Phytochemical Composition of Solanum retroflexum Analysed with the Aid of Ultra-Performance Liquid Chromatography Hyphenated to Quadrupole-Time-of-Flight Mass Spectrometry (UPLC-qTOF-MS)

Solanum retroflexum (nightshade) is an edible plant that is consumed in some regions of South Africa. Its leaves are a good source of vitamins, proteins, and minerals. It appears that there is no scientific report about the phytochemical composition of S. retroflexum. Here, ultra-performance liquid...

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Main Authors: Grace Daji, Paul Steenkamp, Ntakadzeni Madala, Bhekisisa Dlamini
Format: Article
Language:English
Published: Wiley 2018-01-01
Series:Journal of Food Quality
Online Access:http://dx.doi.org/10.1155/2018/3678795
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author Grace Daji
Paul Steenkamp
Ntakadzeni Madala
Bhekisisa Dlamini
author_facet Grace Daji
Paul Steenkamp
Ntakadzeni Madala
Bhekisisa Dlamini
author_sort Grace Daji
collection DOAJ
description Solanum retroflexum (nightshade) is an edible plant that is consumed in some regions of South Africa. Its leaves are a good source of vitamins, proteins, and minerals. It appears that there is no scientific report about the phytochemical composition of S. retroflexum. Here, ultra-performance liquid chromatography coupled to quadruple-time-of-flight mass spectrometry (UPLC-qTOF-MS) technique was use to achieve an untargeted metabolite fingerprinting of this plant. A total of 30 phytochemicals, including alkaloids, flavonoids, and cinnamic acids derivatives, were identified from the methanolic leaf extracts. The concentration of solvent did not affect the type of compounds extracted. The extraction resulted in qualitative yields of molecules such as quercetin-3-rutinoside, kaempferol-3-0-rutinoside, kaempferol-3-0-glucoside, 3-caffeoylquinic acid, 5-caffeoylquinic acid, and 3, 4-di-caffeoylquinic acid. The present study confirms the presence of phytochemical compounds in S. retroflexum similar to other Solanum plants.
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issn 0146-9428
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publishDate 2018-01-01
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record_format Article
series Journal of Food Quality
spelling doaj-art-5bb121f9c5e246d5a0ca72bf36af90312025-08-20T02:05:14ZengWileyJournal of Food Quality0146-94281745-45572018-01-01201810.1155/2018/36787953678795Phytochemical Composition of Solanum retroflexum Analysed with the Aid of Ultra-Performance Liquid Chromatography Hyphenated to Quadrupole-Time-of-Flight Mass Spectrometry (UPLC-qTOF-MS)Grace Daji0Paul Steenkamp1Ntakadzeni Madala2Bhekisisa Dlamini3Department of Biotechnology and Food Technology, Faculty of Science, University of Johannesburg, P.O. Box 17011, Doornfontein Campus, Gauteng 2028, South AfricaDepartment of Biochemistry, University of Johannesburg, P.O. Box 524, Auckland Park 2006, South AfricaDepartment of Biochemistry, University of Johannesburg, P.O. Box 524, Auckland Park 2006, South AfricaDepartment of Biotechnology and Food Technology, Faculty of Science, University of Johannesburg, P.O. Box 17011, Doornfontein Campus, Gauteng 2028, South AfricaSolanum retroflexum (nightshade) is an edible plant that is consumed in some regions of South Africa. Its leaves are a good source of vitamins, proteins, and minerals. It appears that there is no scientific report about the phytochemical composition of S. retroflexum. Here, ultra-performance liquid chromatography coupled to quadruple-time-of-flight mass spectrometry (UPLC-qTOF-MS) technique was use to achieve an untargeted metabolite fingerprinting of this plant. A total of 30 phytochemicals, including alkaloids, flavonoids, and cinnamic acids derivatives, were identified from the methanolic leaf extracts. The concentration of solvent did not affect the type of compounds extracted. The extraction resulted in qualitative yields of molecules such as quercetin-3-rutinoside, kaempferol-3-0-rutinoside, kaempferol-3-0-glucoside, 3-caffeoylquinic acid, 5-caffeoylquinic acid, and 3, 4-di-caffeoylquinic acid. The present study confirms the presence of phytochemical compounds in S. retroflexum similar to other Solanum plants.http://dx.doi.org/10.1155/2018/3678795
spellingShingle Grace Daji
Paul Steenkamp
Ntakadzeni Madala
Bhekisisa Dlamini
Phytochemical Composition of Solanum retroflexum Analysed with the Aid of Ultra-Performance Liquid Chromatography Hyphenated to Quadrupole-Time-of-Flight Mass Spectrometry (UPLC-qTOF-MS)
Journal of Food Quality
title Phytochemical Composition of Solanum retroflexum Analysed with the Aid of Ultra-Performance Liquid Chromatography Hyphenated to Quadrupole-Time-of-Flight Mass Spectrometry (UPLC-qTOF-MS)
title_full Phytochemical Composition of Solanum retroflexum Analysed with the Aid of Ultra-Performance Liquid Chromatography Hyphenated to Quadrupole-Time-of-Flight Mass Spectrometry (UPLC-qTOF-MS)
title_fullStr Phytochemical Composition of Solanum retroflexum Analysed with the Aid of Ultra-Performance Liquid Chromatography Hyphenated to Quadrupole-Time-of-Flight Mass Spectrometry (UPLC-qTOF-MS)
title_full_unstemmed Phytochemical Composition of Solanum retroflexum Analysed with the Aid of Ultra-Performance Liquid Chromatography Hyphenated to Quadrupole-Time-of-Flight Mass Spectrometry (UPLC-qTOF-MS)
title_short Phytochemical Composition of Solanum retroflexum Analysed with the Aid of Ultra-Performance Liquid Chromatography Hyphenated to Quadrupole-Time-of-Flight Mass Spectrometry (UPLC-qTOF-MS)
title_sort phytochemical composition of solanum retroflexum analysed with the aid of ultra performance liquid chromatography hyphenated to quadrupole time of flight mass spectrometry uplc qtof ms
url http://dx.doi.org/10.1155/2018/3678795
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