EXPERIMENTAL STRATEGY FOR IN VITRO EVALUATION OF CYTOTOXIC / ANTITUMOR PROPERTIES OF NEW METAL COMPLEXES WITH DIFFERENT LIGANDS
PURPOSE. The aim of this study was to evaluate cytotoxic / antitumor properties of newly synthesized metal [Zn(II), Cu(II), Co(II), Ni(II), Zn(II)/Ag(I), Zn(II)/Au(I)] complexes with various ligands (Schiff bases, non-steroidal anti-inflammatory drugs, bile acids) and to introduce an optimized strat...
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| Language: | English |
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Trakia University
2019-11-01
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| Series: | Trakia Journal of Sciences |
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| Online Access: | http://tru.uni-sz.bg/tsj/Volume%2017,%202019,%20Supplement%202,%20Series%20Biomedical%20Sciences/19_R.Alexandrova.pdf |
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| author | R. Alexandrova R. Spasov Z. Petrova T. Zhivkova L. Dyakova D. Dinev M. Glavcheva B. Andonova-Lilova A. Abudalleh M. Georgieva G. Miloshev D.-C. Culita G. Marinescu V. Alexeeva C. Podlipnik |
| author_facet | R. Alexandrova R. Spasov Z. Petrova T. Zhivkova L. Dyakova D. Dinev M. Glavcheva B. Andonova-Lilova A. Abudalleh M. Georgieva G. Miloshev D.-C. Culita G. Marinescu V. Alexeeva C. Podlipnik |
| author_sort | R. Alexandrova |
| collection | DOAJ |
| description | PURPOSE. The aim of this study was to evaluate cytotoxic / antitumor properties of newly synthesized metal [Zn(II), Cu(II), Co(II), Ni(II), Zn(II)/Ag(I), Zn(II)/Au(I)] complexes with various ligands (Schiff bases, non-steroidal anti-inflammatory drugs, bile acids) and to introduce an optimized strategy for
such investigations in our research activity.
MATERIALS AND METHODS. Human and animal tumor and non-tumor cells were used as model systems. Short-term (3 – 96h) and long-term (> 2 weeks) experiments were carried out using cytotoxicity assays, cytological / immunocytochemical, biochemical and molecular-biological methods
to assess the influence of the compounds on cell viability and proliferation and their ability to induce apoptosis/necrosis and/or autophagy.
RESULTS. The examined metal complexes express cytotoxic activity that is time- and concentrationdependent and are more active than the corresponding ligands tested alone. Zn(II)/Au(I) and Zn(II)/Ag(I) complexes with Salen are found to be the most promising cytotoxic agents being effective
also in multidrug resistant cancer cells. Their cytotoxic activity is higher than those of cisplatin, oxaliplatin and epirubicin.
CONCLUSIONS. A complex approach, based on a wide range of cell cultures and methods with different molecular / cellular targets and mechanisms of action was optimized and successfully applied for the assessment of cytotoxicity of new metal complexes. |
| format | Article |
| id | doaj-art-5bf0b7f3d1074cd59e4f2a897bad3604 |
| institution | DOAJ |
| issn | 1313-3551 |
| language | English |
| publishDate | 2019-11-01 |
| publisher | Trakia University |
| record_format | Article |
| series | Trakia Journal of Sciences |
