Development of IC-RT real-time PCR method for detection of Cowpea severe mosaic virus
A method of immuno-capture reverse transcript real-time (IC-RT real-time) PCR assay was developed for detection of Cow pea severe mosaic virus (CPSMV). By introducing standard CPSMV strains from ATCC and DSMZ as materials, RT real-time PCR method was constituted firstly, which showed good amplificat...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2010-09-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2010.05.003 |
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| _version_ | 1850044874851614720 |
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| author | LI Bin SU Han WU Cui-ping ZHOU Ming-hua AN Yu-lin |
| author_facet | LI Bin SU Han WU Cui-ping ZHOU Ming-hua AN Yu-lin |
| author_sort | LI Bin |
| collection | DOAJ |
| description | A method of immuno-capture reverse transcript real-time (IC-RT real-time) PCR assay was developed for detection of Cow pea severe mosaic virus (CPSMV). By introducing standard CPSMV strains from ATCC and DSMZ as materials, RT real-time PCR method was constituted firstly, which showed good amplification reaction and specificity. Based on the RT real-time PCR, IC-RT real-time PCR detection method for CPSMV was set up. The sensitivity of the assay could reach 500 pg leaf tissues. Meanwhile, the IC-RT real-time PCR assay was characteristic of good specificity, high sensitivity, convenient operation, and avoided RNA extraction, which was suitable for rapid detection of CPSMV from legume seeds and seedlings such as cowpea. |
| format | Article |
| id | doaj-art-5bd29afc6ab742a7ab79672f7429ed2a |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2010-09-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-5bd29afc6ab742a7ab79672f7429ed2a2025-08-20T02:54:50ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552010-09-013649149610.3785/j.issn.1008-9209.2010.05.00310089209Development of IC-RT real-time PCR method for detection of Cowpea severe mosaic virusLI BinSU HanWU Cui-pingZHOU Ming-huaAN Yu-linA method of immuno-capture reverse transcript real-time (IC-RT real-time) PCR assay was developed for detection of Cow pea severe mosaic virus (CPSMV). By introducing standard CPSMV strains from ATCC and DSMZ as materials, RT real-time PCR method was constituted firstly, which showed good amplification reaction and specificity. Based on the RT real-time PCR, IC-RT real-time PCR detection method for CPSMV was set up. The sensitivity of the assay could reach 500 pg leaf tissues. Meanwhile, the IC-RT real-time PCR assay was characteristic of good specificity, high sensitivity, convenient operation, and avoided RNA extraction, which was suitable for rapid detection of CPSMV from legume seeds and seedlings such as cowpea.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2010.05.003<italic>Cow pea severe mosaic virus</italic>immuno-capture reverse transcriptreal-time PCR |
| spellingShingle | LI Bin SU Han WU Cui-ping ZHOU Ming-hua AN Yu-lin Development of IC-RT real-time PCR method for detection of Cowpea severe mosaic virus 浙江大学学报. 农业与生命科学版 <italic>Cow pea severe mosaic virus</italic> immuno-capture reverse transcript real-time PCR |
| title | Development of IC-RT real-time PCR method for detection of Cowpea severe mosaic virus |
| title_full | Development of IC-RT real-time PCR method for detection of Cowpea severe mosaic virus |
| title_fullStr | Development of IC-RT real-time PCR method for detection of Cowpea severe mosaic virus |
| title_full_unstemmed | Development of IC-RT real-time PCR method for detection of Cowpea severe mosaic virus |
| title_short | Development of IC-RT real-time PCR method for detection of Cowpea severe mosaic virus |
| title_sort | development of ic rt real time pcr method for detection of cowpea severe mosaic virus |
| topic | <italic>Cow pea severe mosaic virus</italic> immuno-capture reverse transcript real-time PCR |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2010.05.003 |
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