Glycyrol targets Pneumolysin (PLY) oligomerization to reduce Streptococcus pneumoniae toxicity
Aim of the studyExploring the potential of glycyrol to reduce the invasiveness of Streptococcus pneumoniae (S. pneumoniae).Materials and MethodsCell experiments were performed using A549 alveolar epithelial cells and S. pneumoniae D39. Glycyrol was added to A549 cells mixed with or without Pneumolys...
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2024.1478135/full |
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| author | Yudi Li Yudi Li Hongji Wu Hongji Wu Yibo Hu Yibo Hu Haoji Meng Haoji Meng Yan Xu Yan Xu |
| author_facet | Yudi Li Yudi Li Hongji Wu Hongji Wu Yibo Hu Yibo Hu Haoji Meng Haoji Meng Yan Xu Yan Xu |
| author_sort | Yudi Li |
| collection | DOAJ |
| description | Aim of the studyExploring the potential of glycyrol to reduce the invasiveness of Streptococcus pneumoniae (S. pneumoniae).Materials and MethodsCell experiments were performed using A549 alveolar epithelial cells and S. pneumoniae D39. Glycyrol was added to A549 cells mixed with or without Pneumolysin (PLY) to detect the effect of Glycyrol on PLY toxicity. Glycyrol was used to detect the effect on S. pneumoniae toxicity and PLY production. Mice was used to detect the anti-infectious ability of Glycyrol to regulate S. pneumoniae infection. Western blot and Molecular docking were used to detect how and where Glycyrol inhibits PLY toxicity.ResultsWe discovered that glycyrol, a main component of the widely recognized Chinese herbal medicine licorice, reduce the virulence of PLY in S. pneumoniae invasion; glycyrol achieves this effect by interacting with PLY through hydrogen bonding, van der Waals interactions, and solvation effects to reduce the pore-forming toxicity of PLY. Moreover, glycyrol did not affect the growth of S. pneumoniae or the production of PLY.ConclusionWe have actually discovered that Glycyrol, a major component of the widely known Chinese herbal medicine Glycyrrhiza uralensis Fisch., interacts with PLY through hydrogen bonds, Van der Waals and solvation to reduce the pore-forming toxicity of PLY and the toxicity of S. pneumoniae invasion, while not affecting the growth of S. pneumoniae and the production of PLY. |
| format | Article |
| id | doaj-art-5c01a7a0f5994202bf6f1e687dd32c1e |
| institution | OA Journals |
| issn | 1663-9812 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Pharmacology |
| spelling | doaj-art-5c01a7a0f5994202bf6f1e687dd32c1e2025-08-20T02:38:51ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122024-12-011510.3389/fphar.2024.14781351478135Glycyrol targets Pneumolysin (PLY) oligomerization to reduce Streptococcus pneumoniae toxicityYudi Li0Yudi Li1Hongji Wu2Hongji Wu3Yibo Hu4Yibo Hu5Haoji Meng6Haoji Meng7Yan Xu8Yan Xu9Department of Pediatrics, The First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou, Henan, ChinaSchool of Pediatrics, Henan University of Chinese Medicine, Zhengzhou, Henan, ChinaDepartment of Pediatrics, The First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou, Henan, ChinaSchool of Pediatrics, Henan University of Chinese Medicine, Zhengzhou, Henan, ChinaDepartment of Pediatrics, The First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou, Henan, ChinaSchool of Pediatrics, Henan University of Chinese Medicine, Zhengzhou, Henan, ChinaDepartment of Pediatrics, The First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou, Henan, ChinaSchool of Pediatrics, Henan University of Chinese Medicine, Zhengzhou, Henan, ChinaDepartment of Pediatrics, The First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou, Henan, ChinaSchool of Pediatrics, Henan University of Chinese Medicine, Zhengzhou, Henan, ChinaAim of the studyExploring the potential of glycyrol to reduce the invasiveness of Streptococcus pneumoniae (S. pneumoniae).Materials and MethodsCell experiments were performed using A549 alveolar epithelial cells and S. pneumoniae D39. Glycyrol was added to A549 cells mixed with or without Pneumolysin (PLY) to detect the effect of Glycyrol on PLY toxicity. Glycyrol was used to detect the effect on S. pneumoniae toxicity and PLY production. Mice was used to detect the anti-infectious ability of Glycyrol to regulate S. pneumoniae infection. Western blot and Molecular docking were used to detect how and where Glycyrol inhibits PLY toxicity.ResultsWe discovered that glycyrol, a main component of the widely recognized Chinese herbal medicine licorice, reduce the virulence of PLY in S. pneumoniae invasion; glycyrol achieves this effect by interacting with PLY through hydrogen bonding, van der Waals interactions, and solvation effects to reduce the pore-forming toxicity of PLY. Moreover, glycyrol did not affect the growth of S. pneumoniae or the production of PLY.ConclusionWe have actually discovered that Glycyrol, a major component of the widely known Chinese herbal medicine Glycyrrhiza uralensis Fisch., interacts with PLY through hydrogen bonds, Van der Waals and solvation to reduce the pore-forming toxicity of PLY and the toxicity of S. pneumoniae invasion, while not affecting the growth of S. pneumoniae and the production of PLY.https://www.frontiersin.org/articles/10.3389/fphar.2024.1478135/fullglycyrolPLYStreptococcus pneumoniaeTCMtoxicity |
| spellingShingle | Yudi Li Yudi Li Hongji Wu Hongji Wu Yibo Hu Yibo Hu Haoji Meng Haoji Meng Yan Xu Yan Xu Glycyrol targets Pneumolysin (PLY) oligomerization to reduce Streptococcus pneumoniae toxicity Frontiers in Pharmacology glycyrol PLY Streptococcus pneumoniae TCM toxicity |
| title | Glycyrol targets Pneumolysin (PLY) oligomerization to reduce Streptococcus pneumoniae toxicity |
| title_full | Glycyrol targets Pneumolysin (PLY) oligomerization to reduce Streptococcus pneumoniae toxicity |
| title_fullStr | Glycyrol targets Pneumolysin (PLY) oligomerization to reduce Streptococcus pneumoniae toxicity |
| title_full_unstemmed | Glycyrol targets Pneumolysin (PLY) oligomerization to reduce Streptococcus pneumoniae toxicity |
| title_short | Glycyrol targets Pneumolysin (PLY) oligomerization to reduce Streptococcus pneumoniae toxicity |
| title_sort | glycyrol targets pneumolysin ply oligomerization to reduce streptococcus pneumoniae toxicity |
| topic | glycyrol PLY Streptococcus pneumoniae TCM toxicity |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2024.1478135/full |
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