Degradation rate and weight loss analysis for freeze-dried, decellularized, and deproteinized bovine bone scaffolds
Background: Mandibular defects, often caused by trauma, tumors, infections, and congenital issues, are commonly treated with bone grafts. Tissue engineering plays a crucial role in bone reconstruction, with scaffolds such as deproteinized bovine bone matrix (DBBM), freeze-dried bovine bone (FDBB), a...
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| Format: | Article |
| Language: | English |
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Universitas Airlangga
2025-03-01
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| Series: | Dental Journal |
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| Online Access: | https://e-journal.unair.ac.id/MKG/article/view/53028 |
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| author | Ramadhan Pramudya Vidarta David Buntoro Kamadjaja Coen Pramono Danudiningrat Muhammad Subhan Amir Andra Rizqiawan Anita Yuliati Darmawan Darwis |
| author_facet | Ramadhan Pramudya Vidarta David Buntoro Kamadjaja Coen Pramono Danudiningrat Muhammad Subhan Amir Andra Rizqiawan Anita Yuliati Darmawan Darwis |
| author_sort | Ramadhan Pramudya Vidarta |
| collection | DOAJ |
| description | Background: Mandibular defects, often caused by trauma, tumors, infections, and congenital issues, are commonly treated with bone grafts. Tissue engineering plays a crucial role in bone reconstruction, with scaffolds such as deproteinized bovine bone matrix (DBBM), freeze-dried bovine bone (FDBB), and decellularized FDBB (Dc-FDBB) being studied for their efficacy. Decellularization reduces the antigenic potential of FDBB. These scaffolds are designed to degrade within the body. Purpose: To analyze the weight loss and degradation rates of FDBB and Dc-FDBB materials, using DBBM as a control. Methods: This in vitro experimental study, conducted over 2 months, employed a cross-sectional approach to analyze the weight loss and degradation rates of FDBB, Dc-FDBB, and DBBM scaffolds in a simulated body fluid (SBF) solution. Results: Under dynamic immersion conditions, DBBM exhibited the highest daily weight loss at 0.741% and a degradation rate of 0.466 mg/cm²/day, followed by Dc-FDBB at 0.568% and 0.418 mg/cm²/day and FDBB at 0.525% and 0.385 mg/cm²/day. Under static immersion conditions, DBBM also showed the highest weight loss at 0.255%, with a degradation rate of 0.165 mg/cm²/day, followed by Dc-FDBB at 0.245% and 0.163 mg/cm²/day, and FDBB at 0.168% with a degradation rate of 0.126 mg/cm²/day. Significant differences were observed between scaffold groups (p = 0.000). Conclusion: DBBM, Dc-FDBB, and FDBB scaffolds meet the optimal requirements for tissue engineering materials based on their weight loss and degradation rates. DBBM demonstrated the highest values among the scaffolds analyzed. |
| format | Article |
| id | doaj-art-5b8a50b20dbf4e019fd9fbde4126a7c6 |
| institution | OA Journals |
| issn | 1978-3728 2442-9740 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Dental Journal |
| spelling | doaj-art-5b8a50b20dbf4e019fd9fbde4126a7c62025-08-20T02:32:25ZengUniversitas AirlanggaDental Journal1978-37282442-97402025-03-01581232910.20473/j.djmkg.v58.i1.p23-2951189Degradation rate and weight loss analysis for freeze-dried, decellularized, and deproteinized bovine bone scaffoldsRamadhan Pramudya Vidarta0https://orcid.org/0009-0001-7911-139XDavid Buntoro Kamadjaja1https://orcid.org/0000-0003-0162-5451Coen Pramono Danudiningrat2https://orcid.org/0009-0003-9022-8884Muhammad Subhan Amir3https://orcid.org/0000-0002-0522-9758Andra Rizqiawan4https://orcid.org/0000-0001-5306-7338Anita Yuliati5Darmawan Darwis6https://orcid.org/0000-0002-5091-0526Magister Program of Clinical Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia; Resident of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, IndonesiaDepartment of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, IndonesiaDepartment of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, IndonesiaDepartment of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, IndonesiaDepartment of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, IndonesiaDepartment of Dentistry Materials Science, Faculty of Dental Medicine Universitas Airlangga, Surabaya, IndonesiaResearch Center for Radiation Process Technology Research Organization for Nuclear Energy, National Research and Innovation Agency, IndonesiaBackground: Mandibular defects, often caused by trauma, tumors, infections, and congenital issues, are commonly treated with bone grafts. Tissue engineering plays a crucial role in bone reconstruction, with scaffolds such as deproteinized bovine bone matrix (DBBM), freeze-dried bovine bone (FDBB), and decellularized FDBB (Dc-FDBB) being studied for their efficacy. Decellularization reduces the antigenic potential of FDBB. These scaffolds are designed to degrade within the body. Purpose: To analyze the weight loss and degradation rates of FDBB and Dc-FDBB materials, using DBBM as a control. Methods: This in vitro experimental study, conducted over 2 months, employed a cross-sectional approach to analyze the weight loss and degradation rates of FDBB, Dc-FDBB, and DBBM scaffolds in a simulated body fluid (SBF) solution. Results: Under dynamic immersion conditions, DBBM exhibited the highest daily weight loss at 0.741% and a degradation rate of 0.466 mg/cm²/day, followed by Dc-FDBB at 0.568% and 0.418 mg/cm²/day and FDBB at 0.525% and 0.385 mg/cm²/day. Under static immersion conditions, DBBM also showed the highest weight loss at 0.255%, with a degradation rate of 0.165 mg/cm²/day, followed by Dc-FDBB at 0.245% and 0.163 mg/cm²/day, and FDBB at 0.168% with a degradation rate of 0.126 mg/cm²/day. Significant differences were observed between scaffold groups (p = 0.000). Conclusion: DBBM, Dc-FDBB, and FDBB scaffolds meet the optimal requirements for tissue engineering materials based on their weight loss and degradation rates. DBBM demonstrated the highest values among the scaffolds analyzed.https://e-journal.unair.ac.id/MKG/article/view/53028biomaterialdbbmdc-fdbbfdbbmaxillofacial defectscaffoldtissue engineering |
| spellingShingle | Ramadhan Pramudya Vidarta David Buntoro Kamadjaja Coen Pramono Danudiningrat Muhammad Subhan Amir Andra Rizqiawan Anita Yuliati Darmawan Darwis Degradation rate and weight loss analysis for freeze-dried, decellularized, and deproteinized bovine bone scaffolds Dental Journal biomaterial dbbm dc-fdbb fdbb maxillofacial defect scaffold tissue engineering |
| title | Degradation rate and weight loss analysis for freeze-dried, decellularized, and deproteinized bovine bone scaffolds |
| title_full | Degradation rate and weight loss analysis for freeze-dried, decellularized, and deproteinized bovine bone scaffolds |
| title_fullStr | Degradation rate and weight loss analysis for freeze-dried, decellularized, and deproteinized bovine bone scaffolds |
| title_full_unstemmed | Degradation rate and weight loss analysis for freeze-dried, decellularized, and deproteinized bovine bone scaffolds |
| title_short | Degradation rate and weight loss analysis for freeze-dried, decellularized, and deproteinized bovine bone scaffolds |
| title_sort | degradation rate and weight loss analysis for freeze dried decellularized and deproteinized bovine bone scaffolds |
| topic | biomaterial dbbm dc-fdbb fdbb maxillofacial defect scaffold tissue engineering |
| url | https://e-journal.unair.ac.id/MKG/article/view/53028 |
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