The methylation of TXNRD2 with chronic heart failure: the interaction effect between methylation regulation and clinical parameters-- a single center pilot study

Abstract Objective This study investigated the potential role of TXNRD2_FA44 promoter methylation in chronic heart failure (CHF) and its influence on disease mechanisms. Additionally, we explored the association between specific Cytosine-phosphate-Guanine (CpG) site methylation levels within the TXN...

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Main Authors: Rongrong Chen, Xufei Zhao, Bingqing Zhi, Rongqiang Zhang
Format: Article
Language:English
Published: BMC 2025-08-01
Series:BMC Cardiovascular Disorders
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Online Access:https://doi.org/10.1186/s12872-025-04951-x
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Summary:Abstract Objective This study investigated the potential role of TXNRD2_FA44 promoter methylation in chronic heart failure (CHF) and its influence on disease mechanisms. Additionally, we explored the association between specific Cytosine-phosphate-Guanine (CpG) site methylation levels within the TXNRD2_FA44 promoter and various biochemical markers in CHF patients. Methods The study included 20 individuals suffering from chronic heart failure and an equal number of healthy participants. Using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF–MS), CpG sites in the TXNRD2_FA44 promoter region were pinpointed and measured. The comparison of methylation levels was conducted between CHF patients and healthy individuals. Statistics was used to analyze the relationship between TXNRD2_FA44_CpG_27.28.29 and various key biochemical indicators. Results Patients with CHF exhibited significantly higher methylation levels at TXNRD2_FA44_CpG_27.28.29 compared to controls (P = 0.0008). This elevated methylation level correlated with levels of creatinine (Crea), estimated glomerular filtration rate (eGFR), percentage of lymphocytes (LYMPH), albumin (ALB), globulin (GLB), and the albumin-to-globin ratio (A/G) in CHF patients. Conclusions The methylation level of TXNRD2-FA44 in patients with chronic heart failure is significantly higher than controls, and the methylation level of TXNRD2-FA44 is related to multiple liver and kidney function indicators. These findings suggest that TXNRD2-FA44 could serve as a biomarker for CHF diagnosis and may affect the occurrence and development of CHF through liver and kidney function.
ISSN:1471-2261