The effect of Euonymus Fortunei extract combined with oxaliplatin on the IKKβ/NF-κB signaling pathway in colon cancer
[Objectives] To investigate the effects of Euonymus Fortunei extract combined with oxaliplatin on tumor growth and the expression of inflammatory cytokines in colon cancer. [Methods] BALB/c nude mice were subcutaneously injected with CT26 colon cancer cells to establish a xenograft model of colon ca...
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| Format: | Article |
| Language: | zho |
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Editorial Office of Journal of Colorectal & Anal Surgery
2024-10-01
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| Series: | 结直肠肛门外科 |
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| Online Access: | https://jcas.gxmuyfy.cn/cn/wqll/paper.html?id=361&cateName=2024%E5%B9%B4%20%E7%AC%AC30%E5%8D%B7%20%E7%AC%AC5%E6%9C%9F |
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| author | Liu Jiaxuan Ju Yongle Liao Jiabao Ouyang Manzhao Luo Zhentao Liao Tianyou Huang Huacong |
| author_facet | Liu Jiaxuan Ju Yongle Liao Jiabao Ouyang Manzhao Luo Zhentao Liao Tianyou Huang Huacong |
| author_sort | Liu Jiaxuan |
| collection | DOAJ |
| description | [Objectives] To investigate the effects of Euonymus Fortunei extract combined with oxaliplatin on tumor growth and the expression of inflammatory cytokines in colon cancer. [Methods] BALB/c nude mice were subcutaneously injected with CT26 colon cancer cells to establish a xenograft model of colon cancer. Thirty-two nude mice were randomly divided into four groups: the tumor-bearing group, the oxaliplatin intervention group, the Euonymus Fortunei intervention group, and the combined intervention group (Euonymus Fortunei combined with oxaliplatin), with 8 mice in each group. The interventions were continuously administered for 3 weeks. Tumor volume, tumor mass were measured, and the tumor inhibition rate was calculated. The levels of IL-1, IL-6, and TNF-α in serum were measured using ELISA. The expression of IKKβ and NF-κB proteins in tumor tissues was detected by Western blotting. [Results] Compared with the tumor-bearing group, the Euonymus Fortunei intervention group showed no significant difference in tumor volume and tumor mass (P>0.05), but the levels of IL-1, IL-6, and TNF-α in serum and the relative expression of IKKβ and NF-κB proteins in tumor tissues were significantly reduced (P<0.05). Compared with the oxaliplatin intervention group, the combined intervention group showed significantly reduced tumor volume and tumor mass (P<0.05), and a significantly increased tumor inhibition rate (69.26%). The levels of IL-1, IL-6, and TNF-α in serum and the relative expression of IKKβ and NF-κB proteins in tumor tissues were significantly reduced (P<0.05). [Conclusion] Although monotherapy with Euonymus Fortunei did not significantly inhibit tumor growth in colon cancer, its combination with oxaliplatin showed a significant inhibitory effect on tumor growth. This effect may be related to the regulation of the IKKβ/NF-κB signaling pathway by Euonymus Fortunei extract. |
| format | Article |
| id | doaj-art-5b336d4d6370484fbb18c68b6dec47e4 |
| institution | OA Journals |
| issn | 1674-0491 |
| language | zho |
| publishDate | 2024-10-01 |
| publisher | Editorial Office of Journal of Colorectal & Anal Surgery |
| record_format | Article |
| series | 结直肠肛门外科 |
| spelling | doaj-art-5b336d4d6370484fbb18c68b6dec47e42025-08-20T02:31:02ZzhoEditorial Office of Journal of Colorectal & Anal Surgery结直肠肛门外科1674-04912024-10-0130560761110.19668/j.cnki.issn1674-0491.2024.05.016The effect of Euonymus Fortunei extract combined with oxaliplatin on the IKKβ/NF-κB signaling pathway in colon cancerLiu Jiaxuan0Ju Yongle1Liao Jiabao2Ouyang Manzhao3Luo Zhentao4Liao Tianyou5Huang Huacong6Department of Gastrointestinal Surgery, Shunde Hospital of Southern Medical University (The First People’s Hospital of Shunde), Foshan 528300, Guangdong, ChinaDepartment of Gastrointestinal Surgery, Shunde Hospital of Southern Medical University (The First People’s Hospital of Shunde), Foshan 528300, Guangdong, ChinaDepartment of Gastrointestinal Surgery, Shunde Hospital