Circannual Clock in <i>Laelia speciosa</i> (Orchidaceae) Through Dormancy vs. Germination Dynamics of Seeds Stored Under Controlled Conditions
This study aimed to determine the dynamics of dormancy using triphenyl tetrazolium chloride (TTC) and asymbiotic germination in <i>Laelia speciosa</i> (Kunth) Schltr. seeds stored for three years and one year at different temperatures. This is the first report of a circannual rhythm in &...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/14/3/336 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | This study aimed to determine the dynamics of dormancy using triphenyl tetrazolium chloride (TTC) and asymbiotic germination in <i>Laelia speciosa</i> (Kunth) Schltr. seeds stored for three years and one year at different temperatures. This is the first report of a circannual rhythm in <i>L. speciosa</i> seeds under controlled storage conditions. Two experiments were carried out: (a) with seeds from wild populations of <i>L. speciosa</i> collected at two different times and dehydrated to 4% relative humidity (RH) and stored for three years at 25 °C, 6 °C, −20 °C, and −80 °C, and (b) with seeds from six fruits stored in liquid nitrogen (LN2; −196 °C) at 25 °C for 12 months. The germination conditions were 25 ± 1 °C with 16 h of light (23 μmol m<sup>−2</sup> s<sup>−1</sup>) and 8 h of darkness for both trials. Because they have a rudimentary embryo, orchids are attributed a morphological latency; however, the staining of the embryo with TTC (>92%) in all the evaluations carried out throughout a year and the decrease in asymbiotic germination in the intermediate evaluations record a circannual biological cycle or clock, under temperature and humidity control (4% RH). |
|---|---|
| ISSN: | 2223-7747 |