Single-cell transcriptome atlas of lamprey exploring Natterin- induced white adipose tissue browning
Abstract Lampreys are early jawless vertebrates that are the key to understanding the evolution of vertebrates. However, the lack of cytomic studies on multiple lamprey organs has hindered progress in this field. Therefore, the present study constructed a comprehensive cell atlas comprising 604,460...
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| Format: | Article |
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Nature Portfolio
2025-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-56153-w |
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| author | Yue Pang Yating Qin Zeyu Du Qun Liu Jin Zhang Kai Han Jiali Lu Zengbao Yuan Jun Li Shanshan Pan Xinrui Dong Mengyang Xu Dantong Wang Shuo Li Zhen Li Yadong Chen Zhisheng Zhao Zhe Zhang Shunqin Chuan Yue Song Mingjie Sun Xiaodong Jia Zhangyong Xia Liping Zhan Zhen Yue Wei Cui Jun Wang Ying Gu Ming Ni Huanming Yang Xun Xu Xin Liu Qingwei Li Guangyi Fan |
| author_facet | Yue Pang Yating Qin Zeyu Du Qun Liu Jin Zhang Kai Han Jiali Lu Zengbao Yuan Jun Li Shanshan Pan Xinrui Dong Mengyang Xu Dantong Wang Shuo Li Zhen Li Yadong Chen Zhisheng Zhao Zhe Zhang Shunqin Chuan Yue Song Mingjie Sun Xiaodong Jia Zhangyong Xia Liping Zhan Zhen Yue Wei Cui Jun Wang Ying Gu Ming Ni Huanming Yang Xun Xu Xin Liu Qingwei Li Guangyi Fan |
| author_sort | Yue Pang |
| collection | DOAJ |
| description | Abstract Lampreys are early jawless vertebrates that are the key to understanding the evolution of vertebrates. However, the lack of cytomic studies on multiple lamprey organs has hindered progress in this field. Therefore, the present study constructed a comprehensive cell atlas comprising 604,460 cells/nuclei and 70 cell types from 14 lamprey tissue samples. Comparison of cellular evolution across species revealed that most lamprey cell types are homologous to those in jawed vertebrates. We discovered acinar- and islet-like cell populations despite the lack of parenchymal organs in lampreys, providing evidence of pancreatic function in vertebrates. Furthermore, we investigated the heterogeneity of lamprey immune cell populations. Natterin was highly expressed in granulocytes, and NATTERIN was localized to the lipid droplets. Moreover, we developed a transgenic mouse model expressing Natterin to elucidate the role of NATTERIN in lipid metabolism, whereas the browning of white adipose tissue was induced. These findings elucidate vertebrate cellular evolution and advance our understanding of adipose tissue plasticity and metabolic regulation in lampreys. |
| format | Article |
| id | doaj-art-5c0e380745cf4c279bbcdf66732dcf49 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-5c0e380745cf4c279bbcdf66732dcf492025-02-09T12:43:54ZengNature PortfolioNature Communications2041-17232025-01-0116111410.1038/s41467-025-56153-wSingle-cell transcriptome atlas of lamprey exploring Natterin- induced white adipose tissue browningYue Pang0Yating Qin1Zeyu Du2Qun Liu3Jin Zhang4Kai Han5Jiali Lu6Zengbao Yuan7Jun Li8Shanshan Pan9Xinrui Dong10Mengyang Xu11Dantong Wang12Shuo Li13Zhen Li14Yadong Chen15Zhisheng Zhao16Zhe Zhang17Shunqin Chuan18Yue Song19Mingjie Sun20Xiaodong Jia21Zhangyong Xia22Liping Zhan23Zhen Yue24Wei Cui25Jun Wang26Ying Gu27Ming Ni28Huanming Yang29Xun Xu30Xin Liu31Qingwei Li32Guangyi Fan33College of Life Science, Liaoning Normal UniversityBGI ResearchCollege of Life Science, Liaoning Normal UniversityBGI ResearchCollege of Life Science, Liaoning Normal UniversityBGI ResearchCollege of Life Science, Liaoning Normal UniversityBGI ResearchCollege of Life Science, Liaoning Normal UniversityBGI ResearchCollege of Life Science, Liaoning Normal UniversityBGI ResearchBGI ResearchState Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery SciencesBGI ResearchBGI ResearchCollege of Life Science, Liaoning Normal UniversityMGI TechCollege of Life Science, Liaoning Normal UniversityBGI ResearchCollege of Life Science, Liaoning Normal UniversityJoint Laboratory for Translational Medicine Research, Liaocheng People’s HospitalDepartment of Neurology, The Second People’s Hospital of LiaochengBGI ResearchBGI ResearchBGI ResearchBGI ResearchBGI ResearchMGI TechBGI ResearchBGI ResearchBGI ResearchCollege of Life Science, Liaoning Normal UniversityBGI ResearchAbstract Lampreys are early jawless vertebrates that are the key to understanding the evolution of vertebrates. However, the lack of cytomic studies on multiple lamprey organs has hindered progress in this field. Therefore, the present study constructed a comprehensive cell atlas comprising 604,460 cells/nuclei and 70 cell types from 14 lamprey tissue samples. Comparison of cellular evolution across species revealed that most lamprey cell types are homologous to those in jawed vertebrates. We discovered acinar- and islet-like cell populations despite the lack of parenchymal organs in lampreys, providing evidence of pancreatic function in vertebrates. Furthermore, we investigated the heterogeneity of lamprey immune cell populations. Natterin was highly expressed in granulocytes, and NATTERIN was localized to the lipid droplets. Moreover, we developed a transgenic mouse model expressing Natterin to elucidate the role of NATTERIN in lipid metabolism, whereas the browning of white adipose tissue was induced. These findings elucidate vertebrate cellular evolution and advance our understanding of adipose tissue plasticity and metabolic regulation in lampreys.https://doi.org/10.1038/s41467-025-56153-w |
| spellingShingle | Yue Pang Yating Qin Zeyu Du Qun Liu Jin Zhang Kai Han Jiali Lu Zengbao Yuan Jun Li Shanshan Pan Xinrui Dong Mengyang Xu Dantong Wang Shuo Li Zhen Li Yadong Chen Zhisheng Zhao Zhe Zhang Shunqin Chuan Yue Song Mingjie Sun Xiaodong Jia Zhangyong Xia Liping Zhan Zhen Yue Wei Cui Jun Wang Ying Gu Ming Ni Huanming Yang Xun Xu Xin Liu Qingwei Li Guangyi Fan Single-cell transcriptome atlas of lamprey exploring Natterin- induced white adipose tissue browning Nature Communications |
| title | Single-cell transcriptome atlas of lamprey exploring Natterin- induced white adipose tissue browning |
| title_full | Single-cell transcriptome atlas of lamprey exploring Natterin- induced white adipose tissue browning |
| title_fullStr | Single-cell transcriptome atlas of lamprey exploring Natterin- induced white adipose tissue browning |
| title_full_unstemmed | Single-cell transcriptome atlas of lamprey exploring Natterin- induced white adipose tissue browning |
| title_short | Single-cell transcriptome atlas of lamprey exploring Natterin- induced white adipose tissue browning |
| title_sort | single cell transcriptome atlas of lamprey exploring natterin induced white adipose tissue browning |
| url | https://doi.org/10.1038/s41467-025-56153-w |
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