Development of Murine Hepatic NK Cells during Ontogeny: Comparison with Spleen NK Cells

The phenotype of developing liver NK cells (CD3−NK1.1+) was investigated during mouse ontogeny comparing with spleen NK cells. The highest percentage of hepatic CD27−CD11b− NK cells occurred at the fetal stage. After birth, the percentage of CD27−CD11b−NK cells in both the liver and spleen gradually...

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Main Authors: Xian Wu, Yongyan Chen, Haiming Wei, Rui Sun, Zhigang Tian
Format: Article
Language:English
Published: Wiley 2012-01-01
Series:Clinical and Developmental Immunology
Online Access:http://dx.doi.org/10.1155/2012/759765
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author Xian Wu
Yongyan Chen
Haiming Wei
Rui Sun
Zhigang Tian
author_facet Xian Wu
Yongyan Chen
Haiming Wei
Rui Sun
Zhigang Tian
author_sort Xian Wu
collection DOAJ
description The phenotype of developing liver NK cells (CD3−NK1.1+) was investigated during mouse ontogeny comparing with spleen NK cells. The highest percentage of hepatic CD27−CD11b− NK cells occurred at the fetal stage. After birth, the percentage of CD27−CD11b−NK cells in both the liver and spleen gradually decreased to their lowest level at 6 weeks. More CD27+CD11b−NK cells were detected in the liver than that in spleen from week 1 to 6. Expression of NKG2A on liver NK cells was decreased but still much higher than that of spleen NK cells after 1 week. The NKG2D expression on liver NK cells increased to its highest level and was significantly higher than on spleen NK cells till 4 weeks. During mouse ontogeny, weaker expression of NKp46 and CD2 and stronger expression of CD69, CD11c, 2B4, and CD73 were observed on liver NK cells. Furthermore, neonatal liver NK cells express higher IFN-γ and perforin than adult .These results suggest that the maturation process of NK cells is unique in the livers, and liver microenvironments might play critical roles to keep NK cells in an immature status.
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series Clinical and Developmental Immunology
spelling doaj-art-5bfdf7e5cce84739a072f1b4cbe60afc2025-02-03T01:23:47ZengWileyClinical and Developmental Immunology1740-25221740-25302012-01-01201210.1155/2012/759765759765Development of Murine Hepatic NK Cells during Ontogeny: Comparison with Spleen NK CellsXian Wu0Yongyan Chen1Haiming Wei2Rui Sun3Zhigang Tian4Institute of Immunology, Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei 230027, ChinaInstitute of Immunology, Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei 230027, ChinaInstitute of Immunology, Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei 230027, ChinaInstitute of Immunology, Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei 230027, ChinaInstitute of Immunology, Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei 230027, ChinaThe phenotype of developing liver NK cells (CD3−NK1.1+) was investigated during mouse ontogeny comparing with spleen NK cells. The highest percentage of hepatic CD27−CD11b− NK cells occurred at the fetal stage. After birth, the percentage of CD27−CD11b−NK cells in both the liver and spleen gradually decreased to their lowest level at 6 weeks. More CD27+CD11b−NK cells were detected in the liver than that in spleen from week 1 to 6. Expression of NKG2A on liver NK cells was decreased but still much higher than that of spleen NK cells after 1 week. The NKG2D expression on liver NK cells increased to its highest level and was significantly higher than on spleen NK cells till 4 weeks. During mouse ontogeny, weaker expression of NKp46 and CD2 and stronger expression of CD69, CD11c, 2B4, and CD73 were observed on liver NK cells. Furthermore, neonatal liver NK cells express higher IFN-γ and perforin than adult .These results suggest that the maturation process of NK cells is unique in the livers, and liver microenvironments might play critical roles to keep NK cells in an immature status.http://dx.doi.org/10.1155/2012/759765
spellingShingle Xian Wu
Yongyan Chen
Haiming Wei
Rui Sun
Zhigang Tian
Development of Murine Hepatic NK Cells during Ontogeny: Comparison with Spleen NK Cells
Clinical and Developmental Immunology
title Development of Murine Hepatic NK Cells during Ontogeny: Comparison with Spleen NK Cells
title_full Development of Murine Hepatic NK Cells during Ontogeny: Comparison with Spleen NK Cells
title_fullStr Development of Murine Hepatic NK Cells during Ontogeny: Comparison with Spleen NK Cells
title_full_unstemmed Development of Murine Hepatic NK Cells during Ontogeny: Comparison with Spleen NK Cells
title_short Development of Murine Hepatic NK Cells during Ontogeny: Comparison with Spleen NK Cells
title_sort development of murine hepatic nk cells during ontogeny comparison with spleen nk cells
url http://dx.doi.org/10.1155/2012/759765
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AT yongyanchen developmentofmurinehepaticnkcellsduringontogenycomparisonwithspleennkcells
AT haimingwei developmentofmurinehepaticnkcellsduringontogenycomparisonwithspleennkcells
AT ruisun developmentofmurinehepaticnkcellsduringontogenycomparisonwithspleennkcells
AT zhigangtian developmentofmurinehepaticnkcellsduringontogenycomparisonwithspleennkcells