of Southern Medical University (The First People’s Hospital of Shunde), Foshan 528300, Guangdong, ChinaDepartment of Gastrointestinal Surgery, Shunde Hospital of Southern Medical University (The First People’s Hospital of Shunde), Foshan 528300, Guangdong, ChinaDepartment of Gastrointestinal Surgery, Shunde Hospital of Southern Medical University (The First People’s Hospital of Shunde), Foshan 528300, Guangdong, ChinaDepartment of Gastrointestinal Surgery, Shunde Hospital of Southern Medical University (The First People’s Hospital of Shunde), Foshan 528300, Guangdong, ChinaDepartment of Gastrointestinal Surgery, Shunde Hospital of Southern Medical University (The First People’s Hospital of Shunde), Foshan 528300, Guangdong, China[Objectives] To investigate the effects of Euonymus Fortunei extract combined with oxaliplatin on tumor growth and the expression of inflammatory cytokines in colon cancer. [Methods] BALB/c nude mice were subcutaneously injected with CT26 colon cancer cells to establish a xenograft model of colon cancer. Thirty-two nude mice were randomly divided into four groups: the tumor-bearing group, the oxaliplatin intervention group, the Euonymus Fortunei intervention group, and the combined intervention group (Euonymus Fortunei combined with oxaliplatin), with 8 mice in each group. The interventions were continuously administered for 3 weeks. Tumor volume, tumor mass were measured, and the tumor inhibition rate was calculated. The levels of IL-1, IL-6, and TNF-α in serum were measured using ELISA. The expression of IKKβ and NF-κB proteins in tumor tissues was detected by Western blotting. [Results] Compared with the tumor-bearing group, the Euonymus Fortunei intervention group showed no significant difference in tumor volume and tumor mass (P>0.05), but the levels of IL-1, IL-6, and TNF-α in serum and the relative expression of IKKβ and NF-κB proteins in tumor tissues were significantly reduced (P<0.05). Compared with the oxaliplatin intervention group, the combined intervention group showed significantly reduced tumor volume and tumor mass (P<0.05), and a significantly increased tumor inhibition rate (69.26%). The levels of IL-1, IL-6, and TNF-α in serum and the relative expression of IKKβ and NF-κB proteins in tumor tissues were significantly reduced (P<0.05). [Conclusion] Although monotherapy with Euonymus Fortunei did not significantly inhibit tumor growth in colon cancer, its combination with oxaliplatin showed a significant inhibitory effect on tumor growth. This effect may be related to the regulation of the IKKβ/NF-κB signaling pathway by Euonymus Fortunei extract.https://jcas.gxmuyfy.cn/cn/wqll/paper.html?id=361&cateName=2024%E5%B9%B4%20%E7%AC%AC30%E5%8D%B7%20%E7%AC%AC5%E6%9C%9Fcolon cancereuonymus fortuneioxaliplatinikkβ/nf-κb signaling pathway |
| spellingShingle | Liu Jiaxuan Ju Yongle Liao Jiabao Ouyang Manzhao Luo Zhentao Liao Tianyou Huang Huacong The effect of Euonymus Fortunei extract combined with oxaliplatin on the IKKβ/NF-κB signaling pathway in colon cancer 结直肠肛门外科 colon cancer euonymus fortunei oxaliplatin ikkβ/nf-κb signaling pathway |
| title | The effect of Euonymus Fortunei extract combined with oxaliplatin on the IKKβ/NF-κB signaling pathway in colon cancer |
| title_full | The effect of Euonymus Fortunei extract combined with oxaliplatin on the IKKβ/NF-κB signaling pathway in colon cancer |
| title_fullStr | The effect of Euonymus Fortunei extract combined with oxaliplatin on the IKKβ/NF-κB signaling pathway in colon cancer |
| title_full_unstemmed | The effect of Euonymus Fortunei extract combined with oxaliplatin on the IKKβ/NF-κB signaling pathway in colon cancer |
| title_short | The effect of Euonymus Fortunei extract combined with oxaliplatin on the IKKβ/NF-κB signaling pathway in colon cancer |
| title_sort | effect of euonymus fortunei extract combined with oxaliplatin on the ikkβ nf κb signaling pathway in colon cancer |
| topic | colon cancer euonymus fortunei oxaliplatin ikkβ/nf-κb signaling pathway |
| url | https://jcas.gxmuyfy.cn/cn/wqll/paper.html?id=361&cateName=2024%E5%B9%B4%20%E7%AC%AC30%E5%8D%B7%20%E7%AC%AC5%E6%9C%9F |
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