Isolation and Molecular Characterization of Peste des Petits Ruminants Virus from Outbreaks in Southern Ethiopia, 2020
Peste des petits ruminants (PPR) is one of the most important transboundary diseases of small ruminants. In this study, nasal and oral swabs (n = 24) were collected from sheep (n = 7) and goats (n = 17) with clinical signs in southern Ethiopia in March 2020. PPR virus was isolated on Vero dog cells...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Advances in Virology |
| Online Access: | http://dx.doi.org/10.1155/2022/5329898 |
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| author | Abde Aliy Mohammed Tesfaye Rufael Chibssa Waktole Terfa Fasil Aklilu Delesa Damena Redeat Belayneh Menbere Kidane |
| author_facet | Abde Aliy Mohammed Tesfaye Rufael Chibssa Waktole Terfa Fasil Aklilu Delesa Damena Redeat Belayneh Menbere Kidane |
| author_sort | Abde Aliy Mohammed |
| collection | DOAJ |
| description | Peste des petits ruminants (PPR) is one of the most important transboundary diseases of small ruminants. In this study, nasal and oral swabs (n = 24) were collected from sheep (n = 7) and goats (n = 17) with clinical signs in southern Ethiopia in March 2020. PPR virus was isolated on Vero dog cells expressing the signaling lymphocyte activation molecule (VDS) and screened using RT-qPCR. Positive samples were confirmed by conventional RT-PCR followed by sequencing of a partial nucleoprotein (N) gene segment. Results revealed that 54% (n = 13/24) of the tested samples were PPRV-positive Phylogenetic analysis revealed that the viruses belonged to lineage IV and lineage II. The lineage IV viruses were similar, although not identical, to other lineage IV viruses previously reported in Ethiopia and other East African countries while the lineage II viruses have been reported for the first time in Ethiopia showed a high nucleotide identity (99.06%) with the vaccine (Nigeria 75/1) that is currently used in Ethiopia for the prevention of PPR. Further investigations are therefore recommended in order to fully understand the true nature of the lineage II PPRVs in Ethiopia. |
| format | Article |
| id | doaj-art-5b6435d040d54634bb6ec58a73f7629c |
| institution | OA Journals |
| issn | 1687-8647 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Virology |
| spelling | doaj-art-5b6435d040d54634bb6ec58a73f7629c2025-08-20T02:05:13ZengWileyAdvances in Virology1687-86472022-01-01202210.1155/2022/5329898Isolation and Molecular Characterization of Peste des Petits Ruminants Virus from Outbreaks in Southern Ethiopia, 2020Abde Aliy Mohammed0Tesfaye Rufael Chibssa1Waktole Terfa2Fasil Aklilu3Delesa Damena4Redeat Belayneh5Menbere Kidane6National Animal Health Diagnostic and Investigation Center (NAHDIC)National Animal Health Diagnostic and Investigation Center (NAHDIC)Ambo UniversityNational Animal Health Diagnostic and Investigation Center (NAHDIC)National Animal Health Diagnostic and Investigation Center (NAHDIC)National Animal Health Diagnostic and Investigation Center (NAHDIC)National Animal Health Diagnostic and Investigation Center (NAHDIC)Peste des petits ruminants (PPR) is one of the most important transboundary diseases of small ruminants. In this study, nasal and oral swabs (n = 24) were collected from sheep (n = 7) and goats (n = 17) with clinical signs in southern Ethiopia in March 2020. PPR virus was isolated on Vero dog cells expressing the signaling lymphocyte activation molecule (VDS) and screened using RT-qPCR. Positive samples were confirmed by conventional RT-PCR followed by sequencing of a partial nucleoprotein (N) gene segment. Results revealed that 54% (n = 13/24) of the tested samples were PPRV-positive Phylogenetic analysis revealed that the viruses belonged to lineage IV and lineage II. The lineage IV viruses were similar, although not identical, to other lineage IV viruses previously reported in Ethiopia and other East African countries while the lineage II viruses have been reported for the first time in Ethiopia showed a high nucleotide identity (99.06%) with the vaccine (Nigeria 75/1) that is currently used in Ethiopia for the prevention of PPR. Further investigations are therefore recommended in order to fully understand the true nature of the lineage II PPRVs in Ethiopia.http://dx.doi.org/10.1155/2022/5329898 |
| spellingShingle | Abde Aliy Mohammed Tesfaye Rufael Chibssa Waktole Terfa Fasil Aklilu Delesa Damena Redeat Belayneh Menbere Kidane Isolation and Molecular Characterization of Peste des Petits Ruminants Virus from Outbreaks in Southern Ethiopia, 2020 Advances in Virology |
| title | Isolation and Molecular Characterization of Peste des Petits Ruminants Virus from Outbreaks in Southern Ethiopia, 2020 |
| title_full | Isolation and Molecular Characterization of Peste des Petits Ruminants Virus from Outbreaks in Southern Ethiopia, 2020 |
| title_fullStr | Isolation and Molecular Characterization of Peste des Petits Ruminants Virus from Outbreaks in Southern Ethiopia, 2020 |
| title_full_unstemmed | Isolation and Molecular Characterization of Peste des Petits Ruminants Virus from Outbreaks in Southern Ethiopia, 2020 |
| title_short | Isolation and Molecular Characterization of Peste des Petits Ruminants Virus from Outbreaks in Southern Ethiopia, 2020 |
| title_sort | isolation and molecular characterization of peste des petits ruminants virus from outbreaks in southern ethiopia 2020 |
| url | http://dx.doi.org/10.1155/2022/5329898 |
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