Optimization of extraction in supercritical fluids in obtaining Pouteria lucuma seed oil by response surface methodology and artificial neuronal network coupled with a genetic algorithm
When processing lucuma (Pouteria lucuma), waste such as shells and seeds is generated, which is a source of bioactive compounds. Recently, lucuma seed (LS), especially its oily fraction, has been studied for containing phytosterols and tocopherols, powerful antioxidants with health benefits. This st...
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2024.1491479/full |
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| author | Alex Chauca-Cerrutti Marianela Inga José Luis Pasquel-Reátegui Indira Betalleluz-Pallardel Gustavo Puma-Isuiza |
| author_facet | Alex Chauca-Cerrutti Marianela Inga José Luis Pasquel-Reátegui Indira Betalleluz-Pallardel Gustavo Puma-Isuiza |
| author_sort | Alex Chauca-Cerrutti |
| collection | DOAJ |
| description | When processing lucuma (Pouteria lucuma), waste such as shells and seeds is generated, which is a source of bioactive compounds. Recently, lucuma seed (LS), especially its oily fraction, has been studied for containing phytosterols and tocopherols, powerful antioxidants with health benefits. This study proposes lucuma seed oil (LSO) extraction using supercritical fluid (SCF) to improve the quality of the extract and minimize the environmental impact. LS was previously characterized, and the extraction parameters were optimized using a Box-Behnken design, considering temperature (40–60°C), pressure (100–300 bar), and CO2 flow rate (3–7 mL/min), applying the response surface methodology (RSM) and neural networks with genetic algorithm (ANN+GA). The optimal parameters were 45°C, 300 bar, and 6 mL/min, obtaining 97.35% of the total oil content. The RSM and ANN+GA models showed R2 values of 0.9891 and 0.9999 respectively, indicating that both models exhibited a good fit to the experimental data. However, ANN+GA provided a greater proportion of the total variability, which facilitates the identification of the optimal parameters for the extraction of oil from lucuma seeds. Compared to the Soxhlet method, the LSO obtained by SCF presented better acidity (4.127 mg KOH/g), iodine (100.294 g I2/100 g), and refraction indices (1.4710), as well as to a higher content of mono- and polyunsaturated fatty acids. Supercritical CO2 extraction is presented as a sustainable green alternative to Soxhlet extraction for extracting oil from lucuma seed due to its high extraction efficiency and similar fatty acid profile. |
| format | Article |
| id | doaj-art-5c3091cd656a4376b985ef15f7bd5517 |
| institution | OA Journals |
| issn | 2296-2646 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj-art-5c3091cd656a4376b985ef15f7bd55172025-08-20T01:56:17ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462024-12-011210.3389/fchem.2024.14914791491479Optimization of extraction in supercritical fluids in obtaining Pouteria lucuma seed oil by response surface methodology and artificial neuronal network coupled with a genetic algorithmAlex Chauca-Cerrutti0Marianela Inga1José Luis Pasquel-Reátegui2Indira Betalleluz-Pallardel3Gustavo Puma-Isuiza4Facultad de Industrias Alimentarias, Universidad Nacional Agraria La Molina, Lima, PeruFacultad de Industrias Alimentarias, Universidad Nacional Agraria La Molina, Lima, PeruGrupo de Investigación en Ingeniería y Tecnología Agroindustrial, Facultad de Ingeniería Agroindustrial, Universidad Nacional de San Martín (UNSM), Tarapoto, San Martin, PeruFacultad de Industrias Alimentarias, Universidad Nacional Agraria La Molina, Lima, PeruFacultad de Industrias Alimentarias, Universidad Nacional Agraria La Molina, Lima, PeruWhen processing lucuma (Pouteria lucuma), waste such as shells and seeds is generated, which is a source of bioactive compounds. Recently, lucuma seed (LS), especially its oily fraction, has been studied for containing phytosterols and tocopherols, powerful antioxidants with health benefits. This study proposes lucuma seed oil (LSO) extraction using supercritical fluid (SCF) to improve the quality of the extract and minimize the environmental impact. LS was previously characterized, and the extraction parameters were optimized using a Box-Behnken design, considering temperature (40–60°C), pressure (100–300 bar), and CO2 flow rate (3–7 mL/min), applying the response surface methodology (RSM) and neural networks with genetic algorithm (ANN+GA). The optimal parameters were 45°C, 300 bar, and 6 mL/min, obtaining 97.35% of the total oil content. The RSM and ANN+GA models showed R2 values of 0.9891 and 0.9999 respectively, indicating that both models exhibited a good fit to the experimental data. However, ANN+GA provided a greater proportion of the total variability, which facilitates the identification of the optimal parameters for the extraction of oil from lucuma seeds. Compared to the Soxhlet method, the LSO obtained by SCF presented better acidity (4.127 mg KOH/g), iodine (100.294 g I2/100 g), and refraction indices (1.4710), as well as to a higher content of mono- and polyunsaturated fatty acids. Supercritical CO2 extraction is presented as a sustainable green alternative to Soxhlet extraction for extracting oil from lucuma seed due to its high extraction efficiency and similar fatty acid profile.https://www.frontiersin.org/articles/10.3389/fchem.2024.1491479/fullcircular economypolyunsaturated fatty acidspolyphenolsantioxidant activityagro-industrial wasteecofriendly |
| spellingShingle | Alex Chauca-Cerrutti Marianela Inga José Luis Pasquel-Reátegui Indira Betalleluz-Pallardel Gustavo Puma-Isuiza Optimization of extraction in supercritical fluids in obtaining Pouteria lucuma seed oil by response surface methodology and artificial neuronal network coupled with a genetic algorithm Frontiers in Chemistry circular economy polyunsaturated fatty acids polyphenols antioxidant activity agro-industrial waste ecofriendly |
| title | Optimization of extraction in supercritical fluids in obtaining Pouteria lucuma seed oil by response surface methodology and artificial neuronal network coupled with a genetic algorithm |
| title_full | Optimization of extraction in supercritical fluids in obtaining Pouteria lucuma seed oil by response surface methodology and artificial neuronal network coupled with a genetic algorithm |
| title_fullStr | Optimization of extraction in supercritical fluids in obtaining Pouteria lucuma seed oil by response surface methodology and artificial neuronal network coupled with a genetic algorithm |
| title_full_unstemmed | Optimization of extraction in supercritical fluids in obtaining Pouteria lucuma seed oil by response surface methodology and artificial neuronal network coupled with a genetic algorithm |
| title_short | Optimization of extraction in supercritical fluids in obtaining Pouteria lucuma seed oil by response surface methodology and artificial neuronal network coupled with a genetic algorithm |
| title_sort | optimization of extraction in supercritical fluids in obtaining pouteria lucuma seed oil by response surface methodology and artificial neuronal network coupled with a genetic algorithm |
| topic | circular economy polyunsaturated fatty acids polyphenols antioxidant activity agro-industrial waste ecofriendly |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2024.1491479/full |
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