Draft Genome Resource of a Wolbachia Endosymbiont in Heterodera humuli
The bacterial endosymbiont Wolbachia is known to be associated with different arthropods and only two genera of plant-parasitic nematodes, Pratylenchus and Radopholus. This effort employed a genome skimming approach to discover the presence of endosymbionts in a population of Heterodera humuli seque...
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The American Phytopathological Society
2024-12-01
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| Series: | PhytoFrontiers |
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| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-05-24-0054-A |
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| author | Lester A. Núñez-Rodríguez Catherine L. Wram Cedar Hesse Inga A. Zasada |
| author_facet | Lester A. Núñez-Rodríguez Catherine L. Wram Cedar Hesse Inga A. Zasada |
| author_sort | Lester A. Núñez-Rodríguez |
| collection | DOAJ |
| description | The bacterial endosymbiont Wolbachia is known to be associated with different arthropods and only two genera of plant-parasitic nematodes, Pratylenchus and Radopholus. This effort employed a genome skimming approach to discover the presence of endosymbionts in a population of Heterodera humuli sequenced with PacBio long-read sequencing. Wolbachia was found associated with the nematode. The genome of this Wolbachia is 1,051,007 bp and has a GC percentage (32.6%) within the expected range for the genus. A phylogenetic analysis placed the Wolbachia strain from H. humuli in a clade with another nematode-associated Wolbachia strain reported in Texas, with a bootstrap value of 1. To our knowledge, this is the first published report of Wolbachia associated with H. humuli, expanding the known association of this endosymbiont to three genera of plant-parasitic nematodes. This finding will enhance sequence resources for further comparisons of Wolbachia diversity. [Figure: see text] The author(s) have dedicated the work to the public domain under the Creative Commons CC0 “No Rights Reserved” license by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law, 2024. |
| format | Article |
| id | doaj-art-5bc4aee464174d30b512bd05412fea31 |
| institution | DOAJ |
| issn | 2690-5442 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | PhytoFrontiers |
| spelling | doaj-art-5bc4aee464174d30b512bd05412fea312025-08-20T02:39:15ZengThe American Phytopathological SocietyPhytoFrontiers2690-54422024-12-014483283610.1094/PHYTOFR-05-24-0054-ADraft Genome Resource of a Wolbachia Endosymbiont in Heterodera humuliLester A. Núñez-Rodríguez0Catherine L. Wram1Cedar Hesse2Inga A. Zasada3Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331Mycology and Nematology Genetic Diversity and Biology Lab, USDA-ARS, Beltsville, MD 20705Horticultural Crops Production and Genetic Improvement Research Unit, USDA-ARS, Corvallis, OR 97331Horticultural Crops Disease and Pest Management Research Unit, United States Department of Agriculture (USDA)-Agricultural Research Service (ARS), Corvallis, OR 97331The bacterial endosymbiont Wolbachia is known to be associated with different arthropods and only two genera of plant-parasitic nematodes, Pratylenchus and Radopholus. This effort employed a genome skimming approach to discover the presence of endosymbionts in a population of Heterodera humuli sequenced with PacBio long-read sequencing. Wolbachia was found associated with the nematode. The genome of this Wolbachia is 1,051,007 bp and has a GC percentage (32.6%) within the expected range for the genus. A phylogenetic analysis placed the Wolbachia strain from H. humuli in a clade with another nematode-associated Wolbachia strain reported in Texas, with a bootstrap value of 1. To our knowledge, this is the first published report of Wolbachia associated with H. humuli, expanding the known association of this endosymbiont to three genera of plant-parasitic nematodes. This finding will enhance sequence resources for further comparisons of Wolbachia diversity. [Figure: see text] The author(s) have dedicated the work to the public domain under the Creative Commons CC0 “No Rights Reserved” license by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law, 2024.https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-05-24-0054-Abacterial endosymbiontHeterodera humulilong-readsWolbachia |
| spellingShingle | Lester A. Núñez-Rodríguez Catherine L. Wram Cedar Hesse Inga A. Zasada Draft Genome Resource of a Wolbachia Endosymbiont in Heterodera humuli PhytoFrontiers bacterial endosymbiont Heterodera humuli long-reads Wolbachia |
| title | Draft Genome Resource of a Wolbachia Endosymbiont in Heterodera humuli |
| title_full | Draft Genome Resource of a Wolbachia Endosymbiont in Heterodera humuli |
| title_fullStr | Draft Genome Resource of a Wolbachia Endosymbiont in Heterodera humuli |
| title_full_unstemmed | Draft Genome Resource of a Wolbachia Endosymbiont in Heterodera humuli |
| title_short | Draft Genome Resource of a Wolbachia Endosymbiont in Heterodera humuli |
| title_sort | draft genome resource of a wolbachia endosymbiont in heterodera humuli |
| topic | bacterial endosymbiont Heterodera humuli long-reads Wolbachia |
| url | https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-05-24-0054-A |
| work_keys_str_mv | AT lesteranunezrodriguez draftgenomeresourceofawolbachiaendosymbiontinheteroderahumuli AT catherinelwram draftgenomeresourceofawolbachiaendosymbiontinheteroderahumuli AT cedarhesse draftgenomeresourceofawolbachiaendosymbiontinheteroderahumuli AT ingaazasada draftgenomeresourceofawolbachiaendosymbiontinheteroderahumuli |