Repeatability of Perimacular Ganglion Cell Complex Analysis with Spectral-Domain Optical Coherence Tomography

Purpose. To assess the repeatability of spectral-domain optical coherence tomography to measure macular and perimacular ganglion cell complex thicknesses and compare retinal ganglion cell parameters between algorithms. Methods. Ninety-two nonglaucomatous eyes from 92 participants underwent macular a...

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Main Authors: Dorothy S. K. Ng, Preeti Gupta, Yih Chung Tham, Chye Fong Peck, Tien Yin Wong, Mohammad Kamran Ikram, Carol Y. Cheung
Format: Article
Language:English
Published: Wiley 2015-01-01
Series:Journal of Ophthalmology
Online Access:http://dx.doi.org/10.1155/2015/605940
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author Dorothy S. K. Ng
Preeti Gupta
Yih Chung Tham
Chye Fong Peck
Tien Yin Wong
Mohammad Kamran Ikram
Carol Y. Cheung
author_facet Dorothy S. K. Ng
Preeti Gupta
Yih Chung Tham
Chye Fong Peck
Tien Yin Wong
Mohammad Kamran Ikram
Carol Y. Cheung
author_sort Dorothy S. K. Ng
collection DOAJ
description Purpose. To assess the repeatability of spectral-domain optical coherence tomography to measure macular and perimacular ganglion cell complex thicknesses and compare retinal ganglion cell parameters between algorithms. Methods. Ninety-two nonglaucomatous eyes from 92 participants underwent macular and perimacular ganglion cell complex thickness measurement using OCT-HS100 Glaucoma 3D algorithm and these measurements were repeated for 34 subjects. All subjects also had macular ganglion cell-inner plexiform layer thickness measured by Cirrus HD-OCT Ganglion Cell Analysis algorithm. Intraclass correlation coefficient and Pearson’s correlation analyses were performed. Results. Subfields of both macular and perimacular ganglion cell complex thicknesses had high intraclass correlation coefficient values between 0.979 (95% confidence interval [CI]: 0.958–0.989) and 0.981 (95% CI: 0.963, 0.991) and between 0.70 (95% CI: 0.481–0.838) and 0.987 (95% CI: 0.956–0.989), respectively. The overall average ganglion cell complex and macular average ganglion cell-inner plexiform layer thicknesses were strongly correlated (r=0.83, P<0.001).  Conclusions. The assessment of macular and perimacular retinal ganglion cell parameters by OCT-HS100 Glaucoma 3D algorithm is highly repeatable, and strongly correlates to retinal ganglion cell parameters assessed by Ganglion Cell Analysis algorithm. A comprehensive evaluation of retinal ganglion cells may be possible with OCT-HS100.
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spelling doaj-art-5c4e199ec52f494eac96a2af9ab7225f2025-08-20T03:34:13ZengWileyJournal of Ophthalmology2090-004X2090-00582015-01-01201510.1155/2015/605940605940Repeatability of Perimacular Ganglion Cell Complex Analysis with Spectral-Domain Optical Coherence TomographyDorothy S. K. Ng0Preeti Gupta1Yih Chung Tham2Chye Fong Peck3Tien Yin Wong4Mohammad Kamran Ikram5Carol Y. Cheung6Singapore Eye Research Institute, Singapore National Eye Centre, 169856, SingaporeSingapore Eye Research Institute, Singapore National Eye Centre, 169856, SingaporeSingapore Eye Research Institute, Singapore National Eye Centre, 169856, SingaporeSingapore Eye Research Institute, Singapore National Eye Centre, 169856, SingaporeSingapore Eye Research Institute, Singapore National Eye Centre, 169856, SingaporeSingapore Eye Research Institute, Singapore National Eye Centre, 169856, SingaporeSingapore Eye Research Institute, Singapore National Eye Centre, 169856, SingaporePurpose. To assess the repeatability of spectral-domain optical coherence tomography to measure macular and perimacular ganglion cell complex thicknesses and compare retinal ganglion cell parameters between algorithms. Methods. Ninety-two nonglaucomatous eyes from 92 participants underwent macular and perimacular ganglion cell complex thickness measurement using OCT-HS100 Glaucoma 3D algorithm and these measurements were repeated for 34 subjects. All subjects also had macular ganglion cell-inner plexiform layer thickness measured by Cirrus HD-OCT Ganglion Cell Analysis algorithm. Intraclass correlation coefficient and Pearson’s correlation analyses were performed. Results. Subfields of both macular and perimacular ganglion cell complex thicknesses had high intraclass correlation coefficient values between 0.979 (95% confidence interval [CI]: 0.958–0.989) and 0.981 (95% CI: 0.963, 0.991) and between 0.70 (95% CI: 0.481–0.838) and 0.987 (95% CI: 0.956–0.989), respectively. The overall average ganglion cell complex and macular average ganglion cell-inner plexiform layer thicknesses were strongly correlated (r=0.83, P<0.001).  Conclusions. The assessment of macular and perimacular retinal ganglion cell parameters by OCT-HS100 Glaucoma 3D algorithm is highly repeatable, and strongly correlates to retinal ganglion cell parameters assessed by Ganglion Cell Analysis algorithm. A comprehensive evaluation of retinal ganglion cells may be possible with OCT-HS100.http://dx.doi.org/10.1155/2015/605940
spellingShingle Dorothy S. K. Ng
Preeti Gupta
Yih Chung Tham
Chye Fong Peck
Tien Yin Wong
Mohammad Kamran Ikram
Carol Y. Cheung
Repeatability of Perimacular Ganglion Cell Complex Analysis with Spectral-Domain Optical Coherence Tomography
Journal of Ophthalmology
title Repeatability of Perimacular Ganglion Cell Complex Analysis with Spectral-Domain Optical Coherence Tomography
title_full Repeatability of Perimacular Ganglion Cell Complex Analysis with Spectral-Domain Optical Coherence Tomography
title_fullStr Repeatability of Perimacular Ganglion Cell Complex Analysis with Spectral-Domain Optical Coherence Tomography
title_full_unstemmed Repeatability of Perimacular Ganglion Cell Complex Analysis with Spectral-Domain Optical Coherence Tomography
title_short Repeatability of Perimacular Ganglion Cell Complex Analysis with Spectral-Domain Optical Coherence Tomography
title_sort repeatability of perimacular ganglion cell complex analysis with spectral domain optical coherence tomography
url http://dx.doi.org/10.1155/2015/605940
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