Prolactin Rescues Immature B-Cells from Apoptosis Induced by B-Cell Receptor Cross-Linking
Prolactin has an immunomodulatory effect and has been associated with B-cell-triggered autoimmune diseases, such as systemic lupus erythematosus (SLE). In mice that develop SLE, the PRL receptor is expressed in early bone marrow B-cells, and increased levels of PRL hasten disease manifestations, whi...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/3219017 |
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| author | Rocio Flores-Fernández Francisco Blanco-Favela Ezequiel M. Fuentes-Pananá Luis Chávez-Sánchez Patricia Gorocica-Rosete Alberto Pizaña-Venegas Adriana Karina Chávez-Rueda |
| author_facet | Rocio Flores-Fernández Francisco Blanco-Favela Ezequiel M. Fuentes-Pananá Luis Chávez-Sánchez Patricia Gorocica-Rosete Alberto Pizaña-Venegas Adriana Karina Chávez-Rueda |
| author_sort | Rocio Flores-Fernández |
| collection | DOAJ |
| description | Prolactin has an immunomodulatory effect and has been associated with B-cell-triggered autoimmune diseases, such as systemic lupus erythematosus (SLE). In mice that develop SLE, the PRL receptor is expressed in early bone marrow B-cells, and increased levels of PRL hasten disease manifestations, which are correlated with a reduction in the absolute number of immature B-cells. The aim of this work was to determine the effect of PRL in an in vitro system of B-cell tolerance using WEHI-231 cells and immature B-cells from lupus prone MRL/lpr mice. WEHI-231 cells express the long isoform of the PRL receptor, and PRL rescued the cells from cell death by decreasing the apoptosis induced by the cross-linking of the B-cell antigen receptor (BCR) as measured by Annexin V and active caspase-3. This decrease in apoptosis may have been due to the PRL and receptor interaction, which increased the relative expression of antiapoptotic Bcl-xL and decreased the relative expression of proapoptotic Bad. In immature B-cells from MRL/lpr mice, PRL increased the viability and decreased the apoptosis induced by the cross-linking of BCR, which may favor the maturation of self-reactive B-cells and contribute to the onset of disease. |
| format | Article |
| id | doaj-art-5bddc6dd75984eddbc176dab46f86d7c |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-5bddc6dd75984eddbc176dab46f86d7c2025-08-20T03:34:28ZengWileyJournal of Immunology Research2314-88612314-71562016-01-01201610.1155/2016/32190173219017Prolactin Rescues Immature B-Cells from Apoptosis Induced by B-Cell Receptor Cross-LinkingRocio Flores-Fernández0Francisco Blanco-Favela1Ezequiel M. Fuentes-Pananá2Luis Chávez-Sánchez3Patricia Gorocica-Rosete4Alberto Pizaña-Venegas5Adriana Karina Chávez-Rueda6UIM en Inmunología, Hospital de Pediatría, CMN Siglo XXI, IMSS, 06720 Ciudad de México, DF, MexicoUIM en Inmunología, Hospital de Pediatría, CMN Siglo XXI, IMSS, 06720 Ciudad de México, DF, MexicoHospital Infantil de México Federico Gómez, Unidad de Investigación en Virología y Cáncer, 06720 Ciudad de México, DF, MexicoUIM en Inmunología, Hospital de Pediatría, CMN Siglo XXI, IMSS, 06720 Ciudad de México, DF, MexicoDepartamento de Investigación en Bioquímica, Instituto Nacional de Enfermedades Respiratorias “Ismael Cosió Villegas”, 14080 Ciudad de México, DF, MexicoUnidad de Investigación y Bioterio, Instituto Nacional de Enfermedades Respiratorias “Ismael Cosió Villegas”, 14080 Ciudad de México, DF, MexicoUIM en Inmunología, Hospital de Pediatría, CMN Siglo XXI, IMSS, 06720 Ciudad de México, DF, MexicoProlactin has an immunomodulatory effect and has been associated with B-cell-triggered autoimmune diseases, such as systemic lupus erythematosus (SLE). In mice that develop SLE, the PRL receptor is expressed in early bone marrow B-cells, and increased levels of PRL hasten disease manifestations, which are correlated with a reduction in the absolute number of immature B-cells. The aim of this work was to determine the effect of PRL in an in vitro system of B-cell tolerance using WEHI-231 cells and immature B-cells from lupus prone MRL/lpr mice. WEHI-231 cells express the long isoform of the PRL receptor, and PRL rescued the cells from cell death by decreasing the apoptosis induced by the cross-linking of the B-cell antigen receptor (BCR) as measured by Annexin V and active caspase-3. This decrease in apoptosis may have been due to the PRL and receptor interaction, which increased the relative expression of antiapoptotic Bcl-xL and decreased the relative expression of proapoptotic Bad. In immature B-cells from MRL/lpr mice, PRL increased the viability and decreased the apoptosis induced by the cross-linking of BCR, which may favor the maturation of self-reactive B-cells and contribute to the onset of disease.http://dx.doi.org/10.1155/2016/3219017 |
| spellingShingle | Rocio Flores-Fernández Francisco Blanco-Favela Ezequiel M. Fuentes-Pananá Luis Chávez-Sánchez Patricia Gorocica-Rosete Alberto Pizaña-Venegas Adriana Karina Chávez-Rueda Prolactin Rescues Immature B-Cells from Apoptosis Induced by B-Cell Receptor Cross-Linking Journal of Immunology Research |
| title | Prolactin Rescues Immature B-Cells from Apoptosis Induced by B-Cell Receptor Cross-Linking |
| title_full | Prolactin Rescues Immature B-Cells from Apoptosis Induced by B-Cell Receptor Cross-Linking |
| title_fullStr | Prolactin Rescues Immature B-Cells from Apoptosis Induced by B-Cell Receptor Cross-Linking |
| title_full_unstemmed | Prolactin Rescues Immature B-Cells from Apoptosis Induced by B-Cell Receptor Cross-Linking |
| title_short | Prolactin Rescues Immature B-Cells from Apoptosis Induced by B-Cell Receptor Cross-Linking |
| title_sort | prolactin rescues immature b cells from apoptosis induced by b cell receptor cross linking |
| url | http://dx.doi.org/10.1155/2016/3219017 |
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