Improving the Proteome-Mining of <i>Schizophyllum commune</i> to Enhance Medicinal Mushroom Applications
This study presents the first comprehensive proteomic profile of an Italian strain of <i>Schizophyllum commune</i>, a highly heterogeneous white-rot fungal species with significant potential for industrial, nutraceutical, cosmeceutical, and clinical applications. Three protein extraction...
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| Language: | English |
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MDPI AG
2025-02-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/11/2/120 |
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| author | Anthea Desiderio Lorenzo Goppa Carlo Santambrogio Stefania Brocca Simone Buratti Carolina Elena Girometta Meghma Sarkar Maria Teresa Venuti Elena Savino Paola Rossi Emanuele Ferrari |
| author_facet | Anthea Desiderio Lorenzo Goppa Carlo Santambrogio Stefania Brocca Simone Buratti Carolina Elena Girometta Meghma Sarkar Maria Teresa Venuti Elena Savino Paola Rossi Emanuele Ferrari |
| author_sort | Anthea Desiderio |
| collection | DOAJ |
| description | This study presents the first comprehensive proteomic profile of an Italian strain of <i>Schizophyllum commune</i>, a highly heterogeneous white-rot fungal species with significant potential for industrial, nutraceutical, cosmeceutical, and clinical applications. Three protein extraction methods and their impact on yield and resulting protein composition have been compared. Results revealed that the combination of Tris–Cl and urea increases the total protein yield and the variety of enzymatic species related to pivotal pathways. Notably, over 2000 proteins were identified, including enzymes involved in the growth and development of mycelium, trehalose biosynthesis, and different types of carbohydrate-active enzymes (CAZymes). These enzymes are crucial for nutraceutical and agro-industrial applications of <i>S. commune</i>. The multiple-step proteomic approach used could be a model for investigating other fungal species. |
| format | Article |
| id | doaj-art-5c1802d9b39e48bdace8fbfd90bc6ae2 |
| institution | DOAJ |
| issn | 2309-608X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj-art-5c1802d9b39e48bdace8fbfd90bc6ae22025-08-20T02:45:00ZengMDPI AGJournal of Fungi2309-608X2025-02-0111212010.3390/jof11020120Improving the Proteome-Mining of <i>Schizophyllum commune</i> to Enhance Medicinal Mushroom ApplicationsAnthea Desiderio0Lorenzo Goppa1Carlo Santambrogio2Stefania Brocca3Simone Buratti4Carolina Elena Girometta5Meghma Sarkar6Maria Teresa Venuti7Elena Savino8Paola Rossi9Emanuele Ferrari10Department of Earth and Environmental Sciences (DSTA), University of Pavia, 27100 Pavia, ItalyDepartment of Earth and Environmental Sciences (DSTA), University of Pavia, 27100 Pavia, ItalyDepartment of Biotechnology and Biosciences, University of Milano-Bicocca, 20126 Milan, ItalyDepartment of Biotechnology and Biosciences, University of Milano-Bicocca, 20126 Milan, ItalyDepartment of Earth and Environmental Sciences (DSTA), University of Pavia, 27100 Pavia, ItalyDepartment of Earth and Environmental Sciences (DSTA), University of Pavia, 27100 Pavia, ItalyDepartment of Biology and Biotechnology “L. Spallanzani”, University of Pavia, 27100 Pavia, ItalyDepartment of Biology and Biotechnology “L. Spallanzani”, University of Pavia, 27100 Pavia, ItalyDepartment of Earth and Environmental Sciences (DSTA), University of Pavia, 27100 Pavia, ItalyDepartment of Biology and Biotechnology “L. Spallanzani”, University of Pavia, 27100 Pavia, ItalyMolecular Ecology Group (MEG), Water Research Institute (CNR-IRSA), National Research Council of Italy, 28922 Verbania, ItalyThis study presents the first comprehensive proteomic profile of an Italian strain of <i>Schizophyllum commune</i>, a highly heterogeneous white-rot fungal species with significant potential for industrial, nutraceutical, cosmeceutical, and clinical applications. Three protein extraction methods and their impact on yield and resulting protein composition have been compared. Results revealed that the combination of Tris–Cl and urea increases the total protein yield and the variety of enzymatic species related to pivotal pathways. Notably, over 2000 proteins were identified, including enzymes involved in the growth and development of mycelium, trehalose biosynthesis, and different types of carbohydrate-active enzymes (CAZymes). These enzymes are crucial for nutraceutical and agro-industrial applications of <i>S. commune</i>. The multiple-step proteomic approach used could be a model for investigating other fungal species.https://www.mdpi.com/2309-608X/11/2/120protein isolationproteomicsextraction methodmedicinal mushroommycelium |
| spellingShingle | Anthea Desiderio Lorenzo Goppa Carlo Santambrogio Stefania Brocca Simone Buratti Carolina Elena Girometta Meghma Sarkar Maria Teresa Venuti Elena Savino Paola Rossi Emanuele Ferrari Improving the Proteome-Mining of <i>Schizophyllum commune</i> to Enhance Medicinal Mushroom Applications Journal of Fungi protein isolation proteomics extraction method medicinal mushroom mycelium |
| title | Improving the Proteome-Mining of <i>Schizophyllum commune</i> to Enhance Medicinal Mushroom Applications |
| title_full | Improving the Proteome-Mining of <i>Schizophyllum commune</i> to Enhance Medicinal Mushroom Applications |
| title_fullStr | Improving the Proteome-Mining of <i>Schizophyllum commune</i> to Enhance Medicinal Mushroom Applications |
| title_full_unstemmed | Improving the Proteome-Mining of <i>Schizophyllum commune</i> to Enhance Medicinal Mushroom Applications |
| title_short | Improving the Proteome-Mining of <i>Schizophyllum commune</i> to Enhance Medicinal Mushroom Applications |
| title_sort | improving the proteome mining of i schizophyllum commune i to enhance medicinal mushroom applications |
| topic | protein isolation proteomics extraction method medicinal mushroom mycelium |
| url | https://www.mdpi.com/2309-608X/11/2/120 |
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