Inoculant Formulation for <i>Bradyrhizobium</i> spp.: Optimizing CMC/Starch Blends for Improved Performance

Inoculant Formulation for <i>Bradyrhizobium</i> spp.: Optimizing CMC/Starch Blends for Improved Performance

Inoculating legumes with nitrogen-fixing bacteria, such as <i>Bradyrhizobium</i>, can significantly reduce reliance on synthetic nitrogen fertilizers. To optimize this process, a suitable rhizobial strain must be carefully selected and formulated. This study aimed to develop a biopolymer...

Full description

Saved in:
Bibliographic Details
Main Authors: Jaqueline Carvalho de Almeida, Vinício Oliosi Favero, Janaina Ribeiro Costa Rouws, Carla de Sant’Anna Freitas, Érica Barbosa de Sousa, Jéssica Feitoza da Rocha, Nainicelle Cibelle Sousa Chantre, Gustavo Ribeiro Xavier, Paulo Jansen de Oliveira, Norma Gouvêa Rumjanek
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Agriculture
Subjects:
biological nitrogen fixation
polymeric mixture carrier
biofertilizer
Online Access:https://www.mdpi.com/2077-0472/15/9/1010
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Inoculating legumes with nitrogen-fixing bacteria, such as <i>Bradyrhizobium</i>, can significantly reduce reliance on synthetic nitrogen fertilizers. To optimize this process, a suitable rhizobial strain must be carefully selected and formulated. This study aimed to develop a biopolymer blend formulation for <i>Bradyrhizobium pachyrhizi</i> strain BR 3262. From four commercial starches and two carboxymethylcelluloses (CMC), we developed CMC/starch blends compatibilized or not with MgO at concentrations from 0.1% to 1.0% and subjected them to autoclaving for either 30 or 60 min. The resulting inoculants were stored for 168 days. Generally, blends compatibilized with 1.0% MgO exhibited a significant decrease in cell numbers, likely due to the observed pH values of approximately 10. The best performance was observed for CMC-I/starch B blends autoclaved for 60 min, and CMC-II/starch C blends autoclaved for 30 min, both compatibilized with 0.3% MgO. These blends maintained a cell viability of 10<sup>8</sup> CFU mL<sup>−1</sup> for approximately 130 days at room temperature. Blend optimization depends on the selection of specific interactions and quantities of each component in order to achieve a given functionality; in the conditions of this study, the capacity to maintain <i>Bradyrhizobium</i> cell viability for at least four months.
ISSN:2077-0472

Similar Items