Effect of the photothermal agent Cy7-TCF on oxidative stress and apoptosis in human pancreatic cancer cells
Objective To investigate the effect of the photothermal agent Cy7-TCF on oxidative stress and apoptosis in human pancreatic cancer cells. Methods Mia PaCa-2 cells were divided into control group, Cy7-TCF group (treated with Cy7-TCF alone), and Cy7-TCF+laser group (treated with Cy7-TCF followed by 80...
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| Format: | Article |
| Language: | zho |
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Editorial Office of Journal of Precision Medicine
2025-06-01
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| Series: | 精准医学杂志 |
| Subjects: | |
| Online Access: | https://jpmed.qdu.edu.cn/fileup/2096-529X/PDF/1750385688541-267236466.pdf |
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| Summary: | Objective To investigate the effect of the photothermal agent Cy7-TCF on oxidative stress and apoptosis in human pancreatic cancer cells. Methods Mia PaCa-2 cells were divided into control group, Cy7-TCF group (treated with Cy7-TCF alone), and Cy7-TCF+laser group (treated with Cy7-TCF followed by 808 nm laser irradiation). CCK-8 assay was used to measure cell viability under different concentrations of Cy7-TCF with or without laser irradiation; fluorescence microscopy was used to observe the uptake of Cy7-TCF by cells, and confocal microscopy combined with a lysosomal probe was used to observe the subcellular localization of Cy7-TCF; the DCFH-DA fluorescent probe was used to measure the level of reactive oxygen species (ROS) in cells; flow cytometry was used to measure cell apoptosis rate. Results CCK-8 assay showed that under laser irradiation, cell viability significantly decreased with the increasing concentration of Cy7-TCF in a concentration-dependent manner (P<0.05). Fluo-rescence microscopy showed that Cy7-TCF was effectively taken up by Mia PaCa-2 cells, and confocal microscopy showed that Cy7-TCF was mainly localized within lysosomes. DCFH-DA probe detection showed that under 808 nm laser irradiation, Cy7-TCF significantly increased the level of ROS in Mia PaCa-2 cells (q=19.07,16.84,P<0.05). Flow cytometry showed that the Cy7-TCF+laser group had a significantly higher apoptosis rate than the control group and the Cy7-TCF group (q=28.78,24.89,P<0.05). Conclusion Cy7-TCF is effectively taken up by Mia PaCa-2 cells and is localized in lysosomes, and through its photothermal effect, it induces the increase in the level of ROS in cells, reduces cell viability, and promotes cell apoptosis. |
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| ISSN: | 2096-529X |