The association between traumatic temporomandibular joint bony ankylosis and depressive disorder in growing rats
Abstract Background The psychological symptoms of temporomandibular joint (TMJ) ankylosis were similar to that of depressive disorder, but there were no relevant evidences to confirm that the humans or animals with TMJ ankylosis had depressive disorder. The aim of this study was to investigate the a...
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2025-04-01
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| Series: | BMC Oral Health |
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| Online Access: | https://doi.org/10.1186/s12903-025-05886-7 |
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| author | Tiange Deng Yang Xue Yiming Wang Xiang Guo Zhen Ma Hongzhi Zhou Zaiyong Yang Zhengwu Peng Xiangxiang Hu Ning Li Kaijin Hu Lei Wang |
| author_facet | Tiange Deng Yang Xue Yiming Wang Xiang Guo Zhen Ma Hongzhi Zhou Zaiyong Yang Zhengwu Peng Xiangxiang Hu Ning Li Kaijin Hu Lei Wang |
| author_sort | Tiange Deng |
| collection | DOAJ |
| description | Abstract Background The psychological symptoms of temporomandibular joint (TMJ) ankylosis were similar to that of depressive disorder, but there were no relevant evidences to confirm that the humans or animals with TMJ ankylosis had depressive disorder. The aim of this study was to investigate the association between TMJ ankylosis and depressive disorder in the rat model. Methods Thirty 3-week-old male Sprague–Dawley (SD) rats were used in this study. The damage of TMJ complexes and narrowed joint space were performed in the unilateral TMJ of test group to induce TMJ bony ankylosis (experimental side). The other TMJ of test group underwent a sham operation (sham side). The TMJs of control group did not undergo any operations. At 8 weeks postoperatively, behavioral tests, body weight, passive maximum mouth opening (PMMO), and TMJ morphological features were evaluated, and the hippocampuses were analyzed using western blotting and immunocytochemistry. The data was compared between the test group and control group by independent t-test, between the experimental side and sham side by paired t-test. The correlations between PMMO/area of bony fusion and duration of immobility, sucrose preference, CB1 receptor protein, mean optical density of CB1 receptor protein, and the number of BrdU-positive cell were evaluated using linear regression analysis. The level of significance was 0.05. Results In the test group, the traumatic TMJ complexes with narrowed joint space developed TMJ bony ankylosis, the area of bony mass of experimental side (21.26 mm2) was larger than that of sham side (1.73 mm2) (p < 0.001). There were significant difference with the sucrose preference (test group: 0.36, control group: 0.76, p < 0.001), duration of immobility (test group: 127.36 s, control group: 59.41 s, p < 0.001), body weight (test group: 156.70 g, control group: 270.06 g, p < 0.001), PMMO (test group: 9.98 mm, control group: 28.79 mm, p < 0.001), CB1 receptor protein (test group: 41.00%, control group: 86.69%, p < 0.001), mean optical density of CB1 receptor protein (test group: 29.60 a.u., control group: 54.69 a.u., p < 0.001), and the number of BrdU-positive cell between the test group and control group (test group: 2133.71, control group: 4301.95, p < 0.001). PMMO was negatively correlated with the duration of immobility (r = 0.953, p < 0.001), while the area of bony fusion was positively correlated (r = 0.961, p < 0.001). PMMO was positively correlated with the sucrose preference, CB1 receptor protein, mean optical density of CB1 receptor protein, and the number of BrdU-positive cell (r = 0.955, 0.955, 0.976, 0.958, p < 0.001, all), while the area of bony fusion was negatively correlated (r = 0.970, 0.981, 0.971, 0.958, p < 0.001, all). Conclusions The present study verified that depressive disorder was found in the rat model of traumatic TMJ bony ankylosis. The severity of TMJ bony ankylosis correlated with the severity of depressive disorder. |
| format | Article |
| id | doaj-art-5befa38f111146da8a2ceb55ed0e724e |
| institution | OA Journals |
| issn | 1472-6831 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | BMC |
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| series | BMC Oral Health |
| spelling | doaj-art-5befa38f111146da8a2ceb55ed0e724e2025-08-20T02:12:02ZengBMCBMC Oral Health1472-68312025-04-0125111410.1186/s12903-025-05886-7The association between traumatic temporomandibular joint bony ankylosis and depressive disorder in growing ratsTiange Deng0Yang Xue1Yiming Wang2Xiang Guo3Zhen Ma4Hongzhi Zhou5Zaiyong Yang6Zhengwu Peng7Xiangxiang Hu8Ning Li9Kaijin Hu10Lei Wang11State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases, Department of Oral and Maxillofacial Surgery, School of Stomatology, The Fourth Military Medical UniversityState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases, Department of Oral and Maxillofacial Surgery, School of Stomatology, The Fourth Military Medical UniversityState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases, Department of Oral and Maxillofacial Surgery, School of Stomatology, The Fourth Military Medical UniversityState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases, Department of Oral and Maxillofacial Surgery, School of Stomatology, The Fourth Military Medical UniversityState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases, Department of Oral and Maxillofacial Surgery, School of Stomatology, The Fourth Military Medical UniversityState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases, Department of Oral and Maxillofacial Surgery, School of Stomatology, The Fourth Military Medical UniversityOral Medicine Teaching and Research Office of the War Rescue Medical Department of the, Noncommissioned Officer Schoolaq, Army Medical UniversityDepartment of Psychiatry, Xijing Hospital, the Fourth Military Medical UniversityDivision of Oral and Craniofacial Health Sciences, University of North Carolina Adams School of DentistrySchool of Stomatology, Jinzhou Medical UniversityDepartment of Stomatology, School of Stomatology, Xi’an Medical University, the Third Affiliated Hospital of Xi’an Medical UniversityDepartment of Female Mental Health, Xi’an Mental Health Center, Xi’an Institute of Mental Health, Shaanxi Mental Health CenterAbstract Background The psychological symptoms of temporomandibular joint (TMJ) ankylosis were similar to that of depressive disorder, but there were no relevant evidences to confirm that the humans or animals with TMJ ankylosis had depressive disorder. The aim of this study was to investigate the association between TMJ ankylosis and depressive disorder in the rat model. Methods Thirty 3-week-old male Sprague–Dawley (SD) rats were used in this study. The damage of TMJ complexes and narrowed joint space were performed in the unilateral TMJ of test group to induce TMJ bony ankylosis (experimental side). The other TMJ of test group underwent a sham operation (sham side). The TMJs of control group did not undergo any operations. At 8 weeks postoperatively, behavioral tests, body weight, passive maximum mouth opening (PMMO), and TMJ morphological features were evaluated, and the hippocampuses were analyzed using western blotting and immunocytochemistry. The data was compared between the test group and control group by independent t-test, between the experimental side and sham side by paired t-test. The correlations between PMMO/area of bony fusion and duration of immobility, sucrose preference, CB1 receptor protein, mean optical density of CB1 receptor protein, and the number of BrdU-positive cell were evaluated using linear regression analysis. The level of significance was 0.05. Results In the test group, the traumatic TMJ complexes with narrowed joint space developed TMJ bony ankylosis, the area of bony mass of experimental side (21.26 mm2) was larger than that of sham side (1.73 mm2) (p < 0.001). There were significant difference with the sucrose preference (test group: 0.36, control group: 0.76, p < 0.001), duration of immobility (test group: 127.36 s, control group: 59.41 s, p < 0.001), body weight (test group: 156.70 g, control group: 270.06 g, p < 0.001), PMMO (test group: 9.98 mm, control group: 28.79 mm, p < 0.001), CB1 receptor protein (test group: 41.00%, control group: 86.69%, p < 0.001), mean optical density of CB1 receptor protein (test group: 29.60 a.u., control group: 54.69 a.u., p < 0.001), and the number of BrdU-positive cell between the test group and control group (test group: 2133.71, control group: 4301.95, p < 0.001). PMMO was negatively correlated with the duration of immobility (r = 0.953, p < 0.001), while the area of bony fusion was positively correlated (r = 0.961, p < 0.001). PMMO was positively correlated with the sucrose preference, CB1 receptor protein, mean optical density of CB1 receptor protein, and the number of BrdU-positive cell (r = 0.955, 0.955, 0.976, 0.958, p < 0.001, all), while the area of bony fusion was negatively correlated (r = 0.970, 0.981, 0.971, 0.958, p < 0.001, all). Conclusions The present study verified that depressive disorder was found in the rat model of traumatic TMJ bony ankylosis. The severity of TMJ bony ankylosis correlated with the severity of depressive disorder.https://doi.org/10.1186/s12903-025-05886-7TMJ ankylosisMaximum mouth openingArea of bony fusionDepressive disorderSucrose preferenceDuration of immobility |
| spellingShingle | Tiange Deng Yang Xue Yiming Wang Xiang Guo Zhen Ma Hongzhi Zhou Zaiyong Yang Zhengwu Peng Xiangxiang Hu Ning Li Kaijin Hu Lei Wang The association between traumatic temporomandibular joint bony ankylosis and depressive disorder in growing rats BMC Oral Health TMJ ankylosis Maximum mouth opening Area of bony fusion Depressive disorder Sucrose preference Duration of immobility |
| title | The association between traumatic temporomandibular joint bony ankylosis and depressive disorder in growing rats |
| title_full | The association between traumatic temporomandibular joint bony ankylosis and depressive disorder in growing rats |
| title_fullStr | The association between traumatic temporomandibular joint bony ankylosis and depressive disorder in growing rats |
| title_full_unstemmed | The association between traumatic temporomandibular joint bony ankylosis and depressive disorder in growing rats |
| title_short | The association between traumatic temporomandibular joint bony ankylosis and depressive disorder in growing rats |
| title_sort | association between traumatic temporomandibular joint bony ankylosis and depressive disorder in growing rats |
| topic | TMJ ankylosis Maximum mouth opening Area of bony fusion Depressive disorder Sucrose preference Duration of immobility |
| url | https://doi.org/10.1186/s12903-025-05886-7 |
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