Spontaneous Bio-Recycling: Recovering Bioactive Molecules Through Endogenous Microbial Maceration of Hemp Residues
Biomass residues represent a major issue for industries. On the other hand, residues enclosed major classes of bioactive compounds that could be extracted and used across various fields. This study aimed to elucidate the role of the endogenous microbial community in the lignocellulosic degradation o...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-02-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/13/2/455 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850081208546885632 |
|---|---|
| author | Leonardo Donati Angela Conti Debora Casagrande Pierantoni Chiara Ruspi Martina Cerri Carla Emiliani Gianluigi Cardinali Laura Corte |
| author_facet | Leonardo Donati Angela Conti Debora Casagrande Pierantoni Chiara Ruspi Martina Cerri Carla Emiliani Gianluigi Cardinali Laura Corte |
| author_sort | Leonardo Donati |
| collection | DOAJ |
| description | Biomass residues represent a major issue for industries. On the other hand, residues enclosed major classes of bioactive compounds that could be extracted and used across various fields. This study aimed to elucidate the role of the endogenous microbial community in the lignocellulosic degradation of hemp residues for biorefineries or other industrial processes, simultaneously characterizing the composition of three extracts recovered at different stages of maceration. The process was examined from different perspectives: plant tissue degradation and microbial dynamics were monitored using histological, cultural-dependent, and independent analysis. Extracts were characterized through FTIR, NMR, and SDS-PAGE analyses, also evaluating their potential as biostimulants for microbial growth. Results revealed that the composition of the endogenous prokaryotic community remained stable during the maceration period, while fluctuations in various fungal genera were observed. The taxonomical composition of hemp residues at different stages may account for the increased accumulation of amide-containing compounds, sugars, and other metabolites detected in long-term bioconversion. Finally, the extracts recovered from the microbial degradation of hemp residues were able to support the growth of the yeast <i>Cryptococcus phenolicus</i> and the bacterium <i>Peribacillus simplex</i> as the sole source of nitrogen, paving the way for their potential use as biostimulants. |
| format | Article |
| id | doaj-art-5bdfb9c0adae449988cf154c6ae930e1 |
| institution | DOAJ |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-5bdfb9c0adae449988cf154c6ae930e12025-08-20T02:44:47ZengMDPI AGMicroorganisms2076-26072025-02-0113245510.3390/microorganisms13020455Spontaneous Bio-Recycling: Recovering Bioactive Molecules Through Endogenous Microbial Maceration of Hemp ResiduesLeonardo Donati0Angela Conti1Debora Casagrande Pierantoni2Chiara Ruspi3Martina Cerri4Carla Emiliani5Gianluigi Cardinali6Laura Corte7Department of Chemistry, Biology and Biotechnology, University of Perugia, 06126 Perugia, ItalyDepartment of Pharmaceutical Sciences, University of Perugia, 06126 Perugia, ItalyDepartment of Pharmaceutical Sciences, University of Perugia, 06126 Perugia, ItalyDepartment of Pharmaceutical Sciences, University of Perugia, 06126 Perugia, ItalyDepartment of Agricultural, Food and Environmental Sciences, University of Perugia, 06126 Perugia, ItalyDepartment of Chemistry, Biology and Biotechnology, University of Perugia, 06126 Perugia, ItalyDepartment of Pharmaceutical Sciences, University of Perugia, 06126 Perugia, ItalyDepartment of Pharmaceutical Sciences, University of Perugia, 06126 Perugia, ItalyBiomass residues represent a major issue for industries. On the other hand, residues enclosed major classes of bioactive compounds that could be extracted and used across various fields. This study aimed to elucidate the role of the endogenous microbial community in the lignocellulosic degradation of hemp residues for biorefineries or other industrial processes, simultaneously characterizing the composition of three extracts recovered at different stages of maceration. The process was examined from different perspectives: plant tissue degradation and microbial dynamics were monitored using histological, cultural-dependent, and independent analysis. Extracts were characterized through FTIR, NMR, and SDS-PAGE analyses, also evaluating their potential as biostimulants for microbial growth. Results revealed that the composition of the endogenous prokaryotic community remained stable during the maceration period, while fluctuations in various fungal genera were observed. The taxonomical composition of hemp residues at different stages may account for the increased accumulation of amide-containing compounds, sugars, and other metabolites detected in long-term bioconversion. Finally, the extracts recovered from the microbial degradation of hemp residues were able to support the growth of the yeast <i>Cryptococcus phenolicus</i> and the bacterium <i>Peribacillus simplex</i> as the sole source of nitrogen, paving the way for their potential use as biostimulants.https://www.mdpi.com/2076-2607/13/2/455<i>Cannabis sativa</i> L.microbiotasustainabilitymacerationmetabarcodingextracts |
| spellingShingle | Leonardo Donati Angela Conti Debora Casagrande Pierantoni Chiara Ruspi Martina Cerri Carla Emiliani Gianluigi Cardinali Laura Corte Spontaneous Bio-Recycling: Recovering Bioactive Molecules Through Endogenous Microbial Maceration of Hemp Residues Microorganisms <i>Cannabis sativa</i> L. microbiota sustainability maceration metabarcoding extracts |
| title | Spontaneous Bio-Recycling: Recovering Bioactive Molecules Through Endogenous Microbial Maceration of Hemp Residues |
| title_full | Spontaneous Bio-Recycling: Recovering Bioactive Molecules Through Endogenous Microbial Maceration of Hemp Residues |
| title_fullStr | Spontaneous Bio-Recycling: Recovering Bioactive Molecules Through Endogenous Microbial Maceration of Hemp Residues |
| title_full_unstemmed | Spontaneous Bio-Recycling: Recovering Bioactive Molecules Through Endogenous Microbial Maceration of Hemp Residues |
| title_short | Spontaneous Bio-Recycling: Recovering Bioactive Molecules Through Endogenous Microbial Maceration of Hemp Residues |
| title_sort | spontaneous bio recycling recovering bioactive molecules through endogenous microbial maceration of hemp residues |
| topic | <i>Cannabis sativa</i> L. microbiota sustainability maceration metabarcoding extracts |
| url | https://www.mdpi.com/2076-2607/13/2/455 |
| work_keys_str_mv | AT leonardodonati spontaneousbiorecyclingrecoveringbioactivemoleculesthroughendogenousmicrobialmacerationofhempresidues AT angelaconti spontaneousbiorecyclingrecoveringbioactivemoleculesthroughendogenousmicrobialmacerationofhempresidues AT deboracasagrandepierantoni spontaneousbiorecyclingrecoveringbioactivemoleculesthroughendogenousmicrobialmacerationofhempresidues AT chiararuspi spontaneousbiorecyclingrecoveringbioactivemoleculesthroughendogenousmicrobialmacerationofhempresidues AT martinacerri spontaneousbiorecyclingrecoveringbioactivemoleculesthroughendogenousmicrobialmacerationofhempresidues AT carlaemiliani spontaneousbiorecyclingrecoveringbioactivemoleculesthroughendogenousmicrobialmacerationofhempresidues AT gianluigicardinali spontaneousbiorecyclingrecoveringbioactivemoleculesthroughendogenousmicrobialmacerationofhempresidues AT lauracorte spontaneousbiorecyclingrecoveringbioactivemoleculesthroughendogenousmicrobialmacerationofhempresidues |