Development of a sensitive antibody-based label-free electrochemical immunosensors for breast cancer diagnosis
Early detection of human epidermal growth factor receptor 2 (HER2) is crucial for effective breast cancer treatment. This research focuses on the development of a sensitive, label-free electrochemical immunosensor for detecting HER2, which plays a key role in breast cancer diagnosis. The sensor empl...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | Sensing and Bio-Sensing Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214180425000595 |
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| author | Saiedeh Agh Atabay Hossein Ahmadzadeh Parviz Norouzi Somayeh Dianat Kheibar Dashtian |
| author_facet | Saiedeh Agh Atabay Hossein Ahmadzadeh Parviz Norouzi Somayeh Dianat Kheibar Dashtian |
| author_sort | Saiedeh Agh Atabay |
| collection | DOAJ |
| description | Early detection of human epidermal growth factor receptor 2 (HER2) is crucial for effective breast cancer treatment. This research focuses on the development of a sensitive, label-free electrochemical immunosensor for detecting HER2, which plays a key role in breast cancer diagnosis. The sensor employs a gold electrode functionalized with carboxylic acid molecules (11-mercaptoundecanoic acid, 3-mercaptopropionic acid), activated by coupling agents and modified with anti-HER2 antibodies. Key parameters such as pH, incubation time, and antibody concentration were optimized to enhance the immunosensor's performance. Using differential pulse voltammetry (DPV), the immunosensor achieved a limit of detection (LOD) of 2.3 fM within a range of 0.10 nM to 100 nM. To further improve detection limits and sensitivity, protein G (PG) was incorporated into the next iteration of the immunosensor. Again, a gold electrode was used as the main component, this time functionalized with L-cysteine (CYS) and PG. The PG-enhanced sensor exhibited improved sensitivity and a LOD of 0.51 fM, with a linear range from 1 fM to 0.10 nM. The sensor demonstrated high selectivity for HER2 and maintained performance in human serum samples. These results highlight its potential for rapid diagnostic applications in breast cancer monitoring. |
| format | Article |
| id | doaj-art-5b41927289fb44f299be60a06ee38ebd |
| institution | DOAJ |
| issn | 2214-1804 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Sensing and Bio-Sensing Research |
| spelling | doaj-art-5b41927289fb44f299be60a06ee38ebd2025-08-20T03:21:01ZengElsevierSensing and Bio-Sensing Research2214-18042025-06-014810079310.1016/j.sbsr.2025.100793Development of a sensitive antibody-based label-free electrochemical immunosensors for breast cancer diagnosisSaiedeh Agh Atabay0Hossein Ahmadzadeh1Parviz Norouzi2Somayeh Dianat3Kheibar Dashtian4Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad 9177948974, IranDepartment of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad 9177948974, Iran; Corresponding authors.Center of Excellence in Electrochemistry, School of Chemistry, University of Tehran, Tehran, IranDepartment of Chemistry, Faculty of Sciences, University of Hormozgan, Bandar Abbas 79161-93145, IranDepartment of Semiconductors, Materials and Energy Research Center (MERC), P.O. Box 31787-316, Karaj, IranEarly detection of human epidermal growth factor receptor 2 (HER2) is crucial for effective breast cancer treatment. This research focuses on the development of a sensitive, label-free electrochemical immunosensor for detecting HER2, which plays a key role in breast cancer diagnosis. The sensor employs a gold electrode functionalized with carboxylic acid molecules (11-mercaptoundecanoic acid, 3-mercaptopropionic acid), activated by coupling agents and modified with anti-HER2 antibodies. Key parameters such as pH, incubation time, and antibody concentration were optimized to enhance the immunosensor's performance. Using differential pulse voltammetry (DPV), the immunosensor achieved a limit of detection (LOD) of 2.3 fM within a range of 0.10 nM to 100 nM. To further improve detection limits and sensitivity, protein G (PG) was incorporated into the next iteration of the immunosensor. Again, a gold electrode was used as the main component, this time functionalized with L-cysteine (CYS) and PG. The PG-enhanced sensor exhibited improved sensitivity and a LOD of 0.51 fM, with a linear range from 1 fM to 0.10 nM. The sensor demonstrated high selectivity for HER2 and maintained performance in human serum samples. These results highlight its potential for rapid diagnostic applications in breast cancer monitoring.http://www.sciencedirect.com/science/article/pii/S2214180425000595Breast cancerDifferential pulse voltammetry (DPV)HER2 biomarkerLabel-free |
| spellingShingle | Saiedeh Agh Atabay Hossein Ahmadzadeh Parviz Norouzi Somayeh Dianat Kheibar Dashtian Development of a sensitive antibody-based label-free electrochemical immunosensors for breast cancer diagnosis Sensing and Bio-Sensing Research Breast cancer Differential pulse voltammetry (DPV) HER2 biomarker Label-free |
| title | Development of a sensitive antibody-based label-free electrochemical immunosensors for breast cancer diagnosis |
| title_full | Development of a sensitive antibody-based label-free electrochemical immunosensors for breast cancer diagnosis |
| title_fullStr | Development of a sensitive antibody-based label-free electrochemical immunosensors for breast cancer diagnosis |
| title_full_unstemmed | Development of a sensitive antibody-based label-free electrochemical immunosensors for breast cancer diagnosis |
| title_short | Development of a sensitive antibody-based label-free electrochemical immunosensors for breast cancer diagnosis |
| title_sort | development of a sensitive antibody based label free electrochemical immunosensors for breast cancer diagnosis |
| topic | Breast cancer Differential pulse voltammetry (DPV) HER2 biomarker Label-free |
| url | http://www.sciencedirect.com/science/article/pii/S2214180425000595 |
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