Rutin Protects Pancreatic Islets Against Methylglyoxal-Induced Oxidative Stress and Elevates Insulin Secretion
Objective: Hyperglycemia is the main characteristic of diabetes, which leads to complications, including oxidative stress (OS). Pancreatic β-cells are susceptible to damage against OS, which results in a disruption in insulin secretion. The current study focused on the antioxidant features of rutin...
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Galenos Publishing House
2025-04-01
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| Series: | Gazi Medical Journal |
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| Online Access: | https://gazimedj.com/articles/rutin-protects-pancreatic-islets-against-methylglyoxal-induced-oxidative-stress-and-elevates-insulin-secretion/doi/gmj.2024.4236 |
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| author | Elnaz Harooni Vahid Radmehr Reza Noei Razliqi Akram Ahangarpour Hadi Bazyar |
| author_facet | Elnaz Harooni Vahid Radmehr Reza Noei Razliqi Akram Ahangarpour Hadi Bazyar |
| author_sort | Elnaz Harooni |
| collection | DOAJ |
| description | Objective: Hyperglycemia is the main characteristic of diabetes, which leads to complications, including oxidative stress (OS). Pancreatic β-cells are susceptible to damage against OS, which results in a disruption in insulin secretion. The current study focused on the antioxidant features of rutin (Rtn) flavonoid to prevent methylglyoxal (MG-)induced oxidative damage in pancreatic islets isolated from mice.
Methods: After isolating islets from twenty-four male mice, we conducted two experimental parts, (1) with exposure to MG- and (2) without exposure to MG. Experiments were carried out in both culture media with 5.6- and 16.7-mM glucose concentrations. Islets were transferred to the culture medium and exposed to different substances. In the end, insulin secretion, antioxidant enzyme activities, and malondialdehyde (MDA) concentration were investigated by ELISA.
Results: The reduced levels of insulin in MG-exposed islets (p=0.01, p<0.001 in 5.6- and 16.7-mM glucose concentration, respectively) were reversed by Rtn treatment. MG- increased MDA levels in 5.6- and 16.7-mM glucose concentrations (p<0.001 for 5.6 mM and p=0.005 for 16.7 mM. Also, we observed a remarkable decrease in the activities of catalase, superoxide dismutase, and glutathione peroxidase due to 300 μM MG- exposure in islets, in 5.6- and 16.7-mM glucose concentrations. Rtn at doses of 1 and 2 µM significantly reduced MDA levels. Moreover, Rtn had beneficial effects on antioxidant activities.
Conclusion: Rtn significantly protected pancreatic islets by reducing lipid peroxidation and enhancing antioxidant activity. The decreased insulin levels in MG-exposed islets were effectively restored in the Rtn-treated groups. |
| format | Article |
| id | doaj-art-5c151515b2214402bd01ac3dacd68f49 |
| institution | DOAJ |
| issn | 2147-2092 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Gazi Medical Journal |
| spelling | doaj-art-5c151515b2214402bd01ac3dacd68f492025-08-20T03:10:22ZengGalenos Publishing HouseGazi Medical Journal2147-20922025-04-0136217718310.12996/gmj.2024.4236Rutin Protects Pancreatic Islets Against Methylglyoxal-Induced Oxidative Stress and Elevates Insulin SecretionElnaz Harooni0https://orcid.org/0000-0002-0775-7933Vahid Radmehr1https://orcid.org/0000-0002-1889-2233Reza Noei Razliqi2https://orcid.org/0000-0002-4249-0322Akram Ahangarpour3https://orcid.org/0000-0002-9534-9699Hadi Bazyar4https://orcid.org/0000-0002-1627-7122Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranDiabetes Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranDepartment of Physiology, Faculty of Medicine, Islamic Azad University Tehran Medical Sciences, Tehran, IranPersian Gulf Physiology Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranDepartment of Public Health, Sirjan School of Medical Sciences, Sirjan, IranObjective: Hyperglycemia is the main characteristic of diabetes, which leads to complications, including oxidative stress (OS). Pancreatic β-cells are susceptible to damage against OS, which results in a disruption in insulin secretion. The current study focused on the antioxidant features of rutin (Rtn) flavonoid to prevent methylglyoxal (MG-)induced oxidative damage in pancreatic islets isolated from mice. Methods: After isolating islets from twenty-four male mice, we conducted two experimental parts, (1) with exposure to MG- and (2) without exposure to MG. Experiments were carried out in both culture media with 5.6- and 16.7-mM glucose concentrations. Islets were transferred to the culture medium and exposed to different substances. In the end, insulin secretion, antioxidant enzyme activities, and malondialdehyde (MDA) concentration were investigated by ELISA. Results: The reduced levels of insulin in MG-exposed islets (p=0.01, p<0.001 in 5.6- and 16.7-mM glucose concentration, respectively) were reversed by Rtn treatment. MG- increased MDA levels in 5.6- and 16.7-mM glucose concentrations (p<0.001 for 5.6 mM and p=0.005 for 16.7 mM. Also, we observed a remarkable decrease in the activities of catalase, superoxide dismutase, and glutathione peroxidase due to 300 μM MG- exposure in islets, in 5.6- and 16.7-mM glucose concentrations. Rtn at doses of 1 and 2 µM significantly reduced MDA levels. Moreover, Rtn had beneficial effects on antioxidant activities. Conclusion: Rtn significantly protected pancreatic islets by reducing lipid peroxidation and enhancing antioxidant activity. The decreased insulin levels in MG-exposed islets were effectively restored in the Rtn-treated groups.https://gazimedj.com/articles/rutin-protects-pancreatic-islets-against-methylglyoxal-induced-oxidative-stress-and-elevates-insulin-secretion/doi/gmj.2024.4236flavonoidshyperglycemiainsulinmethylglyoxaloxidative stressrutin |
| spellingShingle | Elnaz Harooni Vahid Radmehr Reza Noei Razliqi Akram Ahangarpour Hadi Bazyar Rutin Protects Pancreatic Islets Against Methylglyoxal-Induced Oxidative Stress and Elevates Insulin Secretion Gazi Medical Journal flavonoids hyperglycemia insulin methylglyoxal oxidative stress rutin |
| title | Rutin Protects Pancreatic Islets Against Methylglyoxal-Induced Oxidative Stress and Elevates Insulin Secretion |
| title_full | Rutin Protects Pancreatic Islets Against Methylglyoxal-Induced Oxidative Stress and Elevates Insulin Secretion |
| title_fullStr | Rutin Protects Pancreatic Islets Against Methylglyoxal-Induced Oxidative Stress and Elevates Insulin Secretion |
| title_full_unstemmed | Rutin Protects Pancreatic Islets Against Methylglyoxal-Induced Oxidative Stress and Elevates Insulin Secretion |
| title_short | Rutin Protects Pancreatic Islets Against Methylglyoxal-Induced Oxidative Stress and Elevates Insulin Secretion |
| title_sort | rutin protects pancreatic islets against methylglyoxal induced oxidative stress and elevates insulin secretion |
| topic | flavonoids hyperglycemia insulin methylglyoxal oxidative stress rutin |
| url | https://gazimedj.com/articles/rutin-protects-pancreatic-islets-against-methylglyoxal-induced-oxidative-stress-and-elevates-insulin-secretion/doi/gmj.2024.4236 |
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