa, Thamer M. Brek
Format: Article
Language:English
Published: Wiley 2025-01-01
Series:Journal of Parasitology Research
Online Access:http://dx.doi.org/10.1155/japr/4950793
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Summary:Conclusions: The qPCR kits, particularly Viasure, demonstrated superior sensitivity for detecting Plasmodium species and identifying mixed infections compared to light microscopy and NM-PCR. While light microscopy showed higher specificity and PPV, qPCR effectively detected low parasitemia levels missed by microscopy, highlighting its value in improving malaria diagnostics.
ISSN:2090-0031

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