Identification of FANCG as a prognostic factor for prostate cancer
Abstract Background To ascertain role of the fanconi anemia complementation group G (FANCG) gene in prostate cancer (PCa) and the suitability of the FANCG gene as a prognostic biomarker for PCa. Methods We assessed the expression of FANCG using an analysis of publicly available data sets and cell li...
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| Language: | English |
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BMC
2025-07-01
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| Series: | European Journal of Medical Research |
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| Online Access: | https://doi.org/10.1186/s40001-025-02877-w |
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| author | Wenlong Gao Peng Liu Han Xinyue Yuanjie Niu Wu Yi |
| author_facet | Wenlong Gao Peng Liu Han Xinyue Yuanjie Niu Wu Yi |
| author_sort | Wenlong Gao |
| collection | DOAJ |
| description | Abstract Background To ascertain role of the fanconi anemia complementation group G (FANCG) gene in prostate cancer (PCa) and the suitability of the FANCG gene as a prognostic biomarker for PCa. Methods We assessed the expression of FANCG using an analysis of publicly available data sets and cell lines. Physiological functioning of the cells was evaluated through MTT assays and migration assays. Co-expressed genes and enrichment analysis were conducted to probe the biological significance of FANCG in PCa. Quantitative real-time polymerase chain reaction (qPCR) was utilized to detect the expression levels of hub genes (MCM7, MCM5, POLD1, POLA2, LIG1) associated with FANCG. Results We observed a significant upregulation of FANCG expression in PCa patients and cell lines. Furthermore, immunohistochemical analysis demonstrated markedly higher FANCG protein expression in PCa tissues compared to non-cancerous PCa tissues. Downregulation of FANCG significantly inhibited cell proliferation and migration potential. Evaluation of FANCG-related hub genes (MCM7, MCM5, POLD1, POLA2, and LIG1) revealed their close association with cell cycle-related signaling pathways. Upregulation of FANCG mRNA expression in PCa tissues significantly correlated with high serum PSA levels, advanced pathological stage, high Gleason score, shorter overall survival time, and shorter disease-free survival time. Conclusions This study suggests that FANCG likely plays a pivotal role in PCa progression. In addition, increased FANCG expression may serve as an indicator of poor disease-free survival and an adverse prognosis for PCa patients. |
| format | Article |
| id | doaj-art-5b90f5bb42654751af2a64d412df6053 |
| institution | DOAJ |
| issn | 2047-783X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | European Journal of Medical Research |
| spelling | doaj-art-5b90f5bb42654751af2a64d412df60532025-08-20T03:04:31ZengBMCEuropean Journal of Medical Research2047-783X2025-07-0130111110.1186/s40001-025-02877-wIdentification of FANCG as a prognostic factor for prostate cancerWenlong Gao0Peng Liu1Han Xinyue2Yuanjie Niu3Wu Yi4Department of Urology, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, University of Electronic Science and Technology of ChinaNankai UniversityDepartment of Urology, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, University of Electronic Science and Technology of ChinaNankai UniversityDepartment of Urology, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, University of Electronic Science and Technology of ChinaAbstract Background To ascertain role of the fanconi anemia complementation group G (FANCG) gene in prostate cancer (PCa) and the suitability of the FANCG gene as a prognostic biomarker for PCa. Methods We assessed the expression of FANCG using an analysis of publicly available data sets and cell lines. Physiological functioning of the cells was evaluated through MTT assays and migration assays. Co-expressed genes and enrichment analysis were conducted to probe the biological significance of FANCG in PCa. Quantitative real-time polymerase chain reaction (qPCR) was utilized to detect the expression levels of hub genes (MCM7, MCM5, POLD1, POLA2, LIG1) associated with FANCG. Results We observed a significant upregulation of FANCG expression in PCa patients and cell lines. Furthermore, immunohistochemical analysis demonstrated markedly higher FANCG protein expression in PCa tissues compared to non-cancerous PCa tissues. Downregulation of FANCG significantly inhibited cell proliferation and migration potential. Evaluation of FANCG-related hub genes (MCM7, MCM5, POLD1, POLA2, and LIG1) revealed their close association with cell cycle-related signaling pathways. Upregulation of FANCG mRNA expression in PCa tissues significantly correlated with high serum PSA levels, advanced pathological stage, high Gleason score, shorter overall survival time, and shorter disease-free survival time. Conclusions This study suggests that FANCG likely plays a pivotal role in PCa progression. In addition, increased FANCG expression may serve as an indicator of poor disease-free survival and an adverse prognosis for PCa patients.https://doi.org/10.1186/s40001-025-02877-wProstate cancerFanconi anemia complementation group GPrognostic factorBioinformatics |
| spellingShingle | Wenlong Gao Peng Liu Han Xinyue Yuanjie Niu Wu Yi Identification of FANCG as a prognostic factor for prostate cancer European Journal of Medical Research Prostate cancer Fanconi anemia complementation group G Prognostic factor Bioinformatics |
| title | Identification of FANCG as a prognostic factor for prostate cancer |
| title_full | Identification of FANCG as a prognostic factor for prostate cancer |
| title_fullStr | Identification of FANCG as a prognostic factor for prostate cancer |
| title_full_unstemmed | Identification of FANCG as a prognostic factor for prostate cancer |
| title_short | Identification of FANCG as a prognostic factor for prostate cancer |
| title_sort | identification of fancg as a prognostic factor for prostate cancer |
| topic | Prostate cancer Fanconi anemia complementation group G Prognostic factor Bioinformatics |
| url | https://doi.org/10.1186/s40001-025-02877-w |
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