Protective Effects of Astaxanthin against Oxidative Stress: Attenuation of TNF-α-Induced Oxidative Damage in SW480 Cells and Azoxymethane/Dextran Sulfate Sodium-Induced Colitis-Associated Cancer in C57BL/6 Mice
In this study, we investigated the protective effects of astaxanthin (AST) against oxidative stress induced by the combination of azoxymethane (AOM) and dextran sulfate sodium (DSS) in colitis-associated cancer (CAC) and TNF-α-induced human colorectal cancer cells (SW480), as well as the underlying...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-10-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1660-3397/22/10/469 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850206097918394368 |
|---|---|
| author | Haifeng Zhang Min Wang Yu Zhou Shaojie Bao Feng Wang Chunmei Li |
| author_facet | Haifeng Zhang Min Wang Yu Zhou Shaojie Bao Feng Wang Chunmei Li |
| author_sort | Haifeng Zhang |
| collection | DOAJ |
| description | In this study, we investigated the protective effects of astaxanthin (AST) against oxidative stress induced by the combination of azoxymethane (AOM) and dextran sulfate sodium (DSS) in colitis-associated cancer (CAC) and TNF-α-induced human colorectal cancer cells (SW480), as well as the underlying mechanism. In vitro experiments revealed that astaxanthin reduced reactive oxygen species (ROS) generation and inhibited the expression of Phosphorylated JNK (P-JNK), Phosphorylated ERK (P-ERK), Phosphorylated p65 (P-p65), and the NF-κB downstream protein cyclooxygenase-2 (COX-2). In vivo experiments showed that astaxanthin ameliorated AOM/DSS-induced weight loss, shortened the colon length, and caused histomorphological changes. In addition, astaxanthin suppressed cellular inflammation by modulating the MAPK and NF-κB pathways and inhibiting the expression of the proinflammatory cytokines IL-6, IL-1β, and TNF-α. In conclusion, astaxanthin attenuates cellular inflammation and CAC through its antioxidant effects. |
| format | Article |
| id | doaj-art-5c03bdb93e384f32970e9a269a726310 |
| institution | OA Journals |
| issn | 1660-3397 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj-art-5c03bdb93e384f32970e9a269a7263102025-08-20T02:10:56ZengMDPI AGMarine Drugs1660-33972024-10-01221046910.3390/md22100469Protective Effects of Astaxanthin against Oxidative Stress: Attenuation of TNF-α-Induced Oxidative Damage in SW480 Cells and Azoxymethane/Dextran Sulfate Sodium-Induced Colitis-Associated Cancer in C57BL/6 MiceHaifeng Zhang0Min Wang1Yu Zhou2Shaojie Bao3Feng Wang4Chunmei Li5College of Tourism and Cuisine, Yangzhou University, Yangzhou 225127, ChinaCollege of Tourism and Cuisine, Yangzhou University, Yangzhou 225127, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaCollege of Tourism and Cuisine, Yangzhou University, Yangzhou 225127, ChinaCollege of Tourism and Cuisine, Yangzhou University, Yangzhou 225127, ChinaIn this study, we investigated the protective effects of astaxanthin (AST) against oxidative stress induced by the combination of azoxymethane (AOM) and dextran sulfate sodium (DSS) in colitis-associated cancer (CAC) and TNF-α-induced human colorectal cancer cells (SW480), as well as the underlying mechanism. In vitro experiments revealed that astaxanthin reduced reactive oxygen species (ROS) generation and inhibited the expression of Phosphorylated JNK (P-JNK), Phosphorylated ERK (P-ERK), Phosphorylated p65 (P-p65), and the NF-κB downstream protein cyclooxygenase-2 (COX-2). In vivo experiments showed that astaxanthin ameliorated AOM/DSS-induced weight loss, shortened the colon length, and caused histomorphological changes. In addition, astaxanthin suppressed cellular inflammation by modulating the MAPK and NF-κB pathways and inhibiting the expression of the proinflammatory cytokines IL-6, IL-1β, and TNF-α. In conclusion, astaxanthin attenuates cellular inflammation and CAC through its antioxidant effects.https://www.mdpi.com/1660-3397/22/10/469astaxanthinoxidative stressinflammationcolitis-associated cancer |
| spellingShingle | Haifeng Zhang Min Wang Yu Zhou Shaojie Bao Feng Wang Chunmei Li Protective Effects of Astaxanthin against Oxidative Stress: Attenuation of TNF-α-Induced Oxidative Damage in SW480 Cells and Azoxymethane/Dextran Sulfate Sodium-Induced Colitis-Associated Cancer in C57BL/6 Mice Marine Drugs astaxanthin oxidative stress inflammation colitis-associated cancer |
| title | Protective Effects of Astaxanthin against Oxidative Stress: Attenuation of TNF-α-Induced Oxidative Damage in SW480 Cells and Azoxymethane/Dextran Sulfate Sodium-Induced Colitis-Associated Cancer in C57BL/6 Mice |
| title_full | Protective Effects of Astaxanthin against Oxidative Stress: Attenuation of TNF-α-Induced Oxidative Damage in SW480 Cells and Azoxymethane/Dextran Sulfate Sodium-Induced Colitis-Associated Cancer in C57BL/6 Mice |
| title_fullStr | Protective Effects of Astaxanthin against Oxidative Stress: Attenuation of TNF-α-Induced Oxidative Damage in SW480 Cells and Azoxymethane/Dextran Sulfate Sodium-Induced Colitis-Associated Cancer in C57BL/6 Mice |
| title_full_unstemmed | Protective Effects of Astaxanthin against Oxidative Stress: Attenuation of TNF-α-Induced Oxidative Damage in SW480 Cells and Azoxymethane/Dextran Sulfate Sodium-Induced Colitis-Associated Cancer in C57BL/6 Mice |
| title_short | Protective Effects of Astaxanthin against Oxidative Stress: Attenuation of TNF-α-Induced Oxidative Damage in SW480 Cells and Azoxymethane/Dextran Sulfate Sodium-Induced Colitis-Associated Cancer in C57BL/6 Mice |
| title_sort | protective effects of astaxanthin against oxidative stress attenuation of tnf α induced oxidative damage in sw480 cells and azoxymethane dextran sulfate sodium induced colitis associated cancer in c57bl 6 mice |
| topic | astaxanthin oxidative stress inflammation colitis-associated cancer |
| url | https://www.mdpi.com/1660-3397/22/10/469 |
| work_keys_str_mv | AT haifengzhang protectiveeffectsofastaxanthinagainstoxidativestressattenuationoftnfainducedoxidativedamageinsw480cellsandazoxymethanedextransulfatesodiuminducedcolitisassociatedcancerinc57bl6mice AT minwang protectiveeffectsofastaxanthinagainstoxidativestressattenuationoftnfainducedoxidativedamageinsw480cellsandazoxymethanedextransulfatesodiuminducedcolitisassociatedcancerinc57bl6mice AT yuzhou protectiveeffectsofastaxanthinagainstoxidativestressattenuationoftnfainducedoxidativedamageinsw480cellsandazoxymethanedextransulfatesodiuminducedcolitisassociatedcancerinc57bl6mice AT shaojiebao protectiveeffectsofastaxanthinagainstoxidativestressattenuationoftnfainducedoxidativedamageinsw480cellsandazoxymethanedextransulfatesodiuminducedcolitisassociatedcancerinc57bl6mice AT fengwang protectiveeffectsofastaxanthinagainstoxidativestressattenuationoftnfainducedoxidativedamageinsw480cellsandazoxymethanedextransulfatesodiuminducedcolitisassociatedcancerinc57bl6mice AT chunmeili protectiveeffectsofastaxanthinagainstoxidativestressattenuationoftnfainducedoxidativedamageinsw480cellsandazoxymethanedextransulfatesodiuminducedcolitisassociatedcancerinc57bl6mice |