| spelling | doaj-art-5bf0b7f3d1074cd59e4f2a897bad36042025-08-20T02:55:03ZengTrakia UniversityTrakia Journal of Sciences1313-35512019-11-0117Suppl. 2878910.15547/tjs.2019.s.02.019EXPERIMENTAL STRATEGY FOR IN VITRO EVALUATION OF CYTOTOXIC / ANTITUMOR PROPERTIES OF NEW METAL COMPLEXES WITH DIFFERENT LIGANDSR. AlexandrovaR. SpasovZ. PetrovaT. ZhivkovaL. DyakovaD. DinevM. GlavchevaB. Andonova-LilovaA. AbudallehM. GeorgievaG. MiloshevD.-C. CulitaG. MarinescuV. AlexeevaC. PodlipnikPURPOSE. The aim of this study was to evaluate cytotoxic / antitumor properties of newly synthesized metal [Zn(II), Cu(II), Co(II), Ni(II), Zn(II)/Ag(I), Zn(II)/Au(I)] complexes with various ligands (Schiff bases, non-steroidal anti-inflammatory drugs, bile acids) and to introduce an optimized strategy for such investigations in our research activity. MATERIALS AND METHODS. Human and animal tumor and non-tumor cells were used as model systems. Short-term (3 – 96h) and long-term (> 2 weeks) experiments were carried out using cytotoxicity assays, cytological / immunocytochemical, biochemical and molecular-biological methods to assess the influence of the compounds on cell viability and proliferation and their ability to induce apoptosis/necrosis and/or autophagy. RESULTS. The examined metal complexes express cytotoxic activity that is time- and concentrationdependent and are more active than the corresponding ligands tested alone. Zn(II)/Au(I) and Zn(II)/Ag(I) complexes with Salen are found to be the most promising cytotoxic agents being effective also in multidrug resistant cancer cells. Their cytotoxic activity is higher than those of cisplatin, oxaliplatin and epirubicin. CONCLUSIONS. A complex approach, based on a wide range of cell cultures and methods with different molecular / cellular targets and mechanisms of action was optimized and successfully applied for the assessment of cytotoxicity of new metal complexes.http://tru.uni-sz.bg/tsj/Volume%2017,%202019,%20Supplement%202,%20Series%20Biomedical%20Sciences/19_R.Alexandrova.pdfcell culturesmetal compoundscancercytotoxic / anticancer activityantitumor agentsapoptosisautophagy |
| spellingShingle | R. Alexandrova R. Spasov Z. Petrova T. Zhivkova L. Dyakova D. Dinev M. Glavcheva B. Andonova-Lilova A. Abudalleh M. Georgieva G. Miloshev D.-C. Culita G. Marinescu V. Alexeeva C. Podlipnik EXPERIMENTAL STRATEGY FOR IN VITRO EVALUATION OF CYTOTOXIC / ANTITUMOR PROPERTIES OF NEW METAL COMPLEXES WITH DIFFERENT LIGANDS Trakia Journal of Sciences cell cultures metal compounds cancer cytotoxic / anticancer activity antitumor agents apoptosis autophagy |
| title | EXPERIMENTAL STRATEGY FOR IN VITRO EVALUATION OF CYTOTOXIC / ANTITUMOR PROPERTIES OF NEW METAL COMPLEXES WITH DIFFERENT LIGANDS |
| title_full | EXPERIMENTAL STRATEGY FOR IN VITRO EVALUATION OF CYTOTOXIC / ANTITUMOR PROPERTIES OF NEW METAL COMPLEXES WITH DIFFERENT LIGANDS |
| title_fullStr | EXPERIMENTAL STRATEGY FOR IN VITRO EVALUATION OF CYTOTOXIC / ANTITUMOR PROPERTIES OF NEW METAL COMPLEXES WITH DIFFERENT LIGANDS |
| title_full_unstemmed | EXPERIMENTAL STRATEGY FOR IN VITRO EVALUATION OF CYTOTOXIC / ANTITUMOR PROPERTIES OF NEW METAL COMPLEXES WITH DIFFERENT LIGANDS |
| title_short | EXPERIMENTAL STRATEGY FOR IN VITRO EVALUATION OF CYTOTOXIC / ANTITUMOR PROPERTIES OF NEW METAL COMPLEXES WITH DIFFERENT LIGANDS |
| title_sort | experimental strategy for in vitro evaluation of cytotoxic antitumor properties of new metal complexes with different ligands |
| topic | cell cultures metal compounds cancer cytotoxic / anticancer activity antitumor agents apoptosis autophagy |
| url | http://tru.uni-sz.bg/tsj/Volume%2017,%202019,%20Supplement%202,%20Series%20Biomedical%20Sciences/19_R.Alexandrova.pdf |
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