Exploration of the primary antibiofilm substance and mechanism employed by Lactobacillus salivarius ATCC 11741 to inhibit biofilm of Streptococcus mutans
IntroductionLactobacillus salivarius serves as a probiotic potentially capable of preventing dental caries both in vitro and in vivo. This study focused on understanding the key antibiofilm agents and the mechanisms of action of the Lactobacilli supernatant against Streptococcus mutans.MethodsStrept...
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Frontiers Media S.A.
2025-03-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1535539/full |
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| author | Nan Ma Nan Ma Wei Yang Wei Yang Bairu Chen Bairu Chen Meihua Bao Meihua Bao Yimin Li Yimin Li Meng Wang Meng Wang Xiaopeng Yang Xiaopeng Yang Junyi Liu Chengyue Wang Chengyue Wang Lihong Qiu |
| author_facet | Nan Ma Nan Ma Wei Yang Wei Yang Bairu Chen Bairu Chen Meihua Bao Meihua Bao Yimin Li Yimin Li Meng Wang Meng Wang Xiaopeng Yang Xiaopeng Yang Junyi Liu Chengyue Wang Chengyue Wang Lihong Qiu |
| author_sort | Nan Ma |
| collection | DOAJ |
| description | IntroductionLactobacillus salivarius serves as a probiotic potentially capable of preventing dental caries both in vitro and in vivo. This study focused on understanding the key antibiofilm agents and the mechanisms of action of the Lactobacilli supernatant against Streptococcus mutans.MethodsStreptococcus mutans biofilm was constructed and the cell-free supernatant of Lactobacillus salivarius was added. After the biofilm was collected, RNA-seq and qRT-PCR were then performed to get gene information. The influence of temperature, pH and other factors on the supernatant were measured and non-targeted metabolome analysis was performed to analyze the effective components.ResultsThe findings indicated that the supernatant derived from Lactobacillus salivarius could inhibit the biofilm formation of Streptococcus mutans at different times. Through transcriptome analysis, we discovered that the cell-free supernatant reduced biofilm formation, by suppressing phosphoenolpyruvate-dependent phosphotransferase systems along with two ATP-binding cassette transporters, rather than directly affecting the genes that code for glucosyltransferases; additionally, the supernatant was observed to diminish the expression of genes linked to two-component systems, polyketides/non-ribosomal peptides, acid stress response, quorum sensing, and exopolysaccharide formation. Non-targeted LC-MS/MS analysis was employed to discover a variety of potential active compounds present in the cellular filtrate of Lactobacillus salivarius that hinder the growth of S. mutans, including phenyllactic acid, sorbitol, and honokiol.DiscussionIn summary, our findings support the evaluation of Lactobacillus salivarius as a promising oral probiotic aimed at hindering the formation of biofilms by cariogenic pathogens and the development of dental caries. |
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| id | doaj-art-5beb730c01974de98731d0a997f74484 |
| institution | DOAJ |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-5beb730c01974de98731d0a997f744842025-08-20T02:47:45ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-03-011510.3389/fcimb.2025.15355391535539Exploration of the primary antibiofilm substance and mechanism employed by Lactobacillus salivarius ATCC 11741 to inhibit biofilm of Streptococcus mutansNan Ma0Nan Ma1Wei Yang2Wei Yang3Bairu Chen4Bairu Chen5Meihua Bao6Meihua Bao7Yimin Li8Yimin Li9Meng Wang10Meng Wang11Xiaopeng Yang12Xiaopeng Yang13Junyi Liu14Chengyue Wang15Chengyue Wang16Lihong Qiu17Department of Periodontics, Affiliated Stomatology Hospital of Jinzhou Medical University, Jinzhou, ChinaCollaborative Innovation Center for Health Promotion of Children and Adolescents of Jinzhou Medical University, Jinzhou, ChinaCollaborative Innovation Center for Health Promotion of Children and Adolescents of Jinzhou Medical University, Jinzhou, ChinaDepartment of Pedodontics, Affiliated Stomatology Hospital of Jinzhou Medical University, Jinzhou, ChinaCollaborative Innovation Center for Health Promotion of Children and Adolescents of Jinzhou Medical University, Jinzhou, ChinaDepartment of Prosthetics, Affiliated Stomatology Hospital of Jinzhou Medical University, Jinzhou, ChinaCollaborative Innovation Center for Health Promotion of Children and Adolescents of Jinzhou Medical University, Jinzhou, ChinaDepartment of Prosthetics, Affiliated Stomatology Hospital of Jinzhou Medical University, Jinzhou, ChinaCollaborative Innovation Center for Health Promotion of Children and Adolescents of Jinzhou Medical University, Jinzhou, ChinaDepartment of