The role of CD83 in the pathogenesis of immune thrombocytopenia
Background CD83 are closely related to the pathogenesis of immune thrombocytopenia (ITP), but the exact mechanism remains unclear.Aim To explore the relationship between CD83 and CD4+ T cell subsets and clarify the role of CD83 in the pathogenesis of ITP.Methods RT-qPCR and Flow cytometry were used...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Hematology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/16078454.2024.2372482 |
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| author | Xiuli Wang Qiyuan Zhou Wen Yang Hui Bi Honghui Wang Yacan Wang Yadong Du Lin Liu Yuebo Liu Liefen Yin Jin Yao Jingxing Yu Wei Tao Yongchun Zhou Zeping Zhou |
| author_facet | Xiuli Wang Qiyuan Zhou Wen Yang Hui Bi Honghui Wang Yacan Wang Yadong Du Lin Liu Yuebo Liu Liefen Yin Jin Yao Jingxing Yu Wei Tao Yongchun Zhou Zeping Zhou |
| author_sort | Xiuli Wang |
| collection | DOAJ |
| description | Background CD83 are closely related to the pathogenesis of immune thrombocytopenia (ITP), but the exact mechanism remains unclear.Aim To explore the relationship between CD83 and CD4+ T cell subsets and clarify the role of CD83 in the pathogenesis of ITP.Methods RT-qPCR and Flow cytometry were used to illustrate CD83 expression. The downregulation and overexpression of DC-CD83 were co-cultured with CD4+ T cells to detect cell proliferation, co-cultured supernatant cytokines and Tregs expression.Results The results indicate that the ITP patients showed higher expression of CD83 than the healthy controls. The proliferation of CD4+ T cells was inhibited by downregulation of DCs-CD83 but promoted by overexpression of DCs-CD83. siRNA-CD83 inhibited proinflammatory IFN-γ and IL-17 secretion while raising TGF-β, IL-10 concentrations. Overexpression of DCs-CD83 promoted Tregs expression.Conclusion The Th1/Th2 and Th17/Tregs polarization were reversed via interfering DCs with siRNA-CD83. CD83 plays an important role in ITP pathogenesis, suggesting novel treatment for ITP patients. |
| format | Article |
| id | doaj-art-5bfe3cf7066540f4befc463c2904532a |
| institution | OA Journals |
| issn | 1607-8454 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Hematology |
| spelling | doaj-art-5bfe3cf7066540f4befc463c2904532a2025-08-20T01:59:09ZengTaylor & Francis GroupHematology1607-84542024-12-0129110.1080/16078454.2024.2372482The role of CD83 in the pathogenesis of immune thrombocytopeniaXiuli Wang0Qiyuan Zhou1Wen Yang2Hui Bi3Honghui Wang4Yacan Wang5Yadong Du6Lin Liu7Yuebo Liu8Liefen Yin9Jin Yao10Jingxing Yu11Wei Tao12Yongchun Zhou13Zeping Zhou14Department of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, People’s Republic of ChinaDepartment of Molecular and Cellular Biology, University of California, Davis, USADepartment of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, People’s Republic of ChinaDepartment of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, People’s Republic of ChinaDepartment of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, People’s Republic of ChinaDepartment of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, People’s Republic of ChinaDepartment of Hematology, The First Affiliated Hospital of Henan University of Science and Technology, Henan, People's Republic of ChinaDepartment of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, People’s Republic of ChinaDepartment of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, People’s Republic of ChinaDepartment of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, People’s Republic of ChinaDepartment of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, People’s Republic of ChinaDepartment of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, People’s Republic of ChinaDepartment of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, People’s Republic of ChinaDepartment of Molecular Diagnostic Center, Kunming Medical University, School of Clinical Oncology: Yunnan Cancer Hospital, Kunming, People’s Republic of ChinaDepartment of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, People’s Republic of ChinaBackground CD83 are closely related to the pathogenesis of immune thrombocytopenia (ITP), but the exact mechanism remains unclear.Aim To explore the relationship between CD83 and CD4+ T cell subsets and clarify the role of CD83 in the pathogenesis of ITP.Methods RT-qPCR and Flow cytometry were used to illustrate CD83 expression. The downregulation and overexpression of DC-CD83 were co-cultured with CD4+ T cells to detect cell proliferation, co-cultured supernatant cytokines and Tregs expression.Results The results indicate that the ITP patients showed higher expression of CD83 than the healthy controls. The proliferation of CD4+ T cells was inhibited by downregulation of DCs-CD83 but promoted by overexpression of DCs-CD83. siRNA-CD83 inhibited proinflammatory IFN-γ and IL-17 secretion while raising TGF-β, IL-10 concentrations. Overexpression of DCs-CD83 promoted Tregs expression.Conclusion The Th1/Th2 and Th17/Tregs polarization were reversed via interfering DCs with siRNA-CD83. CD83 plays an important role in ITP pathogenesis, suggesting novel treatment for ITP patients.https://www.tandfonline.com/doi/10.1080/16078454.2024.2372482Immune thrombocytopeniaCD83siRNAcytokinesdendritic cellsimmune |
| spellingShingle | Xiuli Wang Qiyuan Zhou Wen Yang Hui Bi Honghui Wang Yacan Wang Yadong Du Lin Liu Yuebo Liu Liefen Yin Jin Yao Jingxing Yu Wei Tao Yongchun Zhou Zeping Zhou The role of CD83 in the pathogenesis of immune thrombocytopenia Hematology Immune thrombocytopenia CD83 siRNA cytokines dendritic cells immune |
| title | The role of CD83 in the pathogenesis of immune thrombocytopenia |
| title_full | The role of CD83 in the pathogenesis of immune thrombocytopenia |
| title_fullStr | The role of CD83 in the pathogenesis of immune thrombocytopenia |
| title_full_unstemmed | The role of CD83 in the pathogenesis of immune thrombocytopenia |
| title_short | The role of CD83 in the pathogenesis of immune thrombocytopenia |
| title_sort | role of cd83 in the pathogenesis of immune thrombocytopenia |
| topic | Immune thrombocytopenia CD83 siRNA cytokines dendritic cells immune |
| url | https://www.tandfonline.com/doi/10.1080/16078454.2024.2372482 |
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