A Phenylalanine Ammonia Lyase Capacitive Sensor for Phenylalanine Detection
In this paper, an easy-to-use and fast biosensor for phenylalanine quantification in patients affected by phenylketonuria is investigated. The phenylalanine concentration was indirectly estimated through the ammonia released as a by-product of an enzymatic reaction, which was then detected by exploi...
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MDPI AG
2024-03-01
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| author | Bruno Andò Salvatore Castorina Ludovica Maugeri Salvatore Petralia Maria Anna Messina Martino Ruggieri Giovanni Neri Angelo Ferlazzo Emilio Sardini Mauro Serpelloni |
| author_facet | Bruno Andò Salvatore Castorina Ludovica Maugeri Salvatore Petralia Maria Anna Messina Martino Ruggieri Giovanni Neri Angelo Ferlazzo Emilio Sardini Mauro Serpelloni |
| author_sort | Bruno Andò |
| collection | DOAJ |
| description | In this paper, an easy-to-use and fast biosensor for phenylalanine quantification in patients affected by phenylketonuria is investigated. The phenylalanine concentration was indirectly estimated through the ammonia released as a by-product of an enzymatic reaction, which was then detected by exploiting an yttria-stabilized zirconia layer deposited over an interdigitated capacitive sensor. The latter was manufactured by rapid-prototyping technologies. A sensor limit of detection higher than 1.25 µM was estimated, along with an accuracy better than 18.31 µM. |
| format | Article |
| id | doaj-art-5c19d288d2bf46c2b785673a22901ee4 |
| institution | DOAJ |
| issn | 2504-3900 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Proceedings |
| spelling | doaj-art-5c19d288d2bf46c2b785673a22901ee42025-08-20T02:57:00ZengMDPI AGProceedings2504-39002024-03-019715110.3390/proceedings2024097051A Phenylalanine Ammonia Lyase Capacitive Sensor for Phenylalanine DetectionBruno Andò0Salvatore Castorina1Ludovica Maugeri2Salvatore Petralia3Maria Anna Messina4Martino Ruggieri5Giovanni Neri6Angelo Ferlazzo7Emilio Sardini8Mauro Serpelloni9DIEEI, University of Catania, 95123 Catania, ItalyDIEEI, University of Catania, 95123 Catania, ItalyDIEEI, University of Catania, 95123 Catania, ItalyDepartment of Drug and Health Sciences, University of Catania, 95123 Catania, ItalyExpanded Newborn Screening Laboratory, Unit of Clinical Pediatrics, University of Catania, 95123 Catania, ItalyUnit of Clinical Pediatrics, Department of Clinical and Experimental Medicine, University of Catania, 95124 Catania, ItalyDepartment of Engineering, University of Messina, 98122 Messina, ItalyDepartment of Engineering, University of Messina, 98122 Messina, ItalyDepartment of Information Engineering, University of Brescia, 25123 Brescia, ItalyDepartment of Information Engineering, University of Brescia, 25123 Brescia, ItalyIn this paper, an easy-to-use and fast biosensor for phenylalanine quantification in patients affected by phenylketonuria is investigated. The phenylalanine concentration was indirectly estimated through the ammonia released as a by-product of an enzymatic reaction, which was then detected by exploiting an yttria-stabilized zirconia layer deposited over an interdigitated capacitive sensor. The latter was manufactured by rapid-prototyping technologies. A sensor limit of detection higher than 1.25 µM was estimated, along with an accuracy better than 18.31 µM.https://www.mdpi.com/2504-3900/97/1/51phenylalanineammoniabiosensorinterdigitated capacitorrapid prototyping |
| spellingShingle | Bruno Andò Salvatore Castorina Ludovica Maugeri Salvatore Petralia Maria Anna Messina Martino Ruggieri Giovanni Neri Angelo Ferlazzo Emilio Sardini Mauro Serpelloni A Phenylalanine Ammonia Lyase Capacitive Sensor for Phenylalanine Detection Proceedings phenylalanine ammonia biosensor interdigitated capacitor rapid prototyping |
| title | A Phenylalanine Ammonia Lyase Capacitive Sensor for Phenylalanine Detection |
| title_full | A Phenylalanine Ammonia Lyase Capacitive Sensor for Phenylalanine Detection |
| title_fullStr | A Phenylalanine Ammonia Lyase Capacitive Sensor for Phenylalanine Detection |
| title_full_unstemmed | A Phenylalanine Ammonia Lyase Capacitive Sensor for Phenylalanine Detection |
| title_short | A Phenylalanine Ammonia Lyase Capacitive Sensor for Phenylalanine Detection |
| title_sort | phenylalanine ammonia lyase capacitive sensor for phenylalanine detection |
| topic | phenylalanine ammonia biosensor interdigitated capacitor rapid prototyping |
| url | https://www.mdpi.com/2504-3900/97/1/51 |
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