Whole‐Tumor Clearing and Imaging of Intratumor Microbiota in Three Dimensions with miCDaL Strategy

Whole‐Tumor Clearing and Imaging of Intratumor Microbiota in Three Dimensions with miCDaL Strategy

Abstract Acquiring detailed spatial information about intratumor microbiota in situ is challenging, which leaves 3D distributions of microbiota within entire tumors largely unexplored. Here, a modified iDISCO‐CUBIC tissue clearing and D‐amino acid microbiome labeling‐based (miCDaL) strategy are prop...

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Bibliographic Details
Main Authors: Yuezhou Wang, Zile Jiang, Kai Zhang, Huimin Tang, Guimei Wang, Jinshan Gao, Guanghui He, Baoyue Liang, Li Li, Chaoyong Yang, Xianming Deng
Format: Article
Language:English
Published: Wiley 2024-11-01
Series:Advanced Science
Subjects:
3D imaging
centimeter‐scale imaging depth
fluorescent D‐amino acid‐based probes
intratumor microbiota
tissue clearing
Online Access:https://doi.org/10.1002/advs.202400694
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