Persistent salmonellosis causes pancreatitis in a murine model of infection.
Pancreatitis, a known risk factor for the development of pancreatic ductal adenocarcinoma, is a serious, widespread medical condition usually caused by alcohol abuse or gallstone-mediated ductal obstruction. However, many cases of pancreatitis are of an unknown etiology. Pancreatitis has been linked...
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0092807&type=printable |
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| author | Kathleen E DelGiorno Jason W Tam Jason C Hall Gangadaar Thotakura Howard C Crawford Adrianus W M van der Velden |
| author_facet | Kathleen E DelGiorno Jason W Tam Jason C Hall Gangadaar Thotakura Howard C Crawford Adrianus W M van der Velden |
| author_sort | Kathleen E DelGiorno |
| collection | DOAJ |
| description | Pancreatitis, a known risk factor for the development of pancreatic ductal adenocarcinoma, is a serious, widespread medical condition usually caused by alcohol abuse or gallstone-mediated ductal obstruction. However, many cases of pancreatitis are of an unknown etiology. Pancreatitis has been linked to bacterial infection, but causality has yet to be established. Here, we found that persistent infection of mice with the bacterial pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium) was sufficient to induce pancreatitis reminiscent of the human disease. Specifically, we found that pancreatitis induced by persistent S. Typhimurium infection was characterized by a loss of pancreatic acinar cells, acinar-to-ductal metaplasia, fibrosis and accumulation of inflammatory cells, including CD11b+ F4/80+, CD11b+ Ly6Cint Ly6G+ and CD11b+ Ly6Chi Ly6G- cells. Furthermore, we found that S. Typhimurium colonized and persisted in the pancreas, associated with pancreatic acinar cells in vivo, and could invade cultured pancreatic acinar cells in vitro. Thus, persistent infection of mice with S. Typhimurium may serve as a useful model for the study of pancreatitis as it relates to bacterial infection. Increased knowledge of how pathogenic bacteria can cause pancreatitis will provide a more integrated picture of the etiology of the disease and could lead to the development of new therapeutic approaches for treatment and prevention of pancreatitis and pancreatic ductal adenocarcinoma. |
| format | Article |
| id | doaj-art-5b70faa214d64b718cdbce884435c459 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-5b70faa214d64b718cdbce884435c4592025-08-20T02:22:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0194e9280710.1371/journal.pone.0092807Persistent salmonellosis causes pancreatitis in a murine model of infection.Kathleen E DelGiornoJason W TamJason C HallGangadaar ThotakuraHoward C CrawfordAdrianus W M van der VeldenPancreatitis, a known risk factor for the development of pancreatic ductal adenocarcinoma, is a serious, widespread medical condition usually caused by alcohol abuse or gallstone-mediated ductal obstruction. However, many cases of pancreatitis are of an unknown etiology. Pancreatitis has been linked to bacterial infection, but causality has yet to be established. Here, we found that persistent infection of mice with the bacterial pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium) was sufficient to induce pancreatitis reminiscent of the human disease. Specifically, we found that pancreatitis induced by persistent S. Typhimurium infection was characterized by a loss of pancreatic acinar cells, acinar-to-ductal metaplasia, fibrosis and accumulation of inflammatory cells, including CD11b+ F4/80+, CD11b+ Ly6Cint Ly6G+ and CD11b+ Ly6Chi Ly6G- cells. Furthermore, we found that S. Typhimurium colonized and persisted in the pancreas, associated with pancreatic acinar cells in vivo, and could invade cultured pancreatic acinar cells in vitro. Thus, persistent infection of mice with S. Typhimurium may serve as a useful model for the study of pancreatitis as it relates to bacterial infection. Increased knowledge of how pathogenic bacteria can cause pancreatitis will provide a more integrated picture of the etiology of the disease and could lead to the development of new therapeutic approaches for treatment and prevention of pancreatitis and pancreatic ductal adenocarcinoma.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0092807&type=printable |
| spellingShingle | Kathleen E DelGiorno Jason W Tam Jason C Hall Gangadaar Thotakura Howard C Crawford Adrianus W M van der Velden Persistent salmonellosis causes pancreatitis in a murine model of infection. PLoS ONE |
| title | Persistent salmonellosis causes pancreatitis in a murine model of infection. |
| title_full | Persistent salmonellosis causes pancreatitis in a murine model of infection. |
| title_fullStr | Persistent salmonellosis causes pancreatitis in a murine model of infection. |
| title_full_unstemmed | Persistent salmonellosis causes pancreatitis in a murine model of infection. |
| title_short | Persistent salmonellosis causes pancreatitis in a murine model of infection. |
| title_sort | persistent salmonellosis causes pancreatitis in a murine model of infection |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0092807&type=printable |
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