Prosthetics, Affiliated Stomatology Hospital of Jinzhou Medical University, Jinzhou, ChinaCollaborative Innovation Center for Health Promotion of Children and Adolescents of Jinzhou Medical University, Jinzhou, ChinaDepartment of Prosthetics, Affiliated Stomatology Hospital of Jinzhou Medical University, Jinzhou, ChinaDepartment of Pedodontics, Affiliated Stomatology Hospital of Jinzhou Medical University, Jinzhou, ChinaCollaborative Innovation Center for Health Promotion of Children and Adolescents of Jinzhou Medical University, Jinzhou, ChinaJinzhou Medical University, Jinzhou, ChinaCollaborative Innovation Center for Health Promotion of Children and Adolescents of Jinzhou Medical University, Jinzhou, ChinaDepartment of Prosthetics, Affiliated Stomatology Hospital of Jinzhou Medical University, Jinzhou, ChinaDepartment of Endodontics, School and Hospital of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases, Shenyang, ChinaIntroductionLactobacillus salivarius serves as a probiotic potentially capable of preventing dental caries both in vitro and in vivo. This study focused on understanding the key antibiofilm agents and the mechanisms of action of the Lactobacilli supernatant against Streptococcus mutans.MethodsStreptococcus mutans biofilm was constructed and the cell-free supernatant of Lactobacillus salivarius was added. After the biofilm was collected, RNA-seq and qRT-PCR were then performed to get gene information. The influence of temperature, pH and other factors on the supernatant were measured and non-targeted metabolome analysis was performed to analyze the effective components.ResultsThe findings indicated that the supernatant derived from Lactobacillus salivarius could inhibit the biofilm formation of Streptococcus mutans at different times. Through transcriptome analysis, we discovered that the cell-free supernatant reduced biofilm formation, by suppressing phosphoenolpyruvate-dependent phosphotransferase systems along with two ATP-binding cassette transporters, rather than directly affecting the genes that code for glucosyltransferases; additionally, the supernatant was observed to diminish the expression of genes linked to two-component systems, polyketides/non-ribosomal peptides, acid stress response, quorum sensing, and exopolysaccharide formation. Non-targeted LC-MS/MS analysis was employed to discover a variety of potential active compounds present in the cellular filtrate of Lactobacillus salivarius that hinder the growth of S. mutans, including phenyllactic acid, sorbitol, and honokiol.DiscussionIn summary, our findings support the evaluation of Lactobacillus salivarius as a promising oral probiotic aimed at hindering the formation of biofilms by cariogenic pathogens and the development of dental caries.https://www.frontiersin.org/articles/10.3389/fcimb.2025.1535539/fullStreptococcus mutanstranscriptomicsmetabolomicsbiofilmdental cariesLactobacillus salivarius |
| spellingShingle | Nan Ma Nan Ma Wei Yang Wei Yang Bairu Chen Bairu Chen Meihua Bao Meihua Bao Yimin Li Yimin Li Meng Wang Meng Wang Xiaopeng Yang Xiaopeng Yang Junyi Liu Chengyue Wang Chengyue Wang Lihong Qiu Exploration of the primary antibiofilm substance and mechanism employed by Lactobacillus salivarius ATCC 11741 to inhibit biofilm of Streptococcus mutans Frontiers in Cellular and Infection Microbiology Streptococcus mutans transcriptomics metabolomics biofilm dental caries Lactobacillus salivarius |
| title | Exploration of the primary antibiofilm substance and mechanism employed by Lactobacillus salivarius ATCC 11741 to inhibit biofilm of Streptococcus mutans |
| title_full | Exploration of the primary antibiofilm substance and mechanism employed by Lactobacillus salivarius ATCC 11741 to inhibit biofilm of Streptococcus mutans |
| title_fullStr | Exploration of the primary antibiofilm substance and mechanism employed by Lactobacillus salivarius ATCC 11741 to inhibit biofilm of Streptococcus mutans |
| title_full_unstemmed | Exploration of the primary antibiofilm substance and mechanism employed by Lactobacillus salivarius ATCC 11741 to inhibit biofilm of Streptococcus mutans |
| title_short | Exploration of the primary antibiofilm substance and mechanism employed by Lactobacillus salivarius ATCC 11741 to inhibit biofilm of Streptococcus mutans |
| title_sort | exploration of the primary antibiofilm substance and mechanism employed by lactobacillus salivarius atcc 11741 to inhibit biofilm of streptococcus mutans |
| topic | Streptococcus mutans transcriptomics metabolomics biofilm dental caries Lactobacillus salivarius |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1535539/full |
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