The effects of oleuropein on lung and heart injury in cecal ligation and puncture - induced sepsis
<b>Aim:</b> Sepsis is a fatal serious clinical disorder. The oxidative stress in sepsis plays an important role in the inflammation. Therefore, the effects of oleuropein (OLE) and ceftriaxone (CEFT) on sepsis were investigated in this study.<p> <b>Materials and Methods:</b...
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| Format: | Article |
| Language: | English |
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Selcuk University Press
2020-10-01
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| Series: | Eurasian Journal of Veterinary Sciences |
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| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1301 |
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| author | Hüseyin Serkan Erol Tuba Aydın Serkan Yıldırım İsmail Can Mesut Bünyami Halıcı |
| author_facet | Hüseyin Serkan Erol Tuba Aydın Serkan Yıldırım İsmail Can Mesut Bünyami Halıcı |
| author_sort | Hüseyin Serkan Erol |
| collection | DOAJ |
| description | <b>Aim:</b> Sepsis is a fatal serious clinical disorder. The oxidative stress
in sepsis plays an important role in the inflammation. Therefore, the
effects of oleuropein (OLE) and ceftriaxone (CEFT) on sepsis were
investigated in this study.<p>
<b>Materials and Methods:</b> Thirty male rats were divided into five
equal groups: Sham, Sepsis, CEFT, OLE-150 and OLE-300, The
150 mg/kg of ceftriaxone (i.p.), and 150 and 300 mg/kg OLE (p.o.)
were administered to the treatment groups fifteen minutes before
the experiment. After 24 hours, blood samples, lungs and hearts tissues
were taken. The creatine kinase (CK) and lactate dehydrogenase
(LDH) activities in serum, lipid peroxidation (LPO), glutathione
(GSH), superoxide dismutase (SOD) and catalase (CAT) levels were
all determined. In addition, the expressions of the interleukin (IL)-8
in lung and tumor necrosis factor (TNF)-? in heart were pathologically
and immunohistochemically were determined.<p>
<b>Results:</b> Sepsis caused significant increases in CK, LDH, LPO, GSH
and heart CAT activity and caused inhibition of SOD and lung CAT
activity (p<0.001). The OLE significantly decreased the LPO and GSH
levels (p<0.001) and increased the SOD in activity of both tissues
and CAT activity in lung tissue (p<0.001). Moreover, CEFT significantly
decreased the LPO level (p<0.01) and increased tissue SOD
and CAT activities in lung tissue (p<0.001). CK and LDH activities in
serum showed marked decrease in OLE and CEFT groups (p<0.001).<p>
<b>Conclusion:</b> Antioxidant and a notable anti-inflammatory effect of
OLE by reducing IL-8 and TNF-? levels have been stated. Thus, a
strong antioxidant, such as OLE, would be beneficial for the treatment
of sepsis. |
| format | Article |
| id | doaj-art-5bd1c5f5d752480da6f6913510611c4a |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publishDate | 2020-10-01 |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-5bd1c5f5d752480da6f6913510611c4a2025-01-03T01:36:24ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19532020-10-013632212311301The effects of oleuropein on lung and heart injury in cecal ligation and puncture - induced sepsisHüseyin Serkan Erol0Tuba Aydın1Serkan Yıldırım2İsmail Can3Mesut Bünyami Halıcı4Kastamonu University, Veterinary Faculty, Department of Biochemistry, Kastamonu, TurkeyAgri Ibrahim Cecen University, Pharmacy Faculty, Department of Pharmacognosy, Agri, TurkeyAtaturk University, Veterinary Faculty, Department of Pathology, Erzurum, TurkeyKafkas University, Medicine Faculty, Department of Histology and Embriology, Kars, TurkeyAtaturk University, Veterinary Faculty, Department of Biochemistry, Erzurum, Turkey<b>Aim:</b> Sepsis is a fatal serious clinical disorder. The oxidative stress in sepsis plays an important role in the inflammation. Therefore, the effects of oleuropein (OLE) and ceftriaxone (CEFT) on sepsis were investigated in this study.<p> <b>Materials and Methods:</b> Thirty male rats were divided into five equal groups: Sham, Sepsis, CEFT, OLE-150 and OLE-300, The 150 mg/kg of ceftriaxone (i.p.), and 150 and 300 mg/kg OLE (p.o.) were administered to the treatment groups fifteen minutes before the experiment. After 24 hours, blood samples, lungs and hearts tissues were taken. The creatine kinase (CK) and lactate dehydrogenase (LDH) activities in serum, lipid peroxidation (LPO), glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) levels were all determined. In addition, the expressions of the interleukin (IL)-8 in lung and tumor necrosis factor (TNF)-? in heart were pathologically and immunohistochemically were determined.<p> <b>Results:</b> Sepsis caused significant increases in CK, LDH, LPO, GSH and heart CAT activity and caused inhibition of SOD and lung CAT activity (p<0.001). The OLE significantly decreased the LPO and GSH levels (p<0.001) and increased the SOD in activity of both tissues and CAT activity in lung tissue (p<0.001). Moreover, CEFT significantly decreased the LPO level (p<0.01) and increased tissue SOD and CAT activities in lung tissue (p<0.001). CK and LDH activities in serum showed marked decrease in OLE and CEFT groups (p<0.001).<p> <b>Conclusion:</b> Antioxidant and a notable anti-inflammatory effect of OLE by reducing IL-8 and TNF-? levels have been stated. Thus, a strong antioxidant, such as OLE, would be beneficial for the treatment of sepsis.http://eurasianjvetsci.org/pdf.php3?id=1301oleuropeinsepsis modelceftriaxoneinflammation |
| spellingShingle | Hüseyin Serkan Erol Tuba Aydın Serkan Yıldırım İsmail Can Mesut Bünyami Halıcı The effects of oleuropein on lung and heart injury in cecal ligation and puncture - induced sepsis Eurasian Journal of Veterinary Sciences oleuropein sepsis model ceftriaxone inflammation |
| title | The effects of oleuropein on lung and heart injury in cecal ligation and puncture - induced sepsis |
| title_full | The effects of oleuropein on lung and heart injury in cecal ligation and puncture - induced sepsis |
| title_fullStr | The effects of oleuropein on lung and heart injury in cecal ligation and puncture - induced sepsis |
| title_full_unstemmed | The effects of oleuropein on lung and heart injury in cecal ligation and puncture - induced sepsis |
| title_short | The effects of oleuropein on lung and heart injury in cecal ligation and puncture - induced sepsis |
| title_sort | effects of oleuropein on lung and heart injury in cecal ligation and puncture induced sepsis |
| topic | oleuropein sepsis model ceftriaxone inflammation |
| url | http://eurasianjvetsci.org/pdf.php3?id=1301 |
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