Warifteine, an Alkaloid Purified from Cissampelos sympodialis, Inhibits Neutrophil Migration In Vitro and In Vivo
Cissampelos sympodialis Eichl is a plant from the Northeast and Southeast of Brazil. Its root infusion is popularly used for treatment of inflammatory and allergic diseases. We investigated whether warifteine, its main alkaloid, would have anti-inflammatory effect due to a blockage of neutrophil fun...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2014/752923 |
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| author | Thaline F. A. Lima Juliana D. B. Rocha Anderson B. Guimarães-Costa José M. Barbosa-Filho Débora Decoté-Ricardo Elvira M. Saraiva Luciana B. Arruda Marcia R. Piuvezam Ligia M. T. Peçanha |
| author_facet | Thaline F. A. Lima Juliana D. B. Rocha Anderson B. Guimarães-Costa José M. Barbosa-Filho Débora Decoté-Ricardo Elvira M. Saraiva Luciana B. Arruda Marcia R. Piuvezam Ligia M. T. Peçanha |
| author_sort | Thaline F. A. Lima |
| collection | DOAJ |
| description | Cissampelos sympodialis Eichl is a plant from the Northeast and Southeast of Brazil. Its root infusion is popularly used for treatment of inflammatory and allergic diseases. We investigated whether warifteine, its main alkaloid, would have anti-inflammatory effect due to a blockage of neutrophil function. In vivo warifteine treatment inhibited casein-induced neutrophil migration to the peritoneal cavity but did not inhibit neutrophil mobilization from the bone marrow. Analysis of the direct effect of warifteine upon neutrophil adherence and migration in vitro demonstrated that the alkaloid decreased cell adhesion to P and E-selectin-transfected cells. In addition, fLMP-induced neutrophil migration in a transwell system was blocked by warifteine; this effect was mimicked by cAMP mimetic/inducing substances, and warifteine increased intracellular cAMP levels in neutrophils. The production of DNA extracellular traps (NETs) was also blocked by warifteine but there was no alteration on PMA-induced oxidative burst or LPS-stimulated TNFα secretion. Taken together, our data indicate that the alkaloid warifteine is a potent anti-inflammatory substance and that it has an effect on neutrophil migration through a decrease in both cell adhesion and migration. |
| format | Article |
| id | doaj-art-5b970a0b429d4875ac8380c3a6821fa5 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-5b970a0b429d4875ac8380c3a6821fa52025-02-03T06:01:19ZengWileyJournal of Immunology Research2314-88612314-71562014-01-01201410.1155/2014/752923752923Warifteine, an Alkaloid Purified from Cissampelos sympodialis, Inhibits Neutrophil Migration In Vitro and In VivoThaline F. A. Lima0Juliana D. B. Rocha1Anderson B. Guimarães-Costa2José M. Barbosa-Filho3Débora Decoté-Ricardo4Elvira M. Saraiva5Luciana B. Arruda6Marcia R. Piuvezam7Ligia M. T. Peçanha8Departamento de Imunologia, Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, CCS, Bloco I, Sala I2-062, Ilha do Fundão, 21944-570 Rio de Janeiro, RJ, BrazilDepartamento de Imunologia, Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, CCS, Bloco I, Sala I2-062, Ilha do Fundão, 21944-570 Rio de Janeiro, RJ, BrazilDepartamento de Imunologia, Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, CCS, Bloco I, Sala I2-062, Ilha do Fundão, 21944-570 Rio de Janeiro, RJ, BrazilDepartamento de Fisiologia e Patologia, Universidade Federal da Paraíba, 58051-970 João Pessoa, PB, BrazilInstituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, 23890-000 Seropédica, RJ, BrazilDepartamento de Imunologia, Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, CCS, Bloco I, Sala I2-062, Ilha do Fundão, 21944-570 Rio de Janeiro, RJ, BrazilDepartamento de Virologia, Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, 21944-570 Rio de Janeiro, RJ, BrazilDepartamento de Fisiologia e Patologia, Universidade Federal da Paraíba, 58051-970 João Pessoa, PB, BrazilDepartamento de Imunologia, Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, CCS, Bloco I, Sala I2-062, Ilha do Fundão, 21944-570 Rio de Janeiro, RJ, BrazilCissampelos sympodialis Eichl is a plant from the Northeast and Southeast of Brazil. Its root infusion is popularly used for treatment of inflammatory and allergic diseases. We investigated whether warifteine, its main alkaloid, would have anti-inflammatory effect due to a blockage of neutrophil function. In vivo warifteine treatment inhibited casein-induced neutrophil migration to the peritoneal cavity but did not inhibit neutrophil mobilization from the bone marrow. Analysis of the direct effect of warifteine upon neutrophil adherence and migration in vitro demonstrated that the alkaloid decreased cell adhesion to P and E-selectin-transfected cells. In addition, fLMP-induced neutrophil migration in a transwell system was blocked by warifteine; this effect was mimicked by cAMP mimetic/inducing substances, and warifteine increased intracellular cAMP levels in neutrophils. The production of DNA extracellular traps (NETs) was also blocked by warifteine but there was no alteration on PMA-induced oxidative burst or LPS-stimulated TNFα secretion. Taken together, our data indicate that the alkaloid warifteine is a potent anti-inflammatory substance and that it has an effect on neutrophil migration through a decrease in both cell adhesion and migration.http://dx.doi.org/10.1155/2014/752923 |
| spellingShingle | Thaline F. A. Lima Juliana D. B. Rocha Anderson B. Guimarães-Costa José M. Barbosa-Filho Débora Decoté-Ricardo Elvira M. Saraiva Luciana B. Arruda Marcia R. Piuvezam Ligia M. T. Peçanha Warifteine, an Alkaloid Purified from Cissampelos sympodialis, Inhibits Neutrophil Migration In Vitro and In Vivo Journal of Immunology Research |
| title | Warifteine, an Alkaloid Purified from Cissampelos sympodialis, Inhibits Neutrophil Migration In Vitro and In Vivo |
| title_full | Warifteine, an Alkaloid Purified from Cissampelos sympodialis, Inhibits Neutrophil Migration In Vitro and In Vivo |
| title_fullStr | Warifteine, an Alkaloid Purified from Cissampelos sympodialis, Inhibits Neutrophil Migration In Vitro and In Vivo |
| title_full_unstemmed | Warifteine, an Alkaloid Purified from Cissampelos sympodialis, Inhibits Neutrophil Migration In Vitro and In Vivo |
| title_short | Warifteine, an Alkaloid Purified from Cissampelos sympodialis, Inhibits Neutrophil Migration In Vitro and In Vivo |
| title_sort | warifteine an alkaloid purified from cissampelos sympodialis inhibits neutrophil migration in vitro and in vivo |
| url | http://dx.doi.org/10.1155/2014/752923 |
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