A previously unidentified circRNA inhibits virus replication by regulating the miR-24-3p/KEAP1 axis.
Circular RNAs (circRNAs) exert diverse biological functions in different processes. However, the role of circRNAs during virus infection is mostly unknown. Herein, we explored the characteristics of host circRNAs using alphaherpesvirus pseudorabies virus (PRV) as a model. PRV infection upregulated t...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2024-12-01
|
| Series: | PLoS Pathogens |
| Online Access: | https://doi.org/10.1371/journal.ppat.1012712 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850028722850103296 |
|---|---|
| author | Haimin Li Liuyang Du Juan Li Yanming Huang Chenhe Lu Tingjuan Deng Yan Yan Yulan Jin Wei Wu Jinyan Gu Jiyong Zhou |
| author_facet | Haimin Li Liuyang Du Juan Li Yanming Huang Chenhe Lu Tingjuan Deng Yan Yan Yulan Jin Wei Wu Jinyan Gu Jiyong Zhou |
| author_sort | Haimin Li |
| collection | DOAJ |
| description | Circular RNAs (circRNAs) exert diverse biological functions in different processes. However, the role of circRNAs during virus infection is mostly unknown. Herein, we explored the characteristics of host circRNAs using alphaherpesvirus pseudorabies virus (PRV) as a model. PRV infection upregulated the expression of circRNA circ29164, which does not encode a protein. RNA pulldown assays identified that circ29164 interacts with the microRNA ssc-miRNA-24-3p. Further analysis indicated that ssc-miR-24-3p targets the mRNA encoding kelch-like ECH-associated protein 1 (KEAP1), and circ29164 competitively binds to ssc-miR-24-3p to prevent it binding to Keap1. Apoptosis detection demonstrated that circ29164 or Keap1 overexpression, but not knockdown, induced caspase 3 activity and the release of cytochrome C from mitochondria, and inhibited PRV replication. Taken together, these data identified a previously undiscovered circRNA, circ29164, which inhibits PRV replication by competitively binding to ssc-24-3p to maintain KEAP1 levels. |
| format | Article |
| id | doaj-art-5c0ceee2f6b94556a4292c52d535995d |
| institution | DOAJ |
| issn | 1553-7366 1553-7374 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj-art-5c0ceee2f6b94556a4292c52d535995d2025-08-20T02:59:45ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742024-12-012012e101271210.1371/journal.ppat.1012712A previously unidentified circRNA inhibits virus replication by regulating the miR-24-3p/KEAP1 axis.Haimin LiLiuyang DuJuan LiYanming HuangChenhe LuTingjuan DengYan YanYulan JinWei WuJinyan GuJiyong ZhouCircular RNAs (circRNAs) exert diverse biological functions in different processes. However, the role of circRNAs during virus infection is mostly unknown. Herein, we explored the characteristics of host circRNAs using alphaherpesvirus pseudorabies virus (PRV) as a model. PRV infection upregulated the expression of circRNA circ29164, which does not encode a protein. RNA pulldown assays identified that circ29164 interacts with the microRNA ssc-miRNA-24-3p. Further analysis indicated that ssc-miR-24-3p targets the mRNA encoding kelch-like ECH-associated protein 1 (KEAP1), and circ29164 competitively binds to ssc-miR-24-3p to prevent it binding to Keap1. Apoptosis detection demonstrated that circ29164 or Keap1 overexpression, but not knockdown, induced caspase 3 activity and the release of cytochrome C from mitochondria, and inhibited PRV replication. Taken together, these data identified a previously undiscovered circRNA, circ29164, which inhibits PRV replication by competitively binding to ssc-24-3p to maintain KEAP1 levels.https://doi.org/10.1371/journal.ppat.1012712 |
| spellingShingle | Haimin Li Liuyang Du Juan Li Yanming Huang Chenhe Lu Tingjuan Deng Yan Yan Yulan Jin Wei Wu Jinyan Gu Jiyong Zhou A previously unidentified circRNA inhibits virus replication by regulating the miR-24-3p/KEAP1 axis. PLoS Pathogens |
| title | A previously unidentified circRNA inhibits virus replication by regulating the miR-24-3p/KEAP1 axis. |
| title_full | A previously unidentified circRNA inhibits virus replication by regulating the miR-24-3p/KEAP1 axis. |
| title_fullStr | A previously unidentified circRNA inhibits virus replication by regulating the miR-24-3p/KEAP1 axis. |
| title_full_unstemmed | A previously unidentified circRNA inhibits virus replication by regulating the miR-24-3p/KEAP1 axis. |
| title_short | A previously unidentified circRNA inhibits virus replication by regulating the miR-24-3p/KEAP1 axis. |
| title_sort | previously unidentified circrna inhibits virus replication by regulating the mir 24 3p keap1 axis |
| url | https://doi.org/10.1371/journal.ppat.1012712 |
| work_keys_str_mv | AT haiminli apreviouslyunidentifiedcircrnainhibitsvirusreplicationbyregulatingthemir243pkeap1axis AT liuyangdu apreviouslyunidentifiedcircrnainhibitsvirusreplicationbyregulatingthemir243pkeap1axis AT juanli apreviouslyunidentifiedcircrnainhibitsvirusreplicationbyregulatingthemir243pkeap1axis AT yanminghuang apreviouslyunidentifiedcircrnainhibitsvirusreplicationbyregulatingthemir243pkeap1axis AT chenhelu apreviouslyunidentifiedcircrnainhibitsvirusreplicationbyregulatingthemir243pkeap1axis AT tingjuandeng apreviouslyunidentifiedcircrnainhibitsvirusreplicationbyregulatingthemir243pkeap1axis AT yanyan apreviouslyunidentifiedcircrnainhibitsvirusreplicationbyregulatingthemir243pkeap1axis AT yulanjin apreviouslyunidentifiedcircrnainhibitsvirusreplicationbyregulatingthemir243pkeap1axis AT weiwu apreviouslyunidentifiedcircrnainhibitsvirusreplicationbyregulatingthemir243pkeap1axis AT jinyangu apreviouslyunidentifiedcircrnainhibitsvirusreplicationbyregulatingthemir243pkeap1axis AT jiyongzhou apreviouslyunidentifiedcircrnainhibitsvirusreplicationbyregulatingthemir243pkeap1axis AT haiminli previouslyunidentifiedcircrnainhibitsvirusreplicationbyregulatingthemir243pkeap1axis AT liuyangdu previouslyunidentifiedcircrnainhibitsvirusreplicationbyregulatingthemir243pkeap1axis AT juanli previouslyunidentifiedcircrnainhibitsvirusreplicationbyregulatingthemir243pkeap1axis AT yanminghuang previouslyunidentifiedcircrnainhibitsvirusreplicationbyregulatingthemir243pkeap1axis AT chenhelu previouslyunidentifiedcircrnainhibitsvirusreplicationbyregulatingthemir243pkeap1axis AT tingjuandeng previouslyunidentifiedcircrnainhibitsvirusreplicationbyregulatingthemir243pkeap1axis AT yanyan previouslyunidentifiedcircrnainhibitsvirusreplicationbyregulatingthemir243pkeap1axis AT yulanjin previouslyunidentifiedcircrnainhibitsvirusreplicationbyregulatingthemir243pkeap1axis AT weiwu previouslyunidentifiedcircrnainhibitsvirusreplicationbyregulatingthemir243pkeap1axis AT jinyangu previouslyunidentifiedcircrnainhibitsvirusreplicationbyregulatingthemir243pkeap1axis AT jiyongzhou previouslyunidentifiedcircrnainhibitsvirusreplicationbyregulatingthemir243pkeap1axis |