Evaluation of magnetic carriers employed for immobilization of lipase from candida rugosa
Currently, the use of magnetic nanoparticles has aroused interest in industrial processes, and the combination of their properties with the immobilization of lipases has been developed in order to produce carriers of easy separation of the reaction medium. In this context, the objective of the pres...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Viçosa (UFV)
2020-10-01
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| Series: | The Journal of Engineering and Exact Sciences |
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| Online Access: | https://periodicos.ufv.br/jcec/article/view/10858 |
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| author | Otávio Domingues Letícia Karen dos Santos Rondinelli Donizetti Herculano Danilo Luiz Flumignan Ariela Veloso de Paula |
| author_facet | Otávio Domingues Letícia Karen dos Santos Rondinelli Donizetti Herculano Danilo Luiz Flumignan Ariela Veloso de Paula |
| author_sort | Otávio Domingues |
| collection | DOAJ |
| description |
Currently, the use of magnetic nanoparticles has aroused interest in industrial processes, and the combination of their properties with the immobilization of lipases has been developed in order to produce carriers of easy separation of the reaction medium. In this context, the objective of the present study was to immobilize Candida rugosa lipase in magnetic nanoparticles, such as magnetite and maghemite, by physical adsorption and covalent bonding. The biocatalysts were evaluated by infrared spectroscopy (FTIR) and hydrolytic activity analysis. Thus, from the analyses performed, the best biocatalyst obtained was the immobilized by covalent bond in maghemite, presenting a hydrolytic activity of 174.67 U/g.
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| format | Article |
| id | doaj-art-5baebcff78f94ea2acb5c51591a0ef33 |
| institution | Kabale University |
| issn | 2527-1075 |
| language | English |
| publishDate | 2020-10-01 |
| publisher | Universidade Federal de Viçosa (UFV) |
| record_format | Article |
| series | The Journal of Engineering and Exact Sciences |
| spelling | doaj-art-5baebcff78f94ea2acb5c51591a0ef332025-02-02T19:57:58ZengUniversidade Federal de Viçosa (UFV)The Journal of Engineering and Exact Sciences2527-10752020-10-016410.18540/jcecvl6iss4pp0498-0504Evaluation of magnetic carriers employed for immobilization of lipase from candida rugosa Otávio Domingues0Letícia Karen dos Santos1Rondinelli Donizetti Herculano2Danilo Luiz Flumignan3Ariela Veloso de Paula4Universidade Estadual Paulista "Júlio de Mesquita Filho"Universidade Estadual Paulista "Júlio de Mesquita Filho"Universidade Estadual Paulista "Júlio de Mesquita Filho"Universidade Estadual Paulista "Júlio de Mesquita Filho"Universidade Estadual Paulista "Júlio de Mesquita Filho" Currently, the use of magnetic nanoparticles has aroused interest in industrial processes, and the combination of their properties with the immobilization of lipases has been developed in order to produce carriers of easy separation of the reaction medium. In this context, the objective of the present study was to immobilize Candida rugosa lipase in magnetic nanoparticles, such as magnetite and maghemite, by physical adsorption and covalent bonding. The biocatalysts were evaluated by infrared spectroscopy (FTIR) and hydrolytic activity analysis. Thus, from the analyses performed, the best biocatalyst obtained was the immobilized by covalent bond in maghemite, presenting a hydrolytic activity of 174.67 U/g. https://periodicos.ufv.br/jcec/article/view/10858Infrared spectroscopyEnzyme immobilizationCovalent bondingMaghemiteMagnetic nanoparticles |
| spellingShingle | Otávio Domingues Letícia Karen dos Santos Rondinelli Donizetti Herculano Danilo Luiz Flumignan Ariela Veloso de Paula Evaluation of magnetic carriers employed for immobilization of lipase from candida rugosa The Journal of Engineering and Exact Sciences Infrared spectroscopy Enzyme immobilization Covalent bonding Maghemite Magnetic nanoparticles |
| title | Evaluation of magnetic carriers employed for immobilization of lipase from candida rugosa |
| title_full | Evaluation of magnetic carriers employed for immobilization of lipase from candida rugosa |
| title_fullStr | Evaluation of magnetic carriers employed for immobilization of lipase from candida rugosa |
| title_full_unstemmed | Evaluation of magnetic carriers employed for immobilization of lipase from candida rugosa |
| title_short | Evaluation of magnetic carriers employed for immobilization of lipase from candida rugosa |
| title_sort | evaluation of magnetic carriers employed for immobilization of lipase from candida rugosa |
| topic | Infrared spectroscopy Enzyme immobilization Covalent bonding Maghemite Magnetic nanoparticles |
| url | https://periodicos.ufv.br/jcec/article/view/10858 |
| work_keys_str_mv | AT otaviodomingues evaluationofmagneticcarriersemployedforimmobilizationoflipasefromcandidarugosa AT leticiakarendossantos evaluationofmagneticcarriersemployedforimmobilizationoflipasefromcandidarugosa AT rondinellidonizettiherculano evaluationofmagneticcarriersemployedforimmobilizationoflipasefromcandidarugosa AT daniloluizflumignan evaluationofmagneticcarriersemployedforimmobilizationoflipasefromcandidarugosa AT arielavelosodepaula evaluationofmagneticcarriersemployedforimmobilizationoflipasefromcandidarugosa |