Development of Murine Hepatic NK Cells during Ontogeny: Comparison with Spleen NK Cells
The phenotype of developing liver NK cells (CD3−NK1.1+) was investigated during mouse ontogeny comparing with spleen NK cells. The highest percentage of hepatic CD27−CD11b− NK cells occurred at the fetal stage. After birth, the percentage of CD27−CD11b−NK cells in both the liver and spleen gradually...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2012/759765 |
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| author | Xian Wu Yongyan Chen Haiming Wei Rui Sun Zhigang Tian |
| author_facet | Xian Wu Yongyan Chen Haiming Wei Rui Sun Zhigang Tian |
| author_sort | Xian Wu |
| collection | DOAJ |
| description | The phenotype of developing liver NK cells (CD3−NK1.1+) was investigated during mouse ontogeny comparing with spleen NK cells. The highest percentage of hepatic CD27−CD11b− NK cells occurred at the fetal stage. After birth, the percentage of CD27−CD11b−NK cells in both the liver and spleen gradually decreased to their lowest level at 6 weeks. More CD27+CD11b−NK cells were detected in the liver than that in spleen from week 1 to 6. Expression of NKG2A on liver NK cells was decreased but still much higher than that of spleen NK cells after 1 week. The NKG2D expression on liver NK cells increased to its highest level and was significantly higher than on spleen NK cells till 4 weeks. During mouse ontogeny, weaker expression of NKp46 and CD2 and stronger expression of CD69, CD11c, 2B4, and CD73 were observed on liver NK cells. Furthermore, neonatal liver NK cells express higher IFN-γ and perforin than adult .These results suggest that the maturation process of NK cells is unique in the livers, and liver microenvironments might play critical roles to keep NK cells in an immature status. |
| format | Article |
| id | doaj-art-5bfdf7e5cce84739a072f1b4cbe60afc |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-5bfdf7e5cce84739a072f1b4cbe60afc2025-02-03T01:23:47ZengWileyClinical and Developmental Immunology1740-25221740-25302012-01-01201210.1155/2012/759765759765Development of Murine Hepatic NK Cells during Ontogeny: Comparison with Spleen NK CellsXian Wu0Yongyan Chen1Haiming Wei2Rui Sun3Zhigang Tian4Institute of Immunology, Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei 230027, ChinaInstitute of Immunology, Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei 230027, ChinaInstitute of Immunology, Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei 230027, ChinaInstitute of Immunology, Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei 230027, ChinaInstitute of Immunology, Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei 230027, ChinaThe phenotype of developing liver NK cells (CD3−NK1.1+) was investigated during mouse ontogeny comparing with spleen NK cells. The highest percentage of hepatic CD27−CD11b− NK cells occurred at the fetal stage. After birth, the percentage of CD27−CD11b−NK cells in both the liver and spleen gradually decreased to their lowest level at 6 weeks. More CD27+CD11b−NK cells were detected in the liver than that in spleen from week 1 to 6. Expression of NKG2A on liver NK cells was decreased but still much higher than that of spleen NK cells after 1 week. The NKG2D expression on liver NK cells increased to its highest level and was significantly higher than on spleen NK cells till 4 weeks. During mouse ontogeny, weaker expression of NKp46 and CD2 and stronger expression of CD69, CD11c, 2B4, and CD73 were observed on liver NK cells. Furthermore, neonatal liver NK cells express higher IFN-γ and perforin than adult .These results suggest that the maturation process of NK cells is unique in the livers, and liver microenvironments might play critical roles to keep NK cells in an immature status.http://dx.doi.org/10.1155/2012/759765 |
| spellingShingle | Xian Wu Yongyan Chen Haiming Wei Rui Sun Zhigang Tian Development of Murine Hepatic NK Cells during Ontogeny: Comparison with Spleen NK Cells Clinical and Developmental Immunology |
| title | Development of Murine Hepatic NK Cells during Ontogeny: Comparison with Spleen NK Cells |
| title_full | Development of Murine Hepatic NK Cells during Ontogeny: Comparison with Spleen NK Cells |
| title_fullStr | Development of Murine Hepatic NK Cells during Ontogeny: Comparison with Spleen NK Cells |
| title_full_unstemmed | Development of Murine Hepatic NK Cells during Ontogeny: Comparison with Spleen NK Cells |
| title_short | Development of Murine Hepatic NK Cells during Ontogeny: Comparison with Spleen NK Cells |
| title_sort | development of murine hepatic nk cells during ontogeny comparison with spleen nk cells |
| url | http://dx.doi.org/10.1155/2012/759765 